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Andor Technology Zyla sCMOS 4.2 PLUS, Руководство по оборудованию

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Andor Technology Zyla sCMOS 4.2 PLUS Скачать руководство пользователя страница 54

version 2.0 rev 18 Feb 2021

54

2.7.4.1  Live Mode

Live Mode

 refers to a version of 

Run Till Abort

 in which each user frame will be the latest frame output by the 

sensor and will have the minimum amount of latency through the camera as possible.

Live mode requires the use of SW Trigger. In order to ensure that frames are buffered in the on-camera memory 

for the shortest possible time, the next SW trigger must not be sent until the user frame has been transmitted 
from the camera. This ensures that the on-camera memory only stores a single user frame at a time and no 
additional latency builds up. 

NOTE: The frame rate achievable in Live Mode is dependent on the performance of the system that the 
camera is attached to.

2.7.5 Fast Exposure Switch 

During an acquisition the user can change the exposure time, within allowable limits. Once a new exposure 
value has been written, it will be applied to the next user frame after the current frame exposure has 

completed. The exposure time can be changed any number of times before the acquisition finishes

2.7.6 Frame Rate Control 

If Internal Trigger is being used, the camera will trigger the sensor at the fastest possible rate by default. The 

user can reduce this trigger rate by defining a Frame Rate that is less than the maximum possible rate. Frame 

Rate must be set before the acquisition starts.

Note: This is currently only applicable to ‘long exposures’.

2.7.7  LightScan PLUS

LightScan PLUS describes a functionality of the Zyla 4.2 PLUS camera that allows the end-user to have more 
control over the Rolling Shutter scanning mechanism that is inherent to CMOS sensors. This scanning mode can 
be readily adapted to niche optical modalities, such as Scanned Light Sheet microscopy or Line Scan Confocal 
Microscopy.

2.7.7.1 Multiple Readout Directions

The standard mode of operation in sCMOS cameras is to read out from the centre of the sensor out to the 
edge with two halves of the sensor exposing simultaneously. LightScan PLUS provides the user with a range 

of different rolling shutter readout options. Firstly, the rolling shutter can now be scanned from the top to the 

bottom of the sensor or vice versa in one continuous sweep (Figure 34), adapting the sensor to single port 
readout. It should be acknowledged however that this functionality will halve the rolling shutter frame rate, 
resulting in a maximum full resolution frame rate of 50 fps.

Figure 34: LightScan PLUS enables the rolling shutter to scan the sensor from top to bottom or vice versa in one continuous sweep. This 

is also known as Single Port Readout.

Содержание Zyla sCMOS 4.2 PLUS

Страница 1: ...2 sensor Lowest Noise Imaging EMCCD Version 2 0 rev 18 Feb 2021 Hardware Guide Covers Zyla 4 2 PLUS and 5 5 models with Camera Link or USB 3 connections and air or water cooling andor com Andor Techno...

Страница 2: ...tter 17 2 2 1 Rolling Shutter 18 2 2 2 Global Shutter Zyla 5 5 only 19 2 2 3 Selecting Rolling or Global Shutter 20 2 2 3 1 Examples of Typical Applications for Global Shutter Mode 20 2 2 4 Rolling an...

Страница 3: ...a 4 2 Only 41 2 6 2 12 Rolling Shutter Triggering Constraints 42 2 6 3 Global Shutter Triggering Modes Zyla 5 5 only 43 2 6 3 1 Global Shutter Internal Triggering Non Overlap Mode 44 2 6 3 2 Global Sh...

Страница 4: ...ng Setting BIOS options for PCs not supplied by Andor 61 3 8 Software Support 61 SECTION 4 OPERATION 62 4 1 Connecting your Camera and Switching it on 62 4 2 Using the Lens Mount Adaptors 63 4 2 1 C m...

Страница 5: ...5 Camera does not cool to the required temperature 69 6 6 Fire Aux_Out and Arm outputs not functioning correctly 70 6 7 External Trigger input not functioning correctly 70 6 8 Condensation Appearing...

Страница 6: ...radio frequency generators X ray instruments and pulsed discharge optical sources may compromise performance if shielding of the Zyla is inadequate 13 This product is not designed to provide protectio...

Страница 7: ...tted to air cooled units from February 2015 onwards max Ambient temperature changed to 30o C Edited Mechanical drawing for water cooled models to show distance from C mount to camera faceplate when in...

Страница 8: ...vailable in both air cooled and water cooled forms and with Camera Link and USB 3 0 connection options Figure 1 The Zyla sCMOS Camera Left Water Cooled model right air cooled model The Zyla sCMOS came...

Страница 9: ...Andor Technology 300 Baker Avenue Suite 150 Concord MA 01742 USA Tel 1 860 290 9211 Fax 1 860 290 9566 Asia Pacific China Andor Technology Japan 5F IS Building 3 32 42 Higashi Shinagawa Tokyo 140 000...

Страница 10: ...THE INFORMATION PROVIDED HEREIN COPYRIGHT AND PROTECTIVE NOTICES The copyright in this document and the associated drawings are the property of Andor Technology and all rights are reserved This docume...

Страница 11: ...t Kit SDK Solis Image Capture and Analysis Software Note Longer lengths of USB 3 0 cable are available from other suppliers such as Stemmer CEI Please see the Zyla specifications or contact Andor or y...

Страница 12: ...certified mains lead with an IEC female plug for connection to the mains electrical supply The Zyla PSU is for use with Telecommunications Computer Industrial Controller and OA Systems and must only...

Страница 13: ...e FIRE pulses associated with Row 1 and Row n the last row read out in the image frame Therefore the FIRE ALL pulse represents the time within a frame when all rows on the sensor are simultaneously ex...

Страница 14: ...A 4 pin power connector is fitted for power connection details are shown below Figure 5 Left Camera Connection and Right Connector socket Connector Cable Plug P N 6 1437719 4 Socket P N 3 1437719 3 TE...

Страница 15: ...information 1 6 1 Important Considerations when using Cooling Systems Always ensure that the temperature of the liquid coolant circulated through the camera head is above the dew point of the camera a...

Страница 16: ...ted to an analog voltage inside each pixel amplifier 3 Pixel voltage is transferred to the column bus via a row select signal 4 The analog voltage is then converted to a digital signal via columns of...

Страница 17: ...ch row in sequence during readout whereas in global shutter mode each pixel in the sensor effectively ends the exposure simultaneously However lowest noise and fastest frame rates are achieved from ro...

Страница 18: ...uld have started and ended its exposure 10 ms 1080 rows x 10 s row after the rows at the centre of the sensor when using 560 MHz readout rate Figure 8 Rolling Shutter Exposure and Readout Rolling shut...

Страница 19: ...configured to operate in a continuous overlap mode analogous to Interline CCD whereby an exposure can proceed while the previous exposure is being readout out from the readout nodes of each pixel In t...

Страница 20: ...d Breakdown Spectroscopy LIBS Global readout involves a step that simultaneously transfers the signal charge of each pixel into the corresponding readout node for that pixel This transfer step is 2 s...

Страница 21: ...p 4 to get the real signal Rolling Shutter Mode charge transfer happens on a per row basis therefore each row follows steps 1 5 until the entire sensor is read out The disadvantage of this is that the...

Страница 22: ...ce of each pixel having its own individual readout structure the overall readout noise in CMOS sensors is described as a distribution as exemplified in Figure 10 below which is a representative noise...

Страница 23: ...ough deep TE cooling of the sensor e g 30o C in the Neo it is possible to dramatically minimize the occurrence of such hot pixels within the sensor meaning that these pixels can still be used for usef...

Страница 24: ...000 3 50 000 1 This is because the high sensitivity gain of 1e ADU that is used to reach 3 e noise means that the 16 bit ADC will top out at 65 536 e well short of the 150 000 e available from the pi...

Страница 25: ...continuously spooling through the Camera Link interface and writing to hard disk Table 4 Typical performance of supported gain settings of the Zyla 5 5 Jan 2012 onwards Amplifier Gain Current Andor SD...

Страница 26: ...16 bit 19 29 19 52 19 52 1920 x 1080 Rolling 12 bit 72 79 72 199 76 192 72 107 76 107 16 bit 59 59 72 199 76 192 72 80 76 80 Global 12 bit 35 79 35 97 35 98 16 bit 35 59 35 97 35 80 512 x 512 Rolling...

Страница 27: ...yla 4 2 216 MHz 0 9 N A 540 MHz 1 1 N A Please refer to Section 2 3 for more information on read noise and the camera performance sheet for read noise values at the various readout speeds 2 5 3 ROI Su...

Страница 28: ...e taken changes randomly External Start is a mode where the camera will wait for one external trigger event to occur after the acquisition sequence has been started Once this external trigger event is...

Страница 29: ...the exposure had completed This results in a cycle time of approximately 79 ms or a frame rate of 12 6 fps for this example With acquisition software events shown in Figure 15 and in particular the Ex...

Страница 30: ...output from the camera known as FIRE Row n FIRE ALL The Fire ALL output from the camera indicates when all rows within a frame are being simultaneously exposed FIRE ANY The FIRE ANY output indicates...

Страница 31: ...Width Zyla 5 5 Parameter Sensor Read out Rate 200 MHz 560 MHz EXT Trig Pulse Width 2 clock cycles 19 37 ns 7 04 ns 2 6 2 2 Timing Parameters and Ext Triggering for Zyla 4 2 The timing tables accompan...

Страница 32: ...ny row is exposing Initially the entire sensor is held in a global clear state to ensure that there is no charge build up on the sensor Global Clear goes LOW and a frame read out is initiated This fra...

Страница 33: ...time is the same as the exposure time entered by the user in this scenario Short Exposures When the required exposure time is less than the time it takes to read out a frame Short Exposures the cycle...

Страница 34: ...d This frame is discarded as it does not contain the correct exposure period Reading out this first frame effectively begins the first exposure When the exposure period has completed a signal frame re...

Страница 35: ...Once the trigger event is detected Global Clear goes LOW and a frame read out is initiated This frame is discarded as it does not contain the correct exposure period Reading out a frame effectively b...

Страница 36: ...the next trigger event is detected The external trigger pulse width defines the exposure time for all rows but is only coincident with the exposure time for Row 1 The exposure for Row 2 will be delay...

Страница 37: ...a frame read out is initiated This frame is discarded as it does not contain the correct exposure period Reading out this first frame effectively begins the first exposure When the next positive edge...

Страница 38: ...era will progress as if the camera was in internal trigger mode see Section 2 6 2 3 and 2 6 2 4 The ARM signal indicates to the user when the camera is ready to detect an External Start Trigger Figure...

Страница 39: ...but different exposure end times When the frame has been read out completely the camera is returned to the Global Clear state until the user defined cycle time is achieved or external trigger is dete...

Страница 40: ...r goes LOW a signal frame read out phase begins When the required exposure period for Row 1 has completed a signal frame rolling shutter readout phase begins When the frame has been read out completel...

Страница 41: ...nd of exposure for Row 2 will be delayed by one row time relative to Row 1 Row 3 will be delayed by one row relative to Row 2 and so forth for the remaining rows hence the exposure time for each row i...

Страница 42: ...lear Internal Non Overlap User settable exposure time Cycle Time is read out time for short exposures User settable Cycle time for long exposures Zyla 4 2 only 1 Frame 1 Row to 30 s 1 Row Global Clear...

Страница 43: ...hen the camera is ready to accept another incoming trigger pulse Global Clear Global Shutter uses Global Clear to begin a new exposure When this pulse is HIGH charge is drained from every pixel thus p...

Страница 44: ...he signal frames as shown in Figure 26 In this scenario the reference frame is read out before the Global Clear is performed The negative edge of the Global Clear begins an exposure After the user def...

Страница 45: ...he end of the exposure The Charge Transfer pulse then goes HIGH to transfer charge from all pixels in the sensor Finally the signal frame is then read as shown below Figure 27 Global Shutter Internal...

Страница 46: ...IGH to transfer charge from all pixels in the sensor The signal frame is then read out During this read out a new exposure begins by pulsing the Global Clear Figure 28 Global Shutter Internal Triggeri...

Страница 47: ...he trigger the Global Clear line is pulsed to clear the charge from the sensor The exposure period then starts and lasts for the user defined exposure time The reference frame is read out during the e...

Страница 48: ...will be missed if it is too fast refer to Table 27 for guidelines on time restrictions Figure 30 Global Shutter External Software Triggering Overlap Mode Table 27 Global Shutter External Software Tri...

Страница 49: ...ns The period of the external trigger pulse defines the overall cycle time If the width of the trigger event is less than the frame read out time the falling edge will be missed and a subsequent falli...

Страница 50: ...ternal trigger is less than the minimum period discussed above any positive edge occurring during the Signal and Reference frames will be ignored The ARM signal indicates when positive going edges wil...

Страница 51: ...ger Non Overlap Mode Figure 33 Global Shutter External Start Triggering Table 30 Global Shutter External Start Triggering Timing Parameters Overlap Off Parameter Minimum Maximum Exposure 1 Row 1 Frame...

Страница 52: ...Time is exposure time 2 Frames 2 InterFrames to 30 s 1 Row 2 Sen sor Clocks x External Start Non Overlap User settable exposure time and Cycle Time but sequence initialized via external trigger pulse...

Страница 53: ...type Valid triggers continue to output user frames from the sensor until the defined number of user frames has been reached Frames are transmitted from the camera depending upon the connectivity i e...

Страница 54: ...lt The user can reduce this trigger rate by defining a Frame Rate that is less than the maximum possible rate Frame Rate must be set before the acquisition starts Note This is currently only applicabl...

Страница 55: ...amera receiving an external trigger and the acquisition start This is important when using peripheral devices e g controlling the illumination source 2 7 7 2 FlexiScan Flexiscan functionality allows t...

Страница 56: ...is important to ensure that this is connected to the buildings protective earth system The equipment should be positioned so that the mains supply plug cord can be easily accessed for disconnection 3...

Страница 57: ...the storage conditions are below the freezing point of the coolant Coolant Hose Connectors Two barbed coolant hose inserts replacement part MSC 06489 are supplied as standard suitable for connection...

Страница 58: ...from the hose 2 Hold the latch in and pull the hose backwards 3 The hose should release from the camera connection with little resistance NOTE If the hose does not release ensure that the latch on the...

Страница 59: ...interface you will require the Andor Drivers called SDK3 Switch on the PC insert CD and run the setup exe file on the CD or download location Confirm the version of software Follow the on screen prom...

Страница 60: ...you intend to use 5 Remove the controller card carefully from its protective ESD packaging and firmly press the card connector into the chosen expansion slot and ensure it is securely locked in 6 Maki...

Страница 61: ...step Memory Node Interleaving Set from NUMA to SMP Note This option is only available on larger workstations with 2 physical processors and may have a different name ensure that NUMA is disabled 3 8 S...

Страница 62: ...d the corresponding port on the PCIe card See Figure 36 please refer also to diagram in Section 3 5 for correct PCIe slot connection Notes Andor Camera Link cables are labelled to assist correct insta...

Страница 63: ...n as required Figure 39 Lens mount adaptor fitted to Zyla In this example a CS mount adaptor is shown 4 2 1 C mount Adaptor The C mount adaptor can be adjusted as follows 1 Loosen the 2 locking screws...

Страница 64: ...d It must not be screwed the entire way into the faceplate 3 Lock the CS mount into position using the 2 locking screws and 1 5 mm Allen key NOTE Maximum torque applied must not exceed 0 6 Nm 4 2 3 F...

Страница 65: ...The Zyla sCMOS sensor is cooled using a thermoelectric TE cooler TE coolers are small electrically powered devices with no moving parts making them reliable and convenient A TE cooler is actually a he...

Страница 66: ...ion In case of emergency the disconnecting point of the equipment is the mains power cord connected to the external power supply or the mains socket switch WARNING SWITCH OFF THE POWER AT THE MAINS SO...

Страница 67: ...al are Andor s high end deep cooled Sona Marana Neo and Balor sCMOS platforms For back filled designs one expects a very low level of moisture ingress into the sensor chamber over time pervading throu...

Страница 68: ...environment throughout the working day the laboratory air conditioning may be turned off overnight during which the camera chamber can become exposed to high heat and humidity Under such circumstance...

Страница 69: ...see Sections 3 4 and 3 5 6 3 Buzzer sounds continuously This indicates that an over temperature condition has occurred within the camera Follow the instructions below to rectify this situation 1 Powe...

Страница 70: ...de of the camera body if the cooling water is at too low a temperature or if the water flow is too high The first signs of condensation will usually be visible around the connectors where the water tu...

Страница 71: ...ed for the camera s sensor chamber to be serviced whereupon the chamber will be re backfilled and the desiccant replaced Saturation of desiccant will result in condensation forming on the sensor or wi...

Страница 72: ...33 000 1 33 000 1 Photon Response Non Uniformity PRNU Half light range Low light range 0 01 0 1 Pre defined Region of Interest ROI 2048 x 2048 1920 x 1080 1392 x 1040 512 x 512 128 x 128 1920 x 1080 1...

Страница 73: ...hat it can only be used in non overlap readout mode i e sequential exposure and readout phases rather than simultaneous 8 Zyla USB 3 0 models should work with any modern USB 3 0 enabled PC laptop prov...

Страница 74: ...from a 0 1 source resistance is 1 7 A min peak and a pulse width of 10 ms min measured at half the peak Safety Certified to an appropriate IEC standard e g IEC 62368 1 or IEC 60950 1 and also meets th...

Страница 75: ...APPENDIX B MECHANICAL DRAWINGS Zyla Mechanical Drawings Air Cooled Models Zyla 5 5 3 tap version Zyla 5 5 and Zyla 4 2 10 tap Zyla 5 5 and 4 2 USB 3 Below Zyla Mechanical Drawings for water cooled ver...

Страница 76: ...version 2 0 rev 18 Feb 2021 76...

Страница 77: ...ion is forming will be on the coolant connections entering and exiting the camera Use of coolant at or below the dewpoint can result in permanent damage to the camera head due to formation of condensa...

Страница 78: ...e including warranty conditions will have been made available during the ordering process The current version for the US is available here for all other regions except Japan please click here D 2 Wast...

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