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Using UltraPath 2
UltraPath 2 is a very sensitive device for measuring low absorbing substances in solution.
The following instructions will familiarize you with using and cleaning the UltraPath 2 system.
•
Switch the TIDAS S 300 UV/VIS ON (10) and start the TIDASDAQ software. Allow its
light source approximately 15 minutes warming up time for the lamp to stabilize its
temperature drift (<0.5mAU/h). For test purposes, the lamp may be used earlier.
Open the shutter (14) at the light source to take measurements. Close the shutter
when a dark current has to be taken.
•
Switch the pump (Peri-Star Pro) ON (22) and with size #14 tubing set the pump speed
to 60 RPM or 12mL/min via the control panel (21). The sample is drawn into the
waveguide by vacuum suction.
•
Select a pathlength (10, 50, 200 cm) at the fiber optic switch (4) of the UltraPath 2
sample cell.
How to fill the UltraPath 2 sample cell
•
Place the injector (5) into a sample vial. The sample is drawn with the peristaltic
pump (Peri-Star Pro) into the UltraPath 2 sample cell by vacuum suction.
•
Press the forward button at the control panel (21) of the Peri-Star Pro to start
drawing sample solution into the cell.
•
Lift the sample injector between samples to allow for an air bubble to enter the
injector tubing. In this way samples can be separated and cross contamination due to
mixing minimized.
•
Using the 10cm or 50cm cell, a pumping time of approx. 30 seconds is necessary,
using the 200cm path, a pumping time of approximately 60 seconds is necessary to
fill the cell completely with sample solution.
•
The cell is completely filled when the air bubble generated earlier exits the “Liquid
out” connector (3) of the sample cell.
