Chapter 5
Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
60
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
3.
Allow the ROI calibration plate to warm to room
temperature (approximately 5 minutes).
IMPORTANT!
Do not remove an ROI calibration
plate from its packaging until you are ready to
run it. The fluorescent dye in the wells of the
plate is photosensitive. Prolonged exposure to
light can diminish the fluorescence signal
strength of the plate.
4.
Remove the ROI calibration plate from its
packaging.
IMPORTANT!
Do not discard the packaging for
the ROI calibration plate. The plate can be used
up to three times if it is stored in its original
packaging sleeve.
5.
Standard plates only
: Vortex the plate for
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
6.
Briefly centrifuge the ROI calibration plate in a
centrifuge with a plate adapter (<1500x
g
).
7.
Verify that the liquid in each well of the
background plate is positioned at the bottom of
the well. If not, centrifuge the plate again at a
higher rpm and for a longer period of time.
>00:05:00
Ambient
00:00:05
<1500
×
g
Correct
Incorrect
Liquid is
positioned at
bottom of well.
• Not centrifuged with enough force,
or
• Not centrifuged for enough time
