Waters 2996, Operator'S Manual

Page: 32 / 84

10 Installation
1
• Stainless steel tubing cutter or scribing file
• Pliers, plastic-covered, or with cloth
• Compression screw assemblies, three
Procedure
To make fluidic connections to the 2996 Detector:
1. Measure the lengths of tubing needed to connect:
• The column outlet to the 2996 Detector inlet
Note: Keep the length of this tubing as short as possible to prevent band
broadening.
• The 2996 Detector outlet to a waste collection bottle
Note: Ensure the length of this tubing is at least 1 to 2 feet (30 to 60 cm) to prevent
air bubbles from forming in the flow cell.
2. Cut the two lengths of tubing as follows:
a. Use a Waters 1/16-inch stainless steel tubing cutter or a file with a cutting edge to
scribe the circumference of the tubing at the desired break point.
b. Grasp the tubing on both sides of the scribed mark with cloth- or plastic-covered
pliers (to prevent marring the surface), then gently work the tubing back and
forth until it separates.
c. File the tubing ends smooth and straight to minimize dead volume and band
broadening.
3. Assemble a compression fitting (Figure 1-7) at both ends of the column outlet line
and at one end of the detector outlet line.