Ankom 2000, Руководство оператора

Страница: 37 / 44

Operator’s Manual
Rev 05/12/21
pg. 37
9. Verify that the hot water supply is on and the drain hose is securely
positioned in the drain.
10. Read the Temperature Controller on the right side of the
instrument. If the temperature is higher than 20°C, cool the Vessel
as follows:
a. Fill the Vessel with cold water.
b. When the Temperature Controller reads 20°C, run the
Flush Procedure to drain the water.
c. Repeat steps a and b if necessary.
ADF Procedure (continued)
11. If you are using Cubetainers for your chemicals, attach the AD
solution hose to the Cubetainer and then to Port B on the
instrument.
NOTE: If you do not want to use the Cubitainers to automatically
add your solutions, you can manually fill the vessel for each
procedure. First make sure that the hoses to the Cubitainers are
disconnected. Follow steps 12 and 13a below. Then press START
on the Keypad and immediately pour 2L of solution directly into
the vessel. Please note that the solution must cover the level sensor
in order for the instrument to start its operation. Once agitation
begins, close the Vessel Lid and follow the rest of the procedure
from step 14 to the end.
12. Open the Vessel Lid and insert the Bag Suspender with bags into
the Vessel and place the Bag Suspender Weight on top of the
empty 9
th
tray to keep the Bag Suspender submerged.
13. Follow the instructions on the ANKOM
2000
display:
a. Select ADF.
b. Close the Vessel Lid.
c. Confirm hot water is on (>70°C).
d. Press START.
14. When the ADF extraction and rinsing procedures are complete,
open the Vessel Lid and remove the filter bags. Gently press out
excess water from the bags. Place bags in a 250ml beaker and add
enough acetone to cover bags and soak for 3-5 minutes.
15. Remove the filter bags from the acetone and place them on a wire
screen to air-dry. Completely dry in an oven at 102 ± 2°C. (In most
ovens the filter bags will be completely dry within 2-4 hours.) Do
not place bags in the oven until the acetone has completely
evaporated.
NOTE: When running a lignin procedure or a sequential
(NDF/ADF or NDF/ADF/Lignin) with the F57 Filter Bag it is
important not to dry the bags overnight after the NDF or ADF
procedure. A drying timeframe of 2-4 hours at 100°C to 105°C is
sufficient to thoroughly dry the bags after each procedure.
Extended drying times or too high a temperature can compromise
the bag’s filtration media. In addition, be sure to check the water
of the fourth rinse during the ADF procedure to ensure all the
sulfuric acid has been removed from the bags. If litmus paper
shows the presence of acid during the fourth hot water rinse, repeat
until neutral.
16. Remove the filter bags from the oven and immediately place them
directly into a collapsible desiccant pouch and flatten to remove
any air. Cool to ambient temperature and weigh the filter bags
(W
3
). NOTE: Do not use a conventional desiccator container.
Calculations
% ADF (as-received
basis) = 100 x (W
3
– (W
1
x
C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of bag with fiber
after extraction process
C
1
= Blank bag correction (running
average of final oven-dried
weight divided by original blank
bag weight)