![background image](http://html.mh-extra.com/html/zeiss/axiocam-105-color/axiocam-105-color_installation-and-reference-manual_927496041.webp)
Image Acquisition
Axiocam 105 color Instal Reference Guide
33
To do this, set a suitable exposure time and the white balance of the
camera.
Set Köhler illumination on the microscope (if using transmitted light) in
order to create a reproducible image-acquisition situation.
Create a test image of a specimen with quality that is as high as
possible.
Check the quality of the Köhler setting for each objective with which
you want to generate automatic shading correction.
Now move the sample out of the camera’s field of view and bring the
microscope clearly out of focus.
Generate a shading reference image for each objective, one after the
other, by clicking on the
Shading Correction
button on the camera
property page
General
.
If you are using a fully motorized microscope, it is very easy to save
and reload the configuration of the light path setting in a
hardware
setting:
Light intensity
Gray density filter
Setting of the condenser aperture diaphragm and field diaphragm
You can now refocus the microscope and work with the automatic
shading correction.
Notes:
The reference images generated are saved on a user-specific basis. All
users who have their own login must therefore generate their own set
of reference images.
Automatic shading correction only takes into account the changing of
the objective. Changes to the settings of other optical components,
such as an Optovar, the camera adapter or the condenser, are not
taken into account and will make it necessary to repeat the generation
of the reference images.
Summary of Contents for Axiocam 105 color
Page 1: ...Axiocam 105 color Installation Reference 10 2013 October 2013 ...
Page 2: ......
Page 6: ......