Waters 2996, Operator'S Manual

Page: 42 / 84

20 Maintenance
3
3.1.1 Flushing the Flow Cell
Required Materials
• HPLC-grade water
• HPLC-grade methanol
If the flow cell requires cleaning, first try flushing it with solvent.
Procedure
To flush the flow cell:
1. Select a solvent compatible with the samples and mobile phases that you have been
using. If you have been using buffers, flush with HPLC-grade water for 10 minutes
at 1 mL/min, then switch to a low-surface-tension solvent such as methanol.
2. Set pump flow to 1 mL/min, then run the pump for 10 minutes.
3. Test the lamp energy by performing the Lamp diagnostic test. For details, refer to
the Millennium
32
Help.
If the lamp diagnostic fails and the lamp has not been used more than 2000 hours or
1 year (whichever comes first), disassemble the flow cell and clean the flow cell
components using the procedure described in Section 3.1.2, Removing the Flow
Cell.
3.1.2 Removing the Flow Cell
Note: You do not need to shut down the 2996 Detector to remove and replace the
flow cell.
Required Materials
• 5/16-inch open-end wrench
• Phillips screwdriver
• Powder-free gloves
STOP
Attention: Ensure that the solvent is miscible with the previous mobile
phase.