Waters ACQUITY UPLC I-Class, System Manual, Page: 19 / 68

System modules 1-7
Binary solvent manager
The Waters ACQUITY UPLC I-Class Binary Solvent Manager delivers
solvent compositions for isocratic and binary gradient methods at flow rates of
0.01 to 2.0 mL per minute. Its features include in-line filters upstream of a
primary check valve, the Waters
®
Intelligent Intake Valve ( i
2
Valve ),
automated priming functions, and daily system-setup routines.
The design of the binary solvent manager (and, indeed, the system overall) is
optimized for sub-2-µm particle liquid chromatography. The upper pressure
limit is set at 124,106 kPa (1241 bar, 18,000 psi) per pump. A maximum flow
rate of 1 mL/min is permitted at system backpressures to 124,106 kPa
(1241 bar, 18,000 psi), which falls within the range of optimal linear velocity
for sub-2-µm particle columns of ID 1.0 mm to 3.0 mm.
See also:
The ACQUITY UPLC Binary Solvent Manager Operator's Overview
and Maintenance Information for more details .
Sample manager-FTN
The ACQUITY UPLC I-Class Sample Manager - FTN uses a
flow-through-needle mechanism that differs from a loop-based injector. The
mechanism aspirates a sample from plates and vials and holds it in the
sample needle in preparation for injecting the sample onto the column. The
needle serves as part of the injection flow path when the sample is pushed
onto the column.
Optional extension loops (installed between the sample needle and the
injection valve) can increase the injection volume beyond that of the sample
needle. The sample manager-flow through needle can also dilute samples
using the auto-dilution option
Using the flow-through-needle mechanism, the system operates similarly to
most traditional HPLC systems, which facilitates the transfer of HPLC
methods. The flow-through-needle mechanism also does not require you to
learn new injection modes. Moreover, it achieves injection accuracy and
decreases cycle time for small volume injections. Gradients pass through the
needle during injection, ensuring complete sample recovery.
Sample manager-FL
The sample manager - FL uses a loop-based mechanism to inject samples.
After a puncture needle pierces the well or vial covering, a sample needle