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Standard 25
ICS
3-6
B 40-029 e 01/98
3.3
Setting of transmitted-light phase contrast
The phase contrast technique is mainly used to increase the image contrast of unstained specimens.
As described in chapter 2, the microscope is ready for operation and switched on according to section
3.1.
The microscope must be set for transmitted-light brightfield, as described in section 3.2.
The further steps depend on whether phase contrast equipment A or B is used.
Optimum phase contrast requires a high level of cleanliness! Therefore, clean the front lens of
the objective used, the visible condenser surfaces, the upper cover slip surface and the lower
carrier plate surface of the specimen with particular care and carefully remove grease.
Equipment A
condenser 0.9 Z (3-6/
9
) and Ph 2 phase stop
carrier (3-6/
7
)
phase contrast objectives labelled "Ph 2"
(3-6/
11
), suitable also for transmitted-light
brightfield
Settings:
Place unstained specimen on the mechanical
stage:
Screw phase-contrast objective, e.g. 40x/0.65 Ph
2 (3-6/
11
), into nosepiece and swing it into the
beam path.
Remove condenser front lens 0.9 from the beam
path via lever (3-6/
10
).
Open the luminous-field diaphragm (3-6/
5
) and
the condenser aperture diaphragm (3-6/
8
).
Swing in phase stop carrier (3-6/
7
) with Ph 2
phase stop.
Set the required brightness.
Check whether phase stop centering complies
with figure (3-7/B). For this, remove one
eyepiece and replace it with the diopter or a
centering telescope (3-6/
3
).
If required, center the phase stop via the two
centering screws (3-6/
6
) using the SW 1.5
screwdriver. The diopter or the centering
telescope (3-6/
3
) must then be replaced with the
eyepiece again.
Fig. 3-6
Setting of phase contrast using
condenser 0.9 Z
Summary of Contents for Standard 25 ICS
Page 1: ...Standard 25 ICS Transmitted Light Microscope Operating Manual...
Page 22: ...Standard 25 ICS 2 2 B 40 029 e 01 98...
Page 30: ...Standard 25 ICS 3 2 B 40 029 e 01 98...
Page 46: ...Standard 25 ICS 4 2 B 40 029 e 01 98...
Page 56: ...Standard 25 ICS 4 12 B 40 029 e 01 98...
Page 58: ...Standard 25 ICS A 2 B 40 029 e 01 98...
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