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Operator’s Manual
Rev 05/12/21
pg. 21
NDF Analysis Procedure using the ANKOM
2000
Fiber Analyzer
To perform NDF analysis on prepared samples, follow the procedure detailed below.
•
If you are changing from one analytical method to another, flush the
system before running. See the “Flush Procedure” section of this manual
for more information.
•
Amylase tends to be sticky. You should always clean the Amylase
Dispenser Assembly and flush Port B after every NDF procedure unless
you are running another NDF procedure with less than two hours
between procedures.
1.
Verify that the hot water supply is on and the drain hose is securely positioned in the drain.
2.
If you are using Cubitainers for your chemicals, attach the ND solution
hose first to the Cubitainer and then to Port A on the instrument.
The solutions are gravity fed into the instrument ports. The bottom of the
solution level must be at least 5” (13cm) above the port. The top of the
solution level can be no more than 20” (50cm) above either port or the
solution will overflow into the drain.
3.
Open the Vessel Lid.
4.
Place the Bag Suspender with the samples into the Vessel.
5.
Place the Bag Suspender Weight onto the Bag Suspender rod to hold the trays in place.
6.
Attach the Amylase Dispenser Assembly to Port B. This will be used to
automatically add amylase to the vessel during the rinses.
7.
Add water until the dispenser is 25% full.
8.
Add two capfuls (8ml) of amylase to the dispenser.
9.
Add water until the dispenser is 75% full.
10.
Turn the instrument Power Switch to the ON position. The Display will light and allow you to select an
analysis procedure.
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