Zeiss LSM 510 Inverted, Brief Operating Manual

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07/05
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Selecting an objective
•
Open the graphical pop-up menu by clicking on
the
Objective button (Fig. 10).
•
Click on the objective you want to select. The
selected objective will automatically move into
the beam path.
Focussing the microscope for transmitted light
•
Open the graphical pop-up menu by clicking on
the
Transmitted Light button (Fig. 11).
•
Click on the On button. Set the intensity of the
Halogen illuminator using the slider.
•
Click on Close to close the pop-up menu.
•
Place specimen on microscope stage. The cover
slip must be facing up.
•
Use the focusing drive of the microscope to
focus the required object plane.
•
Select specimen detail by moving the stage in X
and Y using the XY stage fine motion control.
Setting the microscope for reflected light
•
Click on the Reflected Light button to open
the shutter of the HBO 100 mercury lamp.
•
Click on the Reflector button and select the
desired filter set by clicking on it.
Storing the microscope settings
Microscope settings can be stored and up to 8
buttons assigned for fast retrieval and adjustment
using the
Microscope Settings panel.
The Store button permits existing microscope
configurations to be stored under any name.
The Apply button permits existing stored
microscope configurations to be loaded.
The Delete button permits existing microscope
configurations to be deleted.
The Assign button permits the assignment of a
microscope configuration to a button.
Note: Depending on the microscope
configuration, settings must be done manually if
necessary.
Fig. 10 Microscope Control window,
e.g.: Axiovert 200 M
Fig. 11 Microscope Control window with
Transmitted Light pop-up menu