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13. Appendix D Sample Preservation 

It is possible to collect samples for later analysis if the situation dictates it. For 
this application the YSI 2315 Preservative Tube Kit 

YSI 2315 Preservative Tube Kit 

YSI 2315 Preservative Tube Kit 

YSI 2315 Preservative Tube Kit is available. The kit 

consists of re-sealable tubes containing a preservative powder and packets of 
YSI 1515 Cell Lysing Agent. 

 WARNING: You must use the YSI Cell Lysing Agent in your 

SPORT when running samples preserved in YSI 
preservative tubes. 

 

You cannot use the YSI preservative tubes unless the lysing agent is used in the 
instrument when the actual measurement takes place. This is due to the fact that 
the preservative tubes contain a detergent. This detergent, when introduced into 
a system without the cell lysing agent, can affect the membrane response and 
cause incorrect readings. While this detergent is also present in the cell lysing 
agent, the response effect is compensated for during normal calibration, and the 
sudden introduction of a sample containing the detergent will have no adverse 
effect. Refer to Appendix E–Effects Of Selected Substances.  

A sample size of 100 microliters minimum is required for each tube. 

Samples preserved in YSI preservative tubes can be stored for up to 48 hours @ 
25°C, and for up to 7 days @ 5°C.  

Summary of Contents for 1500 Sport

Page 1: ...www YSI com User s Manual YSI 1500 Sport L L L L Lactate Analyzer Lactate Analyzer Lactate Analyzer Lactate Analyzer ...

Page 2: ......

Page 3: ...Setting the LCD Contrast 3 12 Displaying Numerical Values in RADIX 3 13 Setting the Stirring Speed 3 13 Selecting the Displayed Units 3 14 Selecting the Operational Mode 3 15 Resetting System Default Parameters 3 16 3 7 Preparing the Fluid System 3 18 3 8 Printer Option 3 19 4 Sample Collection Preparation and Measurement 4 1 4 1 Sample Preparation 4 1 YSI Model 1501 25 microliter Syringepet 4 2 Y...

Page 4: ... 6 14 6 4 Printing Results 6 15 Printing All Sample Results 6 15 Printing Today s Results 6 16 Printing Previous Days Results 6 17 Printing Results By ID Number 6 17 6 5 Transmitting Results Over the RS232 Communication Link 6 18 6 6 Deleting Stored Results 6 19 Editing Results 6 19 Deleting All Results 6 20 6 7 Daily Operational Checks 6 21 7 Instrument Diagnostics 7 1 7 1 Checking Probe Current ...

Page 5: ...echarging the Battery 8 6 8 9 Storing the SPORT 8 6 9 Troubleshooting 9 1 9 1 Fault Messages 9 2 Data System Faults 9 2 Battery Faults 9 4 Enzyme Sensor Faults 9 4 Temperature Faults 9 8 Fluid System Faults 9 9 9 2 Possible Failure Conditions 9 10 10 Appendix A Typical Performance Data 10 1 Capillary Tube Injector YSI Model 1502 10 1 Blunt Needle Syringepet YSI Model 1501 10 3 11 Appendix B Sample...

Page 6: ...3 15 Appendix F Temperature Effect on Operation 15 1 Temperature Effect On Battery Life 15 1 16 Appendix G Accessory Products 16 1 17 Appendix H Cleaning Disinfecting and Decontamination 17 1 18 Appendix I Warranty and Shipping Information 18 1 Cleaning Instructions 18 2 Packing Instructions 18 3 19 Appendix J Required Notice 19 1 ...

Page 7: ...sult accompanying documents Caution indicates that misuse of the instrument could result in mild or serious injury to a person and or damage to equipment Biological Risks Manufacturer Authorized Representative in the European Union In Vitro Diagnostic Medical Device REF 2747 Catalog number 03A6549 Lot number YEAR MO Date of manufacture YEAR MO Use by Date Temperature Limitation ...

Page 8: ...1 1 1 1 2 2 2 2 ...

Page 9: ...ecall and display up to 150 sample values or you can transmit all of the stored data to a personal computer via the RS232 communication port An optional battery powered printer is also available to make hard copy records of the results 2 1 Features Size 13 4 W x 14 8 L x 7 0 H inches 10 lb 33 0 x 27 9 x 17 8 cm 4 53 kg Water resistant splash proof Rechargeable 12 VDC battery w AC adapter Minimum o...

Page 10: ...0 2 mg dL Calibration point 5 mmol L 45 mg dL Typical performance See Appendix A for typical performance data for whole blood and plasma Operating Temperature Range 5 to 45 C 10 to 90 RH non condensing Storage Temperature Range 0 to 60 C 10 to 90 RH non condensing Rechargeable at 95 to 130 VAC with the 115 volt charger or at 190 to 240 VAC with the 230 volt charger 47 to 63 Hz Battery Charger Outp...

Page 11: ... Service or your Dealer Representative immediately Do not discard the shipping container If servicing is required the instrument must be returned to the YSI Repair Center Field service is not available on this instrument We strongly recommend that you use the original shipping container for this purpose as it was designed to protect the instrument during transport Reagents and injection devices fo...

Page 12: ... 2 2 2 3 2 Component Identification This section names the principal parts and describes their use Figure 3 1 and Figure 3 2 identify each part Figure Figure Figure Figure 3 3 3 3 1 1 1 1 SPORT SPORT SPORT SPORT ...

Page 13: ... on an O ring that serves as a fluid seal between the chamber and the probe The temperature probe is in the black holder A small black O ring on the tip of the temperature probe serves as a fluid seal The Injection Port Injection Port Injection Port Injection Port is on the top of the Sample Chamber Inside the port is a sensor that detects the insertion of a YSI injection device A removable cap is...

Page 14: ...t of the Automatic mode You can prepare the reagent solutions for the SPORT yourself These reagents along with a mixing bottle and a graduated syringe 3cc are provided in the YSI Model 1504 Starter Kit which must be ordered separately Please note that the reagents in the starter kit may also be ordered separately IMPORTANT The mixing bottle and Syringe must be cleaned every week See Section 8 2 Pr...

Page 15: ...ds on tightly before putting them back into the instrument If you don t fluid may leak out when you pick up the SPORT by its handle Manual Mode Manual Mode Manual Mode Manual Mode Buffer Solution Buffer Solution Buffer Solution Buffer Solution Fill the 500mL mixing bottle with reagent water Reagent water must be very pure Use high quality deionized or distilled water Add one package of YSI 2357 Bu...

Page 16: ...lastic membrane pack as an applicator press the O ring membrane assembly gently onto the probe face Figure 3 4 Be sure the membrane is seated on the probe face The top of the O ring will be flush with the shoulder Wipe excess salt solution from the probe body then return the probe to the sample chamber Finger tighten the probe retainer so that the O ring makes the necessary seal Return the membran...

Page 17: ... ON PRESS ON If the display prompts you to connect the charger you may do so at this time without turning off the power switch The software will prohibit running until the battery has been sufficiently recharged Refer to Section 9 Troubleshooting for more information If neither of these displays become active press the Reset pushbutton located on the side of the unit If the SPORT fails to respond ...

Page 18: ...ash cycle and brings up the Main menu DELETE DELETE DELETE DELETE DELETE is active only when the display is showing recalled sample values It is used to delete the value showing at the moment NUMERIC KEYPAD NUMERIC KEYPAD NUMERIC KEYPAD NUMERIC KEYPAD The keys numbered from 0 to 9 allow you to enter time date ID numbers menu selections and other data The following sections in this chapter show you...

Page 19: ...ber The third and fourth digits you press define the date you can enter any pair of digits from 01 to 31 The fifth and sixth digits define the last two digits of the desired year When you see the date you want on the screen press ENTER ENTER ENTER ENTER Example January 15 2003 is entered as 01 15 03 01 15 03 01 15 03 01 15 03 ENTER ENTER ENTER ENTER DATE MM DD YY DATE MM DD YY DATE MM DD YY DATE M...

Page 20: ... hour 06 is 6 am and 18 is 6 pm The third and fourth digits you press define the minute you can use anything between 00 and 59 The fifth and sixth digits define the seconds If the time you see on the display is what you want press ENTER ENTER ENTER ENTER Example Nine thirty in the morning would be entered as 09 30 00 ENTER 09 30 00 ENTER 09 30 00 ENTER 09 30 00 ENTER TIME TIME TIME TIME HH MM SS H...

Page 21: ...2 RS232 RS232 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select RS232 by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER DATA DATA DATA DATA 0 BAUD 0 BAUD 0 BAUD 0 BAUD 1 1 1 1 STOP STOP STOP STOP 2 PARITY 2 PARITY 2 PARITY 2 PARITY 3 3 3 3 Select bit data by pressing ENTER ENTER ENTER ENTER 7 BIT 7 BIT 7 BIT 7 BIT 0 0 0 0 8 BIT 8 BIT 8 BIT 8 BIT 1 1 1 1 Select the desired setting by number then ENTER ENTE...

Page 22: ...he previous menu Setting the LCD Contrast Setting the LCD Contrast Setting the LCD Contrast Setting the LCD Contrast Go to Main menu by pressing MENU MENU MENU MENU until you see RUN RUN RUN RUN 0 RECALL 0 RECALL 0 RECALL 0 RECALL 1 1 1 1 SETUP SETUP SETUP SETUP 2 DIAG 2 DIAG 2 DIAG 2 DIAG 3 3 3 3 Select Setup by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER DATE DATE DATE DATE 0 T 0 T 0 T 0 TIME ...

Page 23: ...E 0 TIME 0 TIME 1 1 1 1 RS232 RS232 RS232 RS232 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select MORE by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER LCD LCD LCD LCD 0 RADIX 0 RADIX 0 RADIX 0 RADIX 1 1 1 1 STIR STIR STIR STIR 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select the RADIX mode by pressing 1 1 1 1 then ENTER ENTER ENTER ENTER RADIX RADIX RADIX RADIX PERIOD PERIOD PERIOD PERIOD 0 COMMA 0 COMMA 0 CO...

Page 24: ... ENTER key will speed up the stirring by a preset increment Pressing 1 1 1 1 then ENTER ENTER ENTER ENTER will slow down the stirring by a preset increment Press MENU MENU MENU MENU until the Main menu reappears Selecting the Displayed Units Selecting the Displayed Units Selecting the Displayed Units Selecting the Displayed Units Go to Main menu by pressing M M M MENU ENU ENU ENU until you see RUN...

Page 25: ...Selecting the Operational Mode Go to Main menu by pressing MENU MENU MENU MENU until you see RUN RUN RUN RUN 0 RECALL 0 RECALL 0 RECALL 0 RECALL 1 1 1 1 SETUP SETUP SETUP SETUP 2 DIAG 2 DIAG 2 DIAG 2 DIAG 3 3 3 3 Select Setup by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER DATE DATE DATE DATE 0 TIME 0 TIME 0 TIME 0 TIME 1 1 1 1 RS232 RS232 RS232 RS232 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select Mo...

Page 26: ...em Default Parameters Resetting System Default Parameters If the system is reset all stored results will be erased time date and stir speed will have to be manually reset to correct settings and the analyzer will require recalibration The system will be reset to a series of default settings which are listed below RS232 Communications Baud rate 1200 Data 8 bits Stop bits 1 Parity None Mode Mode Mod...

Page 27: ...DE 0 MODE 0 MODE 1 1 1 1 SET DEFAULT SET DEFAULT SET DEFAULT SET DEFAULT 2 2 2 2 Select Default by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER ARE YOU SURE ARE YOU SURE ARE YOU SURE ARE YOU SURE NO NO NO NO 0 YES 0 YES 0 YES 0 YES 1 1 1 1 To set all system parameters to the default settings press 1 1 1 1 then ENTER ENTER ENTER ENTER The display will read A FEW SECONDS A FEW SECONDS A FEW SECONDS...

Page 28: ...TER You will see this display PRIMING PRIMING PRIMING PRIMING REF ON STIR ON REF ON STIR ON REF ON STIR ON REF ON STIR ON The instrument will run the reference fluid pump for approximately 30 seconds in order to fill the tubing lines Once the cycle has ended you will see this display NOTE NOTE NOTE NOTE The reference pump will only cycle if the selected mode is Automatic if Manual has been selecte...

Page 29: ...es not have the ability to acknowledge the reception of the data Please refer to Figure 3 6 Printer Placement for positioning details Figure Figure Figure Figure 3 3 3 3 6 6 6 6 Printer Placement Printer Placement Printer Placement Printer Placement The instrument has the capability to print the data while running the samples or you may print the results later selectively by date or sample identif...

Page 30: ...ults in memory may be lost or irretrievable Two the additional information on the printout is extremely helpful in detecting performance trends should the need arise for troubleshooting SAMPLE TEMP 22 44 C BASCUR 1 28 nA SAMCUR 21 73 nA REFCUR1 11 12 nA 04 08 98 09 08 001 10 07 mmol L F F F Figure igure igure igure 3 3 3 3 7 7 7 7 Print Format Print Format Print Format Print Format The exact forma...

Page 31: ...g blood samples we refer you to two publications Approved Standard Procedures for the Collection of Diagnostic Blood Specimens by Skin Puncture edited by Jean M Slockbower National Committee for Clinical Laboratory Standards Volume 2 Number 5 pages 132 149 1982 Collection and Handling of Laboratory Specimens A Practical Guide edited by Jean Slockbower and Thomas A Blumenfield J B Lippincott Compan...

Page 32: ...epet 1501 Syringepet 1501 Syringepet Using the Syringepet may feel awkward at first but you must master the technique to get accurate results Practice injecting Calibration standards into the instrument This will allow you to develop the necessary technique while also letting you visually assess how well you are doing by checking the result on the display Depress the plunger all the way with your ...

Page 33: ...Precision and accuracy depend on sample loading technique Any air in the sample column will cause incorrect results Tube Clamp Window Double white band YSI 1505 Capillary Tube Black band Tube clamp Handle Plunger Figure Figure Figure Figure 4 4 4 4 2 2 2 2 1502 Capillary Tube Injector 1502 Capillary Tube Injector 1502 Capillary Tube Injector 1502 Capillary Tube Injector The end of the tube with th...

Page 34: ...le wipe like a Kimwipe Hold the injector directly above the injection port and slide it into the port until you feel it stop In one motion press the plunger all the way down with your thumb Maintaining pressure on the plunger after it has bottomed out remove the injector from the port Release the tube clamp and slide the used tube out of the injector WARNING WARNING WARNING WARNING The used capill...

Page 35: ...zyme Membrane The probe is fitted with a three layer membrane containing immobilized lactate oxidase in the middle layer Figure 5 1 shows an exploded view of the membrane and its relationship to the face of the probe The face of the probe covered by the membrane is situated in a buffer filled sample chamber into which a sample is injected Some of the substrate diffuses through the membrane When it...

Page 36: ...ses H2 O2 but excludes chemical compounds with molecular weights above approximately 200 The cellulose acetate film also protects the platinum surface from proteins detergents and other substances that could foul it However the cellulose acetate film can be penetrated by such compounds as hydrogen sulfide low molecular weight mercaptans hydroxylamines hydrazines phenols and anilines Certain deterg...

Page 37: ...5 5 5 5 3 3 3 3 5 2 Fluid System Figure Figure Figure Figure 5 5 5 5 2 2 2 2 SPORT Fluid System SPORT Fluid System SPORT Fluid System SPORT Fluid System ...

Page 38: ...roximately 30 seconds The reference pump then flushes the chamber for approximately 33 seconds and the sensor response changes to a different value At this point the instrument calculates the relationship of the known standard to the reference solution and stores this information until another calibration injection is performed The Buffer Pump then flushes the chamber for approximately 33 seconds ...

Page 39: ... 5 5 5 5 5 5 Figure Figure Figure Figure 5 5 5 5 3 3 3 3 Automatic Mode Probe Current Profile Automatic Mode Probe Current Profile Automatic Mode Probe Current Profile Automatic Mode Probe Current Profile ...

Page 40: ...obe Current Profile 5 4 Baseline Stability The SPORT checks the probe baseline current for stability whenever it enters the Sample mode The baseline current must be within an acceptable range less than 6 nanoamps in order for the instrument to perform to specification If the baseline current is greater than 6 nA an error message will be displayed see Section 9 Troubleshooting ...

Page 41: ...nual Mode Every day at the beginning of testing Whenever you change the membrane or install new reagent fluids Whenever you turn the instrument power on For maximum accuracy after every fifth sample Whenever you change injection devices Figure Figure Figure Figure 5 5 5 5 5 5 5 5 Calibration Algorithms Calibration Algorithms Calibration Algorithms Calibration Algorithms Calibrating the SPORT in Ma...

Page 42: ...ure 5 5 5 5 6 6 6 6 Aging Membrane Response Aging Membrane Response Aging Membrane Response Aging Membrane Response You can check linearity with YSI 1530 30 mmol L standard after every calibration You can also check it at mid range with YSI 2328 15 mmol L standard Both standards should be injected as if it were a sample Both standards however can be measured as samples any time you want to check t...

Page 43: ...reporting erroneous results Microprocessor Self Test Microprocessor Self Test Microprocessor Self Test Microprocessor Self Test If at initial power up the display does not become active press the reset button If this does not correct the problem try turning the main power switch off and on again If this does not help call YSI Technical Support for repair assistance Also at power up and prior to ea...

Page 44: ...es during sampling to alert you to conditions that may require reconnecting the battery charger The software will not allow continuation below certain levels as the stored data can be corrupted if the battery gets to very low levels Warnings will be displayed at 12 1 volts and a software lockout will occur at 12 0 volts Once the software lockout is enabled the battery charger must be connected Thi...

Page 45: ...rity RS232 LCD Contrast Radix Mode Stir Speed mg dL mmol L Units Measure Auto Manual Operational Mode Format Memory erase Set RS232 Default Start Micro Clock Stir Speed Maximum mg dL System Default Setup Mode Run Ref Cycle Run Wash Cycle Probe Current Chamber Temp Injection Switch Stir Motor Reference Pump Buffer Pump Pumps Infrared Link RS232 Link Print Keypad Display Version Battery Voltage Diag...

Page 46: ...5 5 5 5 1 1 1 12 2 2 2 ...

Page 47: ... be checked several times for accuracy use YSI standards and calibrated as often as required use YSI standards and calibrated as often as required use YSI standards and calibrated as often as required use YSI standards and calibrated as often as required 6 1 Calibration Cycle There are two modes of operation allowed on the SPORT Automatic and Manual mode Each have their own calibration process whi...

Page 48: ...hin the expected range the display will read ERROR BASELINE ERROR BASELINE ERROR BASELINE ERROR BASELINE PRESS MENU PRESS MENU PRESS MENU PRESS MENU If you see this display press MENU MENU MENU MENU then see Section 9 Troubleshooting If there is no problem the display will read INJECT 5mmol L INJECT 5mmol L INJECT 5mmol L INJECT 5mmol L STANDARD STANDARD STANDARD STANDARD Inject the 5 mmol L stand...

Page 49: ...R ON STIR ON STIR ON RUNNING RUNNING RUNNING RUNNING REF ON REF ON REF ON REF ON WASHING WASHING WASHING WASHING CAL CHECK CAL CHECK CAL CHECK CAL CHECK XX XXmmol XX XXmmol XX XXmmol XX XXmmol The acceptable range is from 4 90 to 5 10 mmol L 44 1 to 45 9 mg dL If the result is outside of this range repeat the calibration process in order to eliminate the possibility of improper injection technique...

Page 50: ...SHING WASHING MM DD YY HH MM MM DD YY HH MM MM DD YY HH MM MM DD YY HH MM XXX XX XXmmol L XXX XX XXmmol L XXX XX XXmmol L XXX XX XXmmol L The sample result will be displayed along with the ID in this case 000 date and time Linearity failure means that the instrument is not performing to specifications at the upper limit of the dynamic range This could be due to poor membrane performance check the ...

Page 51: ...ge since the last calibration automatically prompted Prepare buffer and solution and have the 5 mmol L 15 mmol L and 30 mmol L calibration standards provided in the 1504 Starter Kit at hand With the SPORT set up as described in Section 3 3 proceed as follows Load the Syringepet or injector with 5 mmol L standard WARNING WARNING WARNING WARNING If you calibrate with the Syringepet measure measure m...

Page 52: ...emove the injection device you should see RUNNING RUNNING RUNNING RUNNING STIR ON STIR ON STIR ON STIR ON WASHING WASHING WASHING WASHING If the probe current is outside the expected limits you will see ERROR MEMB SENS ERROR MEMB SENS ERROR MEMB SENS ERROR MEMB SENS If you see this display press MENU then see Section 9 Troubleshooting If there is no problem the display will read WAIT WAIT WAIT WAI...

Page 53: ...RIME 1 PRIME 1 PRIME 1 PRIME 2 2 2 2 Check the linearity of the membrane by injecting a sample of either the YSI 2328 15 mmol or the YSI 1530 30 mmol lactate standard Press ENTER ENTER ENTER ENTER the display will read ENTER ID XXX ENTER ID XXX ENTER ID XXX ENTER ID XXX No ID is required so press ENTER ENTER ENTER ENTER the display will read WAIT WAIT WAIT WAIT INJECT SAMPLE INJECT SAMPLE INJECT S...

Page 54: ...NU and the display will read SAMPLE SAMPLE SAMPLE SAMPLE 0 0 0 0 CAL CAL CAL CAL 1 PRIME 1 PRIME 1 PRIME 1 PRIME 2 2 2 2 You are now ready to measure samples 6 2 Sample Cycle With the SPORT calibrated as described you can proceed to measure lactate values in blood samples or plasma WARNING WARNING WARNING WARNING If you calibrate with the Syringepet measure measure measure measure with the Syringe...

Page 55: ...will read ENTER ID XXX ENTER ID XXX ENTER ID XXX ENTER ID XXX When the ID display appears enter the ID as a three digit number example one is entered as 001 two hundred and thirteen as 213 then press ENTER ENTER ENTER ENTER and this display will appear WAIT WAIT WAIT WAIT If the probe current is not within the expected range you will see this display ERROR BASELINE ERROR BASELINE ERROR BASELINE ER...

Page 56: ...sult will be displayed along with the ID date and time Press MENU MENU MENU MENU to return to the Run menu The previous result ID date and time will be stored in memory until it is deleted SAMPLE SAMPLE SAMPLE SAMPLE 0 0 0 0 CAL CAL CAL CAL 1 PRIME 1 PRIME 1 PRIME 1 PRIME 2 2 2 2 6 3 Recalling Stored Results The SPORT allows you to recall any stored result Results may be recalled by ID by date or ...

Page 57: ... XXmmol L XXX X XXmmol L XXX X XXmmol L XXX X XXmmol L Every time you press ENTER ENTER ENTER ENTER the next value stored will be displayed You can cycle step by step through all of the results stored in memory The date time ID number will change as necessary to match each sample When all of the results have been displayed you will see this display NO SAMPLES NO SAMPLES NO SAMPLES NO SAMPLES PRESS...

Page 58: ...ill appear MM DD YY HH MM MM DD YY HH MM MM DD YY HH MM MM DD YY HH MM XXX X XXmmol L XXX X XXmmol L XXX X XXmmol L XXX X XXmmol L Every time you press ENTER ENTER ENTER ENTER the next value stored will be displayed You can cycle step by step through all of the results on this date The ID number will change as necessary to match each sample When all of the results have been displayed you will see ...

Page 59: ... will appear Key in a date as described in Section 3 6 System Setup then press ENTER ENTER ENTER ENTER Example January 17 2003 is entered as 01 17 03 ENTER 01 17 03 ENTER 01 17 03 ENTER 01 17 03 ENTER The first sample result stored in memory on that date will be displayed MM DD YY HH MM MM DD YY HH MM MM DD YY HH MM MM DD YY HH MM XXX X XXmmol L XXX X XXmmol L XXX X XXmmol L XXX X XXmmol L Every t...

Page 60: ...play press E E E ENTER NTER NTER NTER LIST ALL LIST ALL LIST ALL LIST ALL 0 0 0 0 BY DATE BY DATE BY DATE BY DATE 1 ID 1 ID 1 ID 1 ID 2 2 2 2 Select ID by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER ENTER ID XXX ENTER ID XXX ENTER ID XXX ENTER ID XXX Enter the desired ID as a three digit number from left to right then press ENTER ENTER ENTER ENTER The first sample result stored in memory that ha...

Page 61: ... RECALL 0 RECALL 0 RECALL 1 1 1 1 SETUP SETUP SETUP SETUP 2 DIAG 2 DIAG 2 DIAG 2 DIAG 3 3 3 3 Select Recall by pressing 1 1 1 1 then ENTER ENTER ENTER ENTER SAMPLES LCD SAMPLES LCD SAMPLES LCD SAMPLES LCD 0 0 0 0 PRINT PRINT PRINT PRINT 1 XMIT 1 XMIT 1 XMIT 1 XMIT 2 2 2 2 Select Print by pressing 1 1 1 1 then ENTER ENTER ENTER ENTER PRINT ALL PRINT ALL PRINT ALL PRINT ALL 0 0 0 0 BY DATE BY DATE B...

Page 62: ...ER ENTER PRINT ALL PRINT ALL PRINT ALL PRINT ALL 0 0 0 0 BY DATE BY DATE BY DATE BY DATE 1 ID 1 ID 1 ID 1 ID 2 2 2 2 Select Date by pressing 1 1 1 1 then ENTER ENTER ENTER ENTER DATE MM DD YY DATE MM DD YY DATE MM DD YY DATE MM DD YY XX XX XX XX XX XX XX XX XX XX XX XX NOTE NOTE NOTE NOTE The current date is what will appear on the display Press ENTER ENTER ENTER ENTER The display will be active d...

Page 63: ... date will appear Key in a date as described in Section 3 6 System Setup then press ENTER ENTER ENTER ENTER Example January 17 1998 is entered as 01 17 98 ENTER 01 17 98 ENTER 01 17 98 ENTER 01 17 98 ENTER The display will be active during the printing process showing each result as it is printed It is not possible to halt the printing once it has started It is not possible to halt the printing on...

Page 64: ...to halt the printing once it has started It is not possible to halt the printing once it has started It is not possible to halt the printing once it has started Once finished the display will return to the RECALL menu SAMPLES LCD SAMPLES LCD SAMPLES LCD SAMPLES LCD 0 0 0 0 PRINT PRINT PRINT PRINT 1 XMIT 1 XMIT 1 XMIT 1 XMIT 2 2 2 2 Use the MENU MENU MENU MENU key to return to the Main menu 6 5 Tra...

Page 65: ...o longer required This method requires that the result that is to be deleted be recalled on the display to make sure that the correct result is deleted The second method deletes all results Editing Results Editing Results Editing Results Editing Results Go to the Main menu by pressing MENU MENU MENU MENU until the display reads RUN RUN RUN RUN 0 RECALL 0 RECALL 0 RECALL 0 RECALL 1 1 1 1 SETUP SETU...

Page 66: ...D SAMPLES LCD SAMPLES LCD SAMPLES LCD 0 0 0 0 PRINT PRINT PRINT PRINT 1 XMIT 1 XMIT 1 XMIT 1 XMIT 2 2 2 2 To select viewing the results on the display press ENTER ENTER ENTER ENTER DISPLAY DISPLAY DISPLAY DISPLAY 0 0 0 0 DELETE ALL DELETE ALL DELETE ALL DELETE ALL 1 1 1 1 To delete all of the stored results press 1 1 1 1 then ENTER ENTER ENTER ENTER ARE YOU SURE ARE YOU SURE ARE YOU SURE ARE YOU S...

Page 67: ...packaged in your starter kit and may also be ordered separately With the instrument in SAMPLE mode inject 25 microliters of the solution with whichever injection device was used for calibration The reading should be below 0 3 mmol L 2 7 mg dL If it is greater repeat the test If it s still too high change the membrane Linearity Test Linearity Test Linearity Test Linearity Test Use YSI 1530 30 mmol ...

Page 68: ...6 6 6 6 22 22 22 22 ...

Page 69: ...er the infrared data link Keypad Each key can be verified for performance Display A diagnostic routine checks every LCD segment for failure Battery Battery voltage can be monitored on the display 7 1 Checking Probe Current Go to Main menu by pressing MENU MENU MENU MENU until you see RUN RUN RUN RUN 0 RECALL 0 RECALL 0 RECALL 0 RECALL 1 1 1 1 SETUP SETUP SETUP SETUP 2 DIAG 2 DIAG 2 DIAG 2 DIAG 3 3...

Page 70: ... X XX nA PROBE X XX nA PROBE X XX nA PROBE X XX nA The display will update every few seconds To leave this mode press MENU MENU MENU MENU PROBE PROBE PROBE PROBE 0 0 0 0 R R R REF EF EF EF 1 BUF 1 BUF 1 BUF 1 BUF 2 2 2 2 Press MENU MENU MENU MENU until main menu appears 7 2 Checking Sample Chamber Temperature Go to Main menu by pressing MENU MENU MENU MENU until you see RUN RUN RUN RUN 0 RECALL 0 ...

Page 71: ... TEMP 0 TEMP 1 1 1 1 INJ SW INJ SW INJ SW INJ SW 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select Injection Switch by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER INJECTION SWITCH INJECTION SWITCH INJECTION SWITCH INJECTION SWITCH OFF OFF OFF OFF Inserting an injection device will trigger the switch and the change in state will be indicated ON OFF on the display Press MENU MENU MENU MENU to return to t...

Page 72: ...RECALL 1 1 1 1 SETUP SETUP SETUP SETUP 2 DIAG 2 DIAG 2 DIAG 2 DIAG 3 3 3 3 Select Diagnostics by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER PROBE PROBE PROBE PROBE 0 TEMP 0 TEMP 0 TEMP 0 TEMP 1 1 1 1 INJ SW INJ SW INJ SW INJ SW 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select More by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER STIR STIR STIR STIR 0 PUMPS 0 PUMPS 0 PUMPS 0 PUMPS 1 1 1 1 PRINT PRINT ...

Page 73: ...PS 0 PUMPS 1 1 1 1 PRINT PRINT PRINT PRINT 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select Print by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER TEST PRINT TEST PRINT TEST PRINT TEST PRINT 0 0 0 0 TEST RS232 TEST RS232 TEST RS232 TEST RS232 1 1 1 1 Press ENTER ENTER ENTER ENTER to send a test form over the infrared data link PRINTING PRINTING PRINTING PRINTING TEST PRINT TEST PRINT TEST PRINT TEST PRI...

Page 74: ...R ENTER ENTER ENTER to send a test data string over the RS232 data link XMITTING XMITTING XMITTING XMITTING XXXXXXXXXXXXXX XXXXXXXXXXXXXX XXXXXXXXXXXXXX XXXXXXXXXXXXXX TEST PRINT TEST PRINT TEST PRINT TEST PRINT 0 0 0 0 TEST RS232 TEST RS232 TEST RS232 TEST RS232 1 1 1 1 Press MENU MENU MENU MENU to return to the Main menu 7 8 Testing the Keypad Go to Main menu by pressing MENU MENU MENU MENU unti...

Page 75: ...UP SETUP SETUP SETUP 2 DIAG 2 DIAG 2 DIAG 2 DIAG 3 3 3 3 Select Diagnostics by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER PROBE PROBE PROBE PROBE 0 TEMP 0 TEMP 0 TEMP 0 TEMP 1 1 1 1 INJ SW INJ SW INJ SW INJ SW 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select More by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER STIR STIR STIR STIR 0 PUMPS 0 PUMPS 0 PUMPS 0 PUMPS 1 1 1 1 PRINT PRINT PRINT PRINT 2 MORE...

Page 76: ... PUMPS 1 1 1 1 PRINT PRINT PRINT PRINT 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select More by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER KEYS KEYS KEYS KEYS 0 DIS 0 DIS 0 DIS 0 DISPLAY PLAY PLAY PLAY 1 1 1 1 VER VER VER VER 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select Version by pressing 2 2 2 2 then ENTER ENTER ENTER ENTER The software version number will be displayed VERSION X XX LAC VERSION X XX L...

Page 77: ...AY 0 DISPLAY 1 1 1 1 VER VER VER VER 2 MORE 2 MORE 2 MORE 2 MORE 3 3 3 3 Select More by pressing 3 3 3 3 then ENTER ENTER ENTER ENTER BATTERY BATTERY BATTERY BATTERY 0 0 0 0 Press ENTER ENTER ENTER ENTER to display the non non non non loaded loaded loaded loaded battery voltage BATTERY XX XX BATTERY XX XX BATTERY XX XX BATTERY XX XX XX XX XX XX The display will update every few seconds Press MENU ...

Page 78: ...7 7 7 7 10 10 10 10 ...

Page 79: ...Levels Check Buffer and Reference Bottle Levels Check Buffer and Reference Bottle Levels Check Buffer and Reference Bottle Levels The SPORT does not sense fluid levels automatically so you have to look to see that you have enough If you run out and don t realize it you ll get wrong data and you may lose samples Replace the Buffer and Reference solutions if they are low or have been in the instrume...

Page 80: ...tarter Kit should be cleaned every week with a disinfecting agent The bottles and Syringe should be rinsed completely with distilled water after cleaning to remove any disinfecting residue which would cause membrane failure if it got into the system 8 3 Cleaning the Sample Chamber Clean the sample chamber every month Unscrew the probes from their housings If the membrane has passed the tests in Se...

Page 81: ...ntenance Sensor Maintenance Sensor Maintenance Sensor Maintenance It is necessary to maintenance the enzyme sensor when the 1503 PM kit is installed and periodically as needed 1 Remove the enzyme membrane and hold the probe with the electrodes facing up 2 Wad a small portion of a lint free tissue and wet it with 70 isopropyl alcohol 3 Using your thumb press the alcohol soaked wad against the probe...

Page 82: ...nsor in the solution for about 45 seconds 5 Remove the sensor and immediately rinse it with reagent grade water for at least 2 minutes 6 Install a new membrane and return the probe to service NOTE NOTE NOTE NOTE Several hours may be required for the sensor to stabilize after cleaning 8 6 Tubing Replacement The buffer and reference solutions are moved through the SPORT with peristaltic tubing pumps...

Page 83: ...y pump tubing size 5 32 OD x 1 32 wall Supply pump tubing size 5 32 OD x 1 32 wall Supply pump tubing size 5 32 OD x 1 32 wall Supply pump tubing size 5 32 OD x 1 32 wall Waste pump tubing size 3 16 OD x 1 32 wall Waste pump tubing size 3 16 OD x 1 32 wall Waste pump tubing size 3 16 OD x 1 32 wall Waste pump tubing size 3 16 OD x 1 32 wall Roller Body Make sure tubing is not twisted inside body b...

Page 84: ...l flicker on and off rapidly This is the indication that the charger is floating An overnight charge should be more than adequate to insure a full day of battery only testing the next day The SPORT may be turned back on immediately or left with the power off The battery will be recharged in either condition Turning on the power will allow immediate continuation once the probe has restabilized WARN...

Page 85: ...ly by first emptying all of the fluid bottles and then rinsing and filling the supply bottles with distilled or deionized water The system can then be either cycled using the priming function available in RUN mode or it may be done through DIAGNOSTICS using the individual functions Storage of the unit for less than a week can be done with the buffer and reference fluids in place NOTE NOTE NOTE NOT...

Page 86: ......

Page 87: ...psed times with the recommended times Look and listen for problems unusually noisy components loose connections fluid leaks air bubbles in the sample chamber salt build ups etc Review Section 7 Instrument Diagnostics to become familiar with testing individual components of the SPORT Use the troubleshooting chart in this section to assist you in identifying the problem then use the chart to guide y...

Page 88: ... Customer Service as this is a system failure that may require returning the instrument The second option is to select YES which will reformat the SPORT memory The software will attempt to correct itself but in doing so ALL stored data will be lost If YES was selected the stir speed date and time must also be reset Refer to Section 3 Instrument Setup for more information if necessary IMPORTANT IMP...

Page 89: ...this point one is to select NO and save your data and decide not to continue Choosing this option will display the following POWER DOWN POWER DOWN POWER DOWN POWER DOWN CONSULT MANUAL CONSULT MANUAL CONSULT MANUAL CONSULT MANUAL We recommend at this point that you contact YSI Customer Service as this is a system failure that may require returning the instrument The second option is to select YES w...

Page 90: ...llow you to continue sampling though until the battery has been recharged to a level of 12 8 volts This is done to protect the discharge recharge capacity of the battery and insure the maximum amount of good usable battery life Enzyme Sensor Faults Enzyme Sensor Faults Enzyme Sensor Faults Enzyme Sensor Faults ERROR BASELINE ERROR BASELINE ERROR BASELINE ERROR BASELINE PRESS MENU PRESS MENU PRESS ...

Page 91: ...CORRECTIVE ACTION Enter PUMP diagnostics and verify buffer pump performance SECTION 7 5 POSSIBLE CAUSE Buffer pump not performing properly CORRECTIVE ACTION Replace tubing SECTION 8 6 POSSIBLE CAUSE Stir bar not present CORRECTIVE ACTION Disassemble chamber and reinstall stir bar SECTION 8 3 POSSIBLE CAUSE Stir bar not spinning CORRECTIVE ACTION Enter STIR diagnostics and check motor performance S...

Page 92: ...Clean probe surface SECTION 8 5 POSSIBLE CAUSE Main power disruption CORRECTIVE ACTION Enter PROBE diagnostics and check probe current SECTION 7 1 ERROR MEMB SENS ERROR MEMB SENS ERROR MEMB SENS ERROR MEMB SENS PRESS MENU PRESS MENU PRESS MENU PRESS MENU This fault is generated only during the Calibration cycle Because a sample of a known concentration 5 mmol L Lactate Standard has been injected t...

Page 93: ...tor solution and repeat procedure POSSIBLE CAUSE Enzyme probe surface fouled CORRECTIVE ACTION Clean probe surface SECTION 8 5 POSSIBLE CAUSE Temperature probe auxiliary electrode surface fouled CORRECTIVE ACTION Clean probe surface SECTION 8 5 POSSIBLE CAUSE Stir bar not present CORRECTIVE ACTION Disassemble chamber and reinstall stir bar SECTION 8 3 POSSIBLE CAUSE Stir bar not spinning CORRECTIV...

Page 94: ... TEMP ERR BAD TEMP ERR BAD TEMP ERR BAD TEMP PRESS MENU PRESS MENU PRESS MENU PRESS MENU The instrument will work only over the specified temperature range 5 45 C POSSIBLE CAUSE Ambient temperature too cold or hot CORRECTIVE ACTION Operate at ambient temperatures between 5 and 45 C TEMP UNSTABLE PRESS TEMP UNSTABLE PRESS TEMP UNSTABLE PRESS TEMP UNSTABLE PRESS MENU MENU MENU MENU POSSIBLE CAUSE If...

Page 95: ...ive effect on the performance of the SPORT at the upper most part of the dynamic range If this fault is encountered during an attempt to calibrate the instrument replace the Reference solution and repeat the calibration process ERROR REF ERROR REF ERROR REF ERROR REF CONCENTRATION HI CONCENTRATION HI CONCENTRATION HI CONCENTRATION HI This warning is generated when the Reference solution concentrat...

Page 96: ...then re calibrate SECTION 7 1 POSSIBLE CAUSE Supply tubing crimped prohibiting reference solution flow to the chamber CORRECTIVE ACTION Visually inspect all pump tubing 9 2 Possible Failure Conditions The following are possible failures that may go undetected unless confirmed visually but can affect instrument performance Air bubble in the sample chamber Air bubble in the sample chamber Air bubble...

Page 97: ... Reference pump fail to pump fluid Buffer and or Reference pump fail to pump fluid Buffer and or Reference pump fail to pump fluid Buffer and or Reference pump fail to pump fluid POSSIBLE CAUSE Tubing line in pumps crimped or stuck shut CORRECTIVE ACTION Disassemble pumps and open tubing or replace if more than 6 months since last replaced SECTION 8 6 POSSIBLE CAUSE Supply line blocked CORRECTIVE ...

Page 98: ...ORRECTIVE ACTION Flush check valve or replace POSSIBLE CAUSE Bottle vent plugged causing positive pressure in the waste bottle CORRECTIVE ACTION Loosen waste bottle cap to verify clear blockage or replace vent POSSIBLE CAUSE Capillary tube plug broken loose from tube and stuck in waste chamber port CORRECTIVE ACTION Disassemble chamber check for blockage in outlet port upper SECTION 8 3 POSSIBLE C...

Page 99: ...9 9 9 9 13 13 13 13 POSSIBLE CAUSE Stir motor failure CORRECTIVE ACTION Go to stir motor diagnostics and verify SECTION 7 4 ...

Page 100: ...9 9 9 9 14 14 14 14 ...

Page 101: ...0 18 0 22 0 26 0 30 0 YSI 1500 vs 2300 YSI 2300 mmol l WHOLE BLOOD CAPILLARY 0 25 TX 100 LACTATE 30 0 24 0 22 0 20 0 18 0 16 0 14 0 12 0 10 0 8 0 6 0 4 0 2 0 0 0 Y 0 9973X 0705 DATA 26 0 28 0 1500 SPORT mmol l Total Blood Lactate YSI 1515 cell lysing agent added to the buffer solution in both instruments Concentration Concentration Concentration Concentration Pooled Est Std Dev Pooled Est Std Dev ...

Page 102: ...0 26 0 24 0 22 0 20 0 18 0 16 0 14 0 12 0 10 0 8 0 6 0 4 0 2 0 0 0 Y 1 0095X 0224 DATA Whole Blood Lactate Extracellular ONLY Concentration Concentration Concentration Concentration Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Mean Bias Mean Bias Mean Bias Mean Bias 0 10 mmol L 0 1 mmol L 0 1 mmol L 10 20 0 3 0 2 20 30 0 4 0 3 ...

Page 103: ...WHOLE BLOOD SYRINGEPET 0 25 TX 100 LACTATE 30 0 26 0 24 0 22 0 20 0 18 0 16 0 14 0 12 0 10 0 8 0 6 0 4 0 2 0 0 0 Y 1 0078X 0714 DATA 28 0 Total Blood Lactate YSI 1515 cell lysing agent added to the buffer solution in both instruments Concentration Concentration Concentration Concentration Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Mean Bias Mean Bias Mean Bias Mean...

Page 104: ...0 24 0 22 0 20 0 18 0 16 0 14 0 12 0 10 0 8 0 6 0 4 0 2 0 0 0 Y 1 0076X 1302 DATA 28 0 Whole Blood Lactate Extracellular ONLY Concentration Concentration Concentration Concentration Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Mean Bias Mean Bias Mean Bias Mean Bias 0 10 mmol L 0 1 mmol L 0 1 mmol L 10 20 0 3 0 2 20 30 0 4 0 3 ...

Page 105: ...16 0 14 0 12 0 10 0 8 0 6 0 4 0 2 0 0 0 Y 0 9793X 1345 DATA Plasma Lactate YSI 1515 cell lysing agent added to the buffer solution in both instruments Concentration Concentration Concentration Concentration Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Mean Bias Mean Bias Mean Bias Mean Bias 0 10 mmol L 0 0 mmol L 0 0 mmol L 10 20 0 1 0 1 20 30 0 4 0 4 ...

Page 106: ... 0 0 Y 0 9893X 1559 DATA 26 0 28 0 1500 SPORT mmol l Plasma Lactate Without YSI 1515 cell lysing agent added to the buffer solution of either instrument Concen Concen Concen Concentration tration tration tration Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Pooled Est Std Dev Mean Bias Mean Bias Mean Bias Mean Bias 0 10 mmol L 0 1 mmol L 0 1 mmol L 10 20 0 2 0 1 20 30 0 3 0 1 ...

Page 107: ...ibit enzyme activity and prevent bacterial growth Separate the red blood cells by centrifugation within 30 minutes of collection If delays between collection and assay are unavoidable follow one of these procedures 1 Refrigerate the whole blood at 4 C This is acceptable for periods up to four hours 2 Or refrigerate the plasma with preservatives at 4 C for up to three days Plasma with preservative ...

Page 108: ... tube with a sterile glass rod or applicator Add sterile beads if desired Centrifuge the specimen promptly to separate the serum from the clot If delays are unavoidable follow one these procedures 1 Refrigerate the whole blood at 4 C This is acceptable for periods of up to 4 hours 2 Or prolonged storage isolate the serum from the clot within 30 minutes of collection and freeze it at 10 C or colder...

Page 109: ...ncentration of 2 5mg fluoride and 1 mg EDTA per ml of blood These are not know to cause any error in the measurement of whole blood lactate but they may cause a reduction of the sample s hematocrit value with or without accompanying hemolysis This would lead to a slight error in the calculated plasma lactate value Hemolysis can cause disagreement between readings on the Model 1500 and some referen...

Page 110: ...an an hour particularly if they have been refrigerated should be placed on a blood rotator for five minutes to break up clumps of cells Air bubbles or froth in the sample drawn into the sipper can lead to significant errors in the form of low lactate readings Because it is difficult to detect bubbles visually in whole blood specimens you should exercise great care Any unexpectedly low lactate read...

Page 111: ...actate does not however due to the time required to diffuse through the cell walls Given sufficient time the lactate would diffuse through the cell walls and then dilute into the buffer solution allowing all of the the lactate to be measured This is not practical however as the rate at which this diffusion would take place would severely lengthen the duration of each measurement cycle Therefore th...

Page 112: ...reparation process explained in Section 3 3 Reagent Preparation Buffer Solution Buffer Solution Buffer Solution Buffer Solution Fill the 500mL mixing bottle with reagent water Reagent water must be very pure Use high quality deionized water or distilled water Add one package of YSI 2357 Buffer Concentrate and stir waiting long enough to be certain that the buffer chemicals have completely dissolve...

Page 113: ...3 3 3 WARNING If you switch from running with to running without the lysing agent or vice versa you cannot get reliable results unless you allow sufficient time for complete removal of the agent from the fluid system ...

Page 114: ...12 12 12 12 4 4 4 4 ...

Page 115: ...ervative tubes unless the lysing agent is used in the instrument when the actual measurement takes place This is due to the fact that the preservative tubes contain a detergent This detergent when introduced into a system without the cell lysing agent can affect the membrane response and cause incorrect readings While this detergent is also present in the cell lysing agent the response effect is c...

Page 116: ...13 13 13 13 2 2 2 2 ...

Page 117: ...stem or cause erroneous readings Most of these have no place in diagnostic medicines but may be of scientific interest Some substances such as triglycerides which are interferences for photometric sensor systems do not interfere with the SPORT electrochemical sensor system Reducing Agents Reducing Agents Reducing Agents Reducing Agents Many reducing agents would give rise to a false signal current...

Page 118: ... Azide Calibration Shift by Detergents Calibration Shift by Detergents Calibration Shift by Detergents Calibration Shift by Detergents The sensitivity of the platinum anode of the sensor is affected somewhat by absorption or desorption of material from the buffer which bathes it In normal operation such changes are quite gradual and are corrected in the periodic recalibration of the instrument How...

Page 119: ... now drugs that are abused that is ingested at levels much higher than the usually recommended therapeutic doses In patients with higher than therapeutic levels there is hazard of gross masking of lactate concentration by the interfering substance The SPORT should not be used to analyze specimens containing any of these substances at or above the listed Interfering Level Physical Damage Physical D...

Page 120: ...utyric Acid Sodium salt 126 4 Hydroxybutyric Acid Sodium salt 126 09 DL Malic Acid 134 09 DL a Hydroxybutyric Acid 126 09 84 6 7 L B Phenyllactic Acid 166 18 24 1 4 B Chlorolactic Acid 124 52 93 2 4 Oxalacetic Acid 132 07 Glycine 75 07 DL Alanine 89 09 B Alanine 89 09 DL a Hydroxycaproic Acid 132 16 45 3 4 Pyruvic Acid Sodium Salt 110 04 DL a Hydroxyisovaleric Acid 118 13 L a Hydroxyisocaprioc Aci...

Page 121: ...actose 180 16 D Glucose 180 16 D Glucosamine HC1 215 64 Glucose 6 phosphate Monosodium 282 12 Uric Acid 168 11 p Aminosalicylic Acid 153 14 8 0 0 52 2 3 Dimercapto propanol 124 22 Guaiacol 124 14 3 1 0 25 Hydrazine Sulfate 130 12 13 1 0 Hydroquinone 110 11 4 3 0 39 Hydroxylamine Hydrochloride 69 49 1 6 0 23 Isoniazid 137 14 12 0 88 2 Mercaptoethanol 78 13 1 0 0 13 p Phenylenediamine HC1 181 07 2 9...

Page 122: ...14 14 14 14 6 6 6 6 ...

Page 123: ...ply bottles If this happens it is quite possible that the 1500 SPORT will detect TEMPERATURE STABILITY ERRORS Simply keeping the unit in the shade regardless of ambient temperature will assure that the warming or cooling effects are consistent and therefore the results accurate Temperature Effect On Battery Life Temperature Effect On Battery Life Temperature Effect On Battery Life Temperature Effe...

Page 124: ...15 15 15 15 2 2 2 2 ...

Page 125: ...Membranes 1 2363 Potassium Ferrocyanide 1 2392 NaCl Solution 1 2357 Buffer Concentrate Kit 1 1505 Capillary Tubes 1 1515 Cell Lysing Agent 1 Syringe 3cc 1 1505 25 microliter Capillary Tube Package 100 count 1506 Hewlet Packard Infrared Printer HP 82240 1515 Cell Lysing Agent 8 packs 1530 Lactate Standard 30 mmol L 125mL 015117 120 VAC 60 Hz Battery Charger 015118 220 VAC 50 Hz Battery Charger 2315...

Page 126: ...16 16 16 16 2 2 2 2 ...

Page 127: ... cleaning and disinfecting solutions Suggested cleaning and disinfecting solutions Isopropanol 70 Sodium hypochlorite 5000 ppm free available chlorine 1 10 solution of house hold bleach Disassemble parts that require cleaning per instructions in Section 8 Maintenance Thoroughly clean with the disinfecting agent Rinse with warm water WARN WARN WARN WARNING ING ING ING Clean probes with isopropanol ...

Page 128: ...17 17 17 17 2 2 2 2 ...

Page 129: ... you of defective or improper components or parts in servicing or repairing the product or vi modification of the product in any way not expressly authorized by YSI THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT ...

Page 130: ... if in our opinion it represents a potential biological or radioactive hazard our service personnel reserve the right to withhold service until appropriate cleaning decontamination and certification has been completed We will contact the sender for instructions as to the disposition of the equipment Disposition costs will be the responsibility of the sender When service is required either at the u...

Page 131: ... out dirt and packing material 4 Use a large carton preferably the original and surround the product completely with packing material 5 Insure for the replacement value of the product Cleaning Certificate Organization Department Address City State Zip Country Phone Model No of Device Lot Number Contaminant if known Cleaning Agent s used Radioactive Decontamination Certified Answer only if there ha...

Page 132: ...18 18 18 18 4 4 4 4 ...

Page 133: ...icular installation If this equipment does cause interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures reorient the receiving antenna relocate the computer with respect to the receiver move the computer away from the receiver plug the computer into ...

Page 134: ...num Lane Yellow Springs Ohio 45387 USA 937 767 7241 800 659 8895 Fax 937 767 8058 Info ysi com www YSI com CEPartner4U BV Esdoornlaan 13 3951DB Maarn The Netherlands 31 0 6 516 536 26 015162 A15162H 2003 12 2003 YSI Incorporated ...

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