Ankom 2000, Руководство оператора

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Operator’s Manual
Rev 05/12/21
pg. 23
20. Remove the bags from the beaker and place them on a wire screen to air-dry.
Hazardous Materials
–
Do NOT place bags in an oven until all acetone has
evaporated.
21. Place air-dried bags in the oven and heat at 102°C ± 2° for 2 – 4 hours (depending on the oven).
When running a lignin procedure or a sequential (NDF/ADF or
NDF/ADF/Lignin) with F57 Filter Bags it is important not to dry the bags
overnight after the NDF or ADF procedure. A drying timeframe of 2-4 hours
at 100°C to 105°C is sufficient to thoroughly dry the bags after each
procedure. Extended drying times or too high a temperature can compromise
the bag’s filtration media. In addition, after the fourth rinse during the ADF
procedure if you would like to confirm the acid has been completely rinsed
from the bags, capture some water from the waste tube and check the water
with litmus paper
.
22. Remove the bags from the oven and place them in a Desiccant Pouch.
Do NOT use conventional countertop or cabinet desiccators for this analysis.
23. Allow the bags to cool to room temperature. This should take about 10 – 15 minutes.
24. Remove one filter bag from the Desiccant Pouch. Press the pouch to remove ambient air and zip it tight.
25. Re-weigh the filter bag (W
3
) immediately.
26. Repeat steps 24 and 25 for each filter bag in the Desiccant Pouch.
27. Calculate NDF using the following formula.
% NDF (as-received basis) = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of filter bag with fiber after extraction process
C
1
= Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
Amylase is sticky. Unless you are running multiple NDF procedures in near
succession, you must flush the system after NDF procedures. See the “Flush
Procedure” section of this manual for details.