Ankom 2000, Руководство оператора

Страница: 38 / 44

Operator’s Manual
pg. 38
Rev 05/12/21
Neutral Detergent Fiber in Feeds - Filter Bag Technique (for A2000 and A2000I)
Definition-
This method determines Neutral Detergent Fiber, which is the residue remaining after digesting in a detergent solution. The fiber
residues are predominantly hemicellulose, cellulose, and lignin.
Scope -
This method is applicable to grains, feeds, forages, and all fiber-bearing material.
Apparatus
1. Analytical Balance—capable of weighing 0.1 mg.
2. Oven—capable of maintaining a temperature of 102 ± 2°C.
3. Digestion instrument—capable of performing the digestion at
100 ± 0.5°C and maintaining a pressure of 10-25psi. The
instrument must be capable of creating a similar flow around
each sample to ensure uniformity of extraction (ANKOM
2000
with 65rpm agitation, ANKOM Technology).
4. Filter Bags—constructed from chemically inert and heat resistant
filter media, capable of being heat sealed closed and able to
retain 25 micron particles while permitting solution penetration
(F57 and F58, ANKOM Technology).
5. Heat sealer—sufficient for sealing the filter bags closed to ensure
complete closure (HS or HSi, ANKOM Technology).
6. Desiccant Pouch—collapsible sealable pouch with desiccant
inside that enables the removal of air from around the filter bags
(
MoistureStop weigh pouch, ANKOM Technology).
7. Marking pen—solvent and acid resistant (F08, ANKOM
Technology).
Reagents
1. Neutral Detergent Solution—Add 30g Sodium dodecyl sulfate
(USP),
18.61g Ethylenediaminetetraacetic disodium salt
(dehydrate), 6.81g Sodium borate, 4.56g Sodium phosphate
dibasic (anhydrous), and 10.0ml Triethylene glycol to 1L
distilled H
2
O (premixed chemical solution available from
ANKOM Technology). Check that pH is from 6.9 to 7.1. Agitate
and heat to aid solution.
CAUTION1:
Powdered chemicals will irritate mucous
membranes. A dust mask and gloves should be worn when
handling these chemicals.
2. Alpha-amylase—Heat-stable bacterial alpha-amylase: activity =
17,400 Liquefon Units / ml (FAA, ANKOM Technology).
3. Sodium sulfite—Na
2
SO
3
, anhydrous (FSS, ANKOM
Technology)
Sample Preparation
Grind samples in a centrifugal mill with a 2mm screen or cutter type
(Wiley) mill with a 1mm screen. Samples ground finer may have
particle loss from the filter bags and result in low values.
NDF Procedure
(see the NDF Analysis section of the Operator’s M anual for
more detail)
1. Use a solvent resistant marker to label the filter bags to be used in
the analysis.
2. Weigh and record the weight of each empty filter bag (W
1
) and
zero the balance. NOTE: Do not pre-dry filter bags. Any moisture
will be accounted for by the blank bag correction.
3. Place 0.45 – 0.50g of prepared sample in up to 23 of the bags and
record the weight (W
2
) of each. Avoid placing the sample in the
upper 4mm of the bag.
4. Include at least one empty bag in the run to determine the
blank bag correction (C
1
).
NOTE: A running average blank bag correction factor (C
1
)
should be used in the calculation of fiber. The inclusion of at
least one blank bag in each run is mainly used as an indicator
of particle loss. A C
1
larger than 1.0000 indicates that sample
particles were lost from filter bags and deposited on the blank
bag during the extraction. Any fiber particle loss from the
filter bags will generate erroneous results. If particle loss is
observed then the grinding method needs to be evaluated.
5. Using a heat sealer, completely seal each filter bag closed
within 4mm of the top to encapsulate the sample. NOTE: Use
sufficient heat to completely seal the filter bags and allow
enough cool time (2 sec) before removing each bag from the
heat sealer.
6. Pre-extract only samples containing >5% fat: Extract
samples by placing bags with samples into a container with a
top. Pour enough acetone into the container to cover the bags
and secure the top.
CAUTION2: Acetone is extremely flammable. Avoid static
electricity and use a fume hood when handling.
Shake the container 10 times and allow bags to soak for 10
minutes. Repeat with fresh acetone. Pour out acetone and
place bags on a wire screen to air-dry.
Exception – Roasted soybean: Due to the processing of
roasted soy a modification to the extraction is required. Place
roasted soy samples into a container with a top. Pour enough
acetone into the container to cover the bags and secure the
top. Shake the container 10 times and pour off the acetone.
Add fresh acetone and allow samples to soak for twelve
hours. After the soak time, pour out the acetone and place the
bags on a wire screen to dry.
7. Spread the sample uniformly inside the filter bags by shaking
and flicking the bags to eliminate clumping.
8. Place up to 3 bags on each of eight Bag Suspender Trays
(maximum of 24 bags). Stack the trays on the center post of
the Bag Suspender with each level rotated 120 degrees in
relation to the tray below it. Place the empty 9th tray on top.
NOTE: All nine trays must be used regardless of the number
of bags being processed.
9. Verify that the hot water supply is on and the drain hose is
securely positioned in the drain.
10. If you are using Cubetainers for your chemicals, attach the
ND solution hose to the Cubetainer and then to Port A on the
instrument.
(Procedure continued on next page.)