Acquisition Mode (cont.)
4. Averaging – improves image by increasing the
signal-to-noise ratio. Select from Line or Frame
averaging and select the number to average by.
Averaging helps to reduce photobleaching.
5. Bit Depth – select from 8 bit (256 gray levels), 12
bit (4096 gray levels), or 16 bit (65536 gray
levels). Be sure your software can handle the
information. 12 and 16 bit images are
recommended for publication and quantitative
measurements.
Direction – x-y scan direction or bi-directional
6. Scan Area – set optical zoom setting and /or
rotate laser orientation.
4
5
6
