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50,000 counts to allow for negative baseline offsets in the 200 cm cell caused by
sample salinity.
Note:
Experiments have shown that three to five cleaning cycles were sufficient to clean the cell or
organic contamination. In extreme cases (for example, CDOM left in the UltraPath 2 cell
overnight), it was found that filling the waveguide with Solution 1 and letting it sit for several
minutes (
e.g.
, 10 minutes) and then subsequently flushing with Solutions 2 and 3 were
necessary to remove contamination at the cell wall.
All solvents used should be HPLC grade.
