Image Acquisition
32
Printed 10.2013
Shading correction to improve image quality
In microscopy, numerous side effects can have an undesirable influence on
image quality. Dust and particles in the beam path, for example, can leave
dark spots in the image, and, under certain conditions, diaphragms and
optics can cause slight shadowing that increases towards the image edge,
due to unavoidable physical effects.
For all Axiocam models it is possible to acquire a shading reference image
and apply this automatically each time an image is acquired. On the
camera property page
General
you will find the
Shading Correction
button. This can be used to trigger the acquisition and saving of a shading
reference image.
If you are using a motorized microscope or a microscope with an encoded
nosepiece, it is possible to acquire a separate reference image for each
objective. These images are then applied automatically for the objective
that has been selected.
Procedure for generating objective-specific shading correction
Prerequisites:
Encoded or motorized microscope stand.
Camera belonging to the Axiocam family.
The following steps are required to set and apply shading correction in the
optimum manner:
Activate the
Automatic
check box on the camera property page
General
.
Make sure that all motorized and encoded microscope components are
correctly configured in the Micro Toolbox. For the automatic application
of shading correction, the correct configuration of the available
objectives and the cameras is particularly important (please also refer
to the MTB2004 Configuration manual).
Set the light intensity with which you want to work.
Summary of Contents for Axiocam 105 color
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