Waters 2996, Operator'S Manual

Page: 78 / 84

56 Mobile Phase Absorbance
C
Acids and Bases
Acetic acid, 1% 2.61 2.63 2.61 2.43 2.17 0.87 0.14 0.01 <0.01 —
Hydrochloric
acid, 0.1% 0.11 0.02 <0.01 — — — — — — —
Phosphoric acid,
0.1% <0.01 — — — — — — — — —
Trifluoroacetic
acid 1.20 0.78 0.54 0.34 0.22 0.06 <0.02 <0.01 — —
Diammonium
phosphate,
50 mM 1.85 0.67 0.15 0.02 <0.01 — — — — —
Triethylamine,
1% 2.33 2.42 2.50 2.45 2.37 1.96 0.50 0.12 0.04 <0.01
Buffers and Salts
Ammonium
acetate, 10 mM 1.88 0.94 0.53 0.29 0.15 0.02 <0.01 — — —
Ammonium
bicarbonate,
10 mM 0.41 0.10 0.01 <0.01 — — — — — —
EDTA, disodium,
1 mM 0.11 0.07 0.06 0.04 0.03 0.03 0.02 0.02 0.02 0.02
HEPES, 10 mM,
pH 7.6 2.45 2.50 2.37 2.08 1.50 0.29 0.03 <0.01 — —
MES, 10 mM,
pH 6.0 2.42 2.38 1.89 0.90 0.45 0.06 <0.01 — — —
Table C-1 Mobile Phase Absorbance Measured Against Air or Water (Continued)
Absorbance at Specified Wavelength (nm)
200 205 210 215 220 230 240 250 260 280