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Summary of Contents for ACQUITY UPLC I-Class

Page 1: ...ACQUITY UPLC I Class System Guide 715003736 Revision B Copyright Waters Corporation 2014 All rights reserved...

Page 2: ...Waters Web site waters com Trademarks ACQUITY ACQUITY UPLC Connections INSIGHT Millennium PIC UPLC and Waters are registered trademarks and Auto Blend Plus eCord Empower LAC E MassLynx XBridge and THE...

Page 3: ...reach us at tech_comm waters com Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use transportation removal or disposal of any Waters product You can r...

Page 4: ...Class instruments See also For safety considerations regarding specific system modules consult the appropriate information on the user documentation CD High voltage hazard Safety advisories Consult Ap...

Page 5: ...applications Calibrating To calibrate LC systems follow acceptable calibration methods using at least five standards to generate a standard curve The concentration range for standards should include t...

Page 6: ...ion from day to day and run to run Data collected when QC samples are out of range might not be valid Do not report these data until you are certain that the instrument performs satisfactorily ISM cla...

Page 7: ...vii EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX United Kingdom Telephone 44 161 946 2400 Fax 44 161 946 2480 Contact Quality manager...

Page 8: ...viii...

Page 9: ...ntended use of the ACQUITY UPLC I Class system v Calibrating v Quality control vi ISM classification vi ISM Classification ISM Group 1 Class B vi EC authorized representative vii 1 ACQUITY UPLC I Clas...

Page 10: ...1 11 2 Optimizing Performance 2 1 General guidelines 2 2 Dispersion 2 3 Carryover 2 4 Contamination 2 5 Reproducibility 2 5 Cycle time between injections 2 5 Preventing leaks 2 5 Sample preparation 2...

Page 11: ...2 Task specific hazard warnings A 2 Specific warnings A 3 Caution advisory A 5 Warnings that apply to all Waters instruments A 5 Electrical and handling symbols A 11 Electrical symbols A 11 Handling s...

Page 12: ...xii Table of Contents...

Page 13: ...for optimum performance when running difficult assays Configure it with a mass spectrometer to take full advantage of the system s design enhancements Contents Topic Page UltraPerformance Liquid Chro...

Page 14: ...are as high as 18 000 psi The van Deemter equation an empirical formula that describes the relationship between linear velocity and column efficiency considers particle size as one of the variables Th...

Page 15: ...matographic conditions for the separations were identical except for the flow rate which was scaled according to particle size Features of the ACQUITY UPLC I Class system The ACQUTY UPLC I Class syste...

Page 16: ...maintaining the integrity of the separation Column compartments Column compartments configured with I Class systems ensure reliable and robust separations This is especially important when you transfe...

Page 17: ...ometry applications Detectors The PDA and TUV detectors available with the I Class system feature low dispersion UV flow cell technology which enhances peak resolution Mass spectrometry When you confi...

Page 18: ...ugh needle or fixed loop a column heater CH A detectors tunable ultraviolet photodiode array e photodiode array or mass spectrometry and an ACQUITY UPLC column Waters Empower chromatography data softw...

Page 19: ...s Sample Manager FTN uses a flow through needle mechanism that differs from a loop based injector The mechanism aspirates a sample from plates and vials and holds it in the sample needle in preparatio...

Page 20: ...ACQUITY UPLC I Class system s column heater helps to ensure precise reproducible separations by controlling the column temperature The column heater CH A heats the column compartment to any temperatu...

Page 21: ...UPLC Systems with 2D Technology Capabilities Guide Sample organizer optional Requirement Verify that the sample organizer you use with your I Class system is compatible with sample managers fitted wi...

Page 22: ...t pressure that the column has experienced and the date The highest temperature the column has experienced and the date In addition to the variable column usage data the eCord column chip also stores...

Page 23: ...ou can find this additional information about the ACQUITY I Class system ACQUITY UPLC Binary Solvent Manager Operator s Overview and Maintenance Information ACQUITY UPLC Sample Manager Flow Through Ne...

Page 24: ...1 12 ACQUITY UPLC I Class System...

Page 25: ...chapter to help ensure optimum performance from your ACQUITY UPLC I Class system Contents Topic Page General guidelines 2 2 Dispersion 2 3 Carryover 2 4 Contamination 2 5 Reproducibility 2 5 Cycle ti...

Page 26: ...less than 0 5 seconds wide Waters recommends a sampling rate of 25 to 50 points across a peak which provides good quantitation Sampling rates faster than 20 points per second yield higher baseline noi...

Page 27: ...nt lines during system startup Keep all solvent lines primed Monitor the waste level to ensure that it is never too high When starting the binary solvent manager ensure solvent flows through pump A an...

Page 28: ...analyte remains in the system after a sample is injected You can measure carryover by observing analyte peaks that appear when you run a blank sample immediately after an analytical sample See ACQUIT...

Page 29: ...pecifications for details Cycle time between injections The short run time of a UPLC separation requires efficient use of the time between analyses The ACQUITY SM FTN and SM FL have Load Ahead and Loo...

Page 30: ...nnecting active preheaters Sample preparation UPLC analysis places some additional restrictions on sample preparation Particulates The small column frit size 0 2 m can become blocked more easily than...

Page 31: ...3 1 3 Preparing the System Contents Topic Page Preparing system hardware 3 2 Configuring chromatography data software 3 5 ACQUITY control panels 3 6 Starting the ACQUITY UPLC Console 3 13...

Page 32: ...le manager by pressing the power switch on the top left hand side of each devices door See also Status LEDs on page 3 3 and Power LED on page 3 3 for information on how to interpret LED modes for devi...

Page 33: ...de of the power LED on the binary solvent manager s front panel indicates the flow status A steady green flow LED indicates flow through the binary solvent manager Run LED sample manager The run LED o...

Page 34: ...plete any outstanding samples or diagnostic function requests When sample and diagnostic function requests are finished the LED reverts to the unlit mode Detector Indicates the detector lamp is ignite...

Page 35: ...nt when you intend to use fresh solvents of the same composition as those already in the system To refresh the system 1 In the ACQUITY UPLC Console click Control Start up system 2 In the Start up syst...

Page 36: ...ol panel displays flow status system pressure total flow rate and solvent composition parameters Rule You can edit these parameters when the system is idle by clicking the underlined value You cannot...

Page 37: ...top Flow Immediately stops all flow from the binary solvent manager Additional functions in the binary solvent manager control panel Control panel function Description Start up system Brings the syste...

Page 38: ...ulating air whenever the sample compartment door is open Wash plungers Initiates the plunger wash sequence which fills and then slowly empties the primary and accumulator chambers with the current sol...

Page 39: ...ed Sample Compartment Set Point Displays the current sample compartment set point to 0 1 C resolution When active temperature control is disabled this field displays Off Current Column Heater Temperat...

Page 40: ...Photodiode Array Detector Getting Started Guide Control panel Additional functions in the sample manager control panel Control panel function Description Prime Displays the Prime dialog box See Primin...

Page 41: ...dow TUV detector control panel items Control panel item Description Lamp On Off LED Displays the actual lamp on off LED on the front panel of the detector unless communications with the detector are l...

Page 42: ...ctions by right clicking anywhere in the column manager control panel Column manager control panel items Control panel item Description Run LED Displays the actual run LED on the front panel of the co...

Page 43: ...ations of each module and its components You can also navigate to interactive diagrams which show interconnections and provide diagnostic tools for troubleshooting problems To start the ACQUITY UPLC C...

Page 44: ...3 14 Preparing the System...

Page 45: ...es also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply to...

Page 46: ...electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the specific risk and explains ho...

Page 47: ...tion Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran THF and nitric or sulfuric ac...

Page 48: ...al surfaces of the mass spectrometer when the instrument is in Operate mode To avoid nonlethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high vo...

Page 49: ...this device follow standard quality control procedures and the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous substances To avo...

Page 50: ...er Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren Avv...

Page 51: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...

Page 52: ...iamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi n El usuari...

Page 53: ...Warnings that apply to all Waters instruments A 9 Nonmetallic Tetrahydrofuran THF Methylene chloride Dimethyl sulfoxide THF...

Page 54: ...tueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden nicht ordnungsgem funktionieren Atte...

Page 55: ...ls These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor ter...

Page 56: ...visories Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry Fragil...

Page 57: ...m modules If you must transport a system component or remove it from service contact Waters Technical Service for recommended cleaning flushing and packaging procedures System tubing connections The s...

Page 58: ...ample Manager FTN BSM solvents SM wash solvent Column Column heater or manager APH Needle Sample organizer Injection port To waste Injector valve Vent valve Mixer Waste Pump A Pump B Binary solvent ma...

Page 59: ...r FL SM wash solvents Column Column heater or manager APH Needle Sample organizer Wash port To waste Sample syringe Injector valve Vent valve Mixer Waste Pump A Pump B Binary solvent manager Degasser...

Page 60: ...ar panel connections for ACQUITY UPLC I Class system modules are shown below TP03423 Ethernet switch External power and communications cable CH A and CH 30A only Ethernet cables Power cords Ethernet c...

Page 61: ...lumn heater and the sample manager To make column heater connections 1 Make sure the sample manager and the column heater are powered off 2 Connect the external communication cable to the High Density...

Page 62: ...nector Signal cable To make signal connections 1 Insert the connector into the connector port on the back of the system module 2 Using the flat blade screwdriver attach the positive and negative leads...

Page 63: ...h nut driver to tighten the locking nut until the fork terminal does not move Binary solvent manager I O signal connectors The rear panel of the binary solvent manager includes two removable connector...

Page 64: ...nary solvent manager has ceased operation because of an error condition or operator request Switch 1 Out Used to send time based contact closure signals to external devices 1 2 3 4 5 6 7 8 9 10 11 12...

Page 65: ...from the binary solvent manager when an error condition or hardware failure occurs on another system module Switch 2 Out Used to send time based contact closure signals to external devices Switch 3 Ou...

Page 66: ...to trigger a mass spectrometer or an ACQUITY 2996 PDA detector running under MassLynx software control to start Sample manager I O signal connectors For electrical specifications see the ACQUITY UPLC...

Page 67: ...em To connect to the electricity source Recommendation Use a line conditioner and uninterruptible power supply UPS for optimum long term input voltage stability Sample manager event out event in conne...

Page 68: ...n the rear panel of each system module Connect the hooded male end of the FlexCart s electrical cables to the power strips on the back of the cart Finally connect each power strip s cable to a wall ou...

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