Waters 2996, Operator'S Manual

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58 Mobile Phase Absorbance
C
Waters PIC
®
Reagents
PIC A, 1 vial/L 0.67 0.29 0.13 0.05 0.03 0.02 0.02 0.02 0.02 <0.01
PIC B6, 1 vial/L 2.46 2.50 2.42 2.25 1.83 0.63 0.07 <0.01 — —
PIC B6, low UV,
1 vial/L 0.01 <0.01 — — — — — — — —
PIC D4, 1 vial/L 0.03 0.03 0.03 0.03 0.02 0.02 0.02 0.02 0.02 0.01
Detergents
BRI J 35, 1% 0.06 0.03 0.02 0.02 0.02 0.01 <0.01 — — —
CHAPS, 0.1% 2.40 2.32 1.48 0.80 0.40 0.08 0.04 0.02 0.02 0.01
SDS, 0.1% 0.02 0.01 <0.01 — — — — — — —
Triton
®
X-100, 0.1%
2.48 2.50 2.43 2.42 2.37 2.37 0.50 0.25 0.67 1.42
Tween™ 20,
0.1%
0.21 0.14 0.11 0.10 0.09 0.06 0.05 0.04 0.04 0.03
Table C-1 Mobile Phase Absorbance Measured Against Air or Water (Continued)
Absorbance at Specified Wavelength (nm)
200 205 210 215 220 230 240 250 260 280