07/05
13
Image optimization
Choosing a lookup table
•
Select
Palette
in the
Image
window of the
scanned image (Fig. 19).
The
Color Palette
window appears.
•
In the
Color Palette List
panel, click on the
Range Indicator
item (Fig. 20).
The scanned image appears in a false-color
presentation (Fig. 19).
If the image is too bright, it appears red on the
screen. Red = saturation (maximum).
If the image is not bright enough, it appears blue
on the screen. Blue = zero (minimum).
Adjusting the laser intensity
•
Set the
Pinhole
to
1
Airy Unit (Fig. 21).
•
Set the
Detector Gain
high.
•
When the image is saturated, reduce AOTF
transmission in the
Excitation
panel (Fig. 21)
using the slider to reduce the intensity of the
laser light at the specimen.
Adjusting gain and offset
•
Increase the
Amplifier Offset
until all blue
pixels disappear, and then make it slightly
positive (Fig. 21).
•
Reduce the
Detector Gain
until the red pixels
only just disappear.
Fig. 19
Image window
Fig. 20
Color Palette window
Fig. 21
Scan Control window, Channel settings
Summary of Contents for LSM 510 Inverted
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