Acquisition Mode
1. Objective – change objectives, this is an
alternative to going to the locate tab.
2. Scan Mode, Frame Size and Line Step –
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Scan Mode - Frame, Line, Spot
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Frame Size – defaults to 512 x 512. Select
Optimal for appropriate number of pixels
depending on numerical aperture and
excitation wavelength.
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Line Step – scan every line or less than
every line.
3. Speed – adjust scan speed. A higher speed with
averaging yields results with the best signal-to-
noise ratio. Use slower scan speeds for better
images, but be aware of photobleaching.
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