Waters ACQUITY UPLC I-Class, System Manual, Page: 20 / 68

1-8 ACQUITY UPLC I-Class System
emerges from within the puncture needle, to aspirate the sample. In the
partial loop needle overfill injection mode (default), the sample needle is then
removed from the vial and the sample syringe continues to pull the sample
aliquot through the sample needle and through the injection valve until any
pre-sample and the sample injection volume pass through the injection valve.
The valve actuates, switching the sample loop to the load position. The sample
is pushed back toward the needle and the sample volume is then pushed into
the sample loop. The sample loop is moved to the injection position and flow
from the pump pushes the sample onto the column.
See also:
ACQUITY UPLC Sample Manager - Fixed Loop Operator’s Overview
and Maintenance Information for information on other injection modes.
Column heater
Column temperature variations can shift peak retention times and alter peak
shapes, increasing the difficulty of achieving precise results. The ACQUITY
UPLC I-Class system’s column heater helps to ensure precise, reproducible
separations by controlling the column temperature.
The column heater (CH-A) heats the column compartment to any temperature
from 20 ºC to 90 ºC, that is at least 5 °C above the ambient temperature. A low
dispersion, active preheating device heats the incoming solvent before it
enters the column. The column compartment can accommodate columns of up
to 4.6 mm I.D. and up to 150 mm length.
30-cm Column heater (optional)
The optional 30-cm column heater (CH-30A) heats the column compartment to
any temperature, from 20 ºC to 90 ºC, that is at least 5 ºC above the ambient
temperature. The 30-cm column heater mounts on the side of the system
stack.
The 30-cm column heater can accommodate UPLC
®
columns of 4.6 mm, or
less, ID and 150 mm, or less, length and HPLC columns of 4.6 mm, or less, ID
and 300 mm, or less, length. For columns that exceed 4.6 mm ID, use the
ACQUITY UPLC 30-cm column heater/cooler with the appropriate
compatibility kit.
Note:
The ACQUITY UPLC 30-cm column heater/cooler is compatible with
I-Class systems. See ACQUITY UPLC Column Compartments Operator’s
Overview and Maintenance Information for details.