Varian ProStar 400 Operation Manual Download Page 1

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Varian, Inc.
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/usa

©Varian, Inc. 2000 - 2004

Printed in U.S.A.

03-914786-00:Rev. 3

ProStar 400

AutoSampler

Operation Manual

Summary of Contents for ProStar 400

Page 1: ...Varian Inc 2700 Mitchell Drive Walnut Creek CA 94598 1675 usa Varian Inc 2000 2004 Printed in U S A 03 914786 00 Rev 3 ProStar 400 AutoSampler Operation Manual...

Page 2: ......

Page 3: ...zed standards It means we live up to a standard of quality that you can count on today and into the future Our Quality System has received ISO 9001 certification number FM21797 The quality systems tha...

Page 4: ...e purchase price of the instrument or accessory Repair or replacement under warranty does not extend the original warranty period Repair or replacement under warranty claims shall be made in Varian s...

Page 5: ...oderate injury and or equipment damage and how to avoid these situations Alerts you to potentially hazardous situations that could result in serious injury and how to avoid these situations Warning Sy...

Page 6: ...has been used for the analysis of hazardous biological radioactive or toxic samples prior to any instrument service being performed or when an instrument is being returned to the Service Center for re...

Page 7: ...graphy The operator should be familiar with the physico chemical properties of the components of the mobile phase Keep solvents from direct contact with the polyurethane supply tubing as certain solve...

Page 8: ...nical support and training can be provided by qualified personnel on both a contractual or as needed basis Varian Inc Analytical Instruments Sales Offices For Sales or Service assistance and to order...

Page 9: ...uf die Vermeidung dieser Situationen hin Warnungssymbol Warnungsbeschreibung WARNUNG ELEKTRISCHER SCHLAG Gef hrliche Spannungen bestehen innerhalb des Instruments Trennen Sie das Ger t vom Netz bevor...

Page 10: ...nt f r Analysen gef hrlicher biologischer radioaktiver oder toxischer Proben benutzt worden ist Elektrische Gefahren L sen Sie das Instrument von allen Stromquellen bevor Sie Schutzpaneele entfernen d...

Page 11: ...n durchf hren ffnen Sie niemals eine unter Druck stehende L sungsmittelleitung oder ein Ventil Halten Sie zuerst die Pumpe an und lassen Sie den Druck auf Null abfallen Benutzen Sie splittersichere Re...

Page 12: ...zung und Training bieten wir Ihnen durch qualifizierte Chemiker sowohl auf einer Kontraktbasis als auch nach Ihren Erfordernissen an Varian Analytical Instruments Verkaufsb ros F r Verkaufs oder Servi...

Page 13: ...mment rem dier cette situation Symboles d avertissement Description ATTENTION RISQUE D ELECTROCUTION Exposition des tensions dangereuses D brancher le mat riel du secteur avant de d visser les panneau...

Page 14: ...giques dangereux radioactifs ou toxiques avant que n en soit effectu la maintenance Risques de chocs lectriques D connecter le mat riel de toute source d alimentation avant d en d monter les panneaux...

Page 15: ...p rations de maintenance de routine Ne jamais d connecter un conduit de solvant ou une vanne sous pression Arr ter pr alablement la pompe et laisser la pression descendre z ro Utiliser des r servoirs...

Page 16: ...el sp cifique support technique et service de formation assur s par des chimistes qualifi s sur base contractuelle ou en fonction des besoins sp cifiques Points de vente des instruments analytiques Va...

Page 17: ...ZIONE Descrizione del Pericolo ATTENZIONE Pericolo di folgorazioni Nello strumento sono presenti tensioni pericolose Scollegare il cavo di alimentazione prima di togliere il pannello fissato con le vi...

Page 18: ...zzato per l analisi di campioni biologicamente pericolosi radioattivi o tossici Pericoli Elettrici Prima di togliere i pannelli di protezione scollegare lo strumento da tutte le alimentazioni elettric...

Page 19: ...routinaria del sistema Non smontare mai una linea del solvente od una valvola quando il sistema sotto pressione Fermare prima la pompa ed aspettare che la pressione scenda a zero Usare dei contenitor...

Page 20: ...a piu il costo delle parti A richiesta si possono avere corsi per operatori sia sotto forma di contratto che a tariffe da concordare Uffici Vendite della Divisione Strumenti Analitici della Varian Per...

Page 21: ...grosas que pueden causar serios da os y c mo evitar esas situaciones S mbolo Descripci n ATENCI N PELIGRO DE DESCARGA EL CTRICA El instrumento utiliza voltages peligrosos Desconecte el interruptor gen...

Page 22: ...stras peligrosas de origen biol gico radioactivo t xico antes de comenzar a realizar cualquier an lisis Peligros El ctricos Desconecte el instrumento de todos las conexiones el ctricas a la red antes...

Page 23: ...e rutina Nunca abra una l nea una v lvula bajo presi n Apague la bomba antes y deje que la presi n baje a cero Utilice dep sitos irrompibles que sean capaces de operar a 50 60 psi Mantenga cerrada la...

Page 24: ...a y materiales El mantenimiento y el entrenamiento se realiza por ingenieros cualificados bajo Contrato petici n Oficinas de Instrumentaci n Anal tica Varian Para cualquier consulta sobre Instrumentac...

Page 25: ...mple Consumption 8 Rest volume 8 Pre flush volume 8 Injection volume 9 Sample consumption formula dispenser controlled 9 Sample consumption for headspace pressure controlled loopfill 9 Filling and Sea...

Page 26: ...ngs 21 RUN Parameters 22 Cooling Control optional 23 RUN 23 HOLD 24 Priority Sample 25 Remote Control 25 PC Workstation Interface 26 Varian 4400 Integrator Interface 27 Special Display Messages 27 Aft...

Page 27: ...of large series of samples in an environment where zero sample loss and injection flexibility are of little significance but where reliability stability and reproducibility are mandatory The ProStar...

Page 28: ...2 03 914786 00 3...

Page 29: ...stomer Service 1 800 FOR HPLC or your local Varian office For contents of shipping container see the Standard Accessory Kit list in the shipping box Unpack the ProStar 400 AutoSampler carefully Connec...

Page 30: ...our power source plug in the power cable The correct fuses should be installed WARNING FIRE HAZARD For continued protection replace only with same type and rating of fuse If a new voltage was selected...

Page 31: ...active low 10 Stop I O TTL pulled up output is low when the ProStar 400 AutoSampler ends processing READY pull low input will stop processing ProStar 400 goes to READY 11 Spare open collector 12 Next...

Page 32: ...ed by a PC using the RS485 interface Fluid Connections The instrument has been tested with isopropanol Make sure that the mobile phase of your HPLC system is miscible with isopropanol If not start up...

Page 33: ...han 100 L and twice the loop volume for loops larger than 100 L Loops ranging from 5 L to 1 mL are available from Varian Digital Dispenser System DDS The DDS pump tubing cassette is factory installed...

Page 34: ...vial out of reach of the sample needle This volume is called the rest volume For standard 1 5 mL vials the rest volume is approximately 200 L For a conical vial the rest volume is only a few L Refer t...

Page 35: ...olume 3N x loopvolume N 1 x 4 L Example 3 flushed loop injections out of a standard sample vial using a 20 L loop and 30 L pre flush volume minimum sample volume needed is 200 L 30 L 3 x 3 x 20 L 2 x...

Page 36: ...ill with headspace pressure NOTE Do not reuse a vial without replacing its septum Loading the Sample Tray To allow remote loading of the sample tray the sample tray consists of four removable segments...

Page 37: ...nt 1 5 mL each usually mobile phase on positions 93 96 You must use all four vials as they will be used alternately Large Volume Samples Description The large volume sample tray is an option of the Pr...

Page 38: ...he 500 L loop to port 1 and 4 Check the needle penetration depth and if necessary adjust it See page 29 Needle Pair Replacement Connect the drain to port 3 if the loop will be filled on a time basis b...

Page 39: ...mple tray consisting of 4 removable segments 2 Keyboard 3 Display 4 Removable cover 5 Needle arm with sample needle 6 Injection valve with sample loop 7 Dispenser cassette screw 8 Dispenser 9 Outlet t...

Page 40: ...ure applied to the sample through the outer air needle ensures that no air or vapor bubbles are formed during sample withdrawal 3 Dispenser withdraws preflush volume from sample vial to fill the sampl...

Page 41: ...d on the sample through the outer air needle will force the sample via the inner sample needle through the sample loop The duration of the headspace pressure is programmable appropriate duration has t...

Page 42: ...Digital Dispenser System DDS Operating Principle The DDS is a miniature finger pump The operating principle is shown below Fingers 1 and 3 act as inlet and outlet pinch valve respectively finger 2 pro...

Page 43: ...g is always turned off at power up Principle Different Programming Modes To simplify regular operation common injection parameters like analysis time and first last vial are programmed in the RUN PARA...

Page 44: ...de and RUN mode which are left via PROG END and STOP respectively Key Functions STEP key used to step through the parameter list The STEP key is also used to rotate the sample tray in READY status onl...

Page 45: ...ion code Selected mode F 9 Selects SYSTEM SETTINGS mode F 7 Selects COOLING CONTROL mode only if cooling option is installed F 2 Selects PRIORITY mode only accessible during sample run F 3 Selects REM...

Page 46: ...N 0 00 99 59 9 TIME AUX 2 OFF 0 00 99 59 Use to step 1 LOOPVOLUME 0 999 L 2 SAMPLE VOLUME STD LSV 3 FLUSH VOL TIME 4 NEEDLE WASH YES NO 5 AUXILIARIES YES NO TRAY COOLING ON OFF PRESS TO ADVANCE SAMPLE...

Page 47: ...led loopfill this will set the loop overfill volume to 3X loopvolume if loop 100 L and 2X loopvolume for loops 100 L SAMPLE VOLUME STD LSV Enter 0 to select STANDARD sample tray Enter 1 if the optiona...

Page 48: ...he following parameters can be programmed use STEP key to go to next parameter FIRST VIAL Enter position number of the first vial of the sample series in the sample tray 1 96 without needle wash 1 92...

Page 49: ...t COOLING CONTROL mode Press PROG END to switch ON or OFF the cooling control The sample tray cooling has a fixed temperature of 4 C TRAY COOLING ON OFF Enter 0 to switch OFF the sample tray cooling E...

Page 50: ...AMETERS during RUN simply press PROG END to enter the RUN PARAMETER mode Press PROG END to exit RUN PARAMETER mode and to continue the RUN program NOTE If you do not return to RUN mode RUN will stop a...

Page 51: ...Star or Star LC modules the ProStar 400 is interfaced using Cable Kit Part Number 03 909126 90 Remote control mode is not necessary to use this cable However using remote control mode the ProStar 400...

Page 52: ...will no longer have any effect Exit REMOTE CONTROL mode F 0 to return to READY status PC Workstation Interface Up to four ProStar 400 AutoSamplers can be simultaneously controlled from the Varian Star...

Page 53: ...with this cable describe how to interface to the 4400 such that the AutoSampler vial number is printed on each 4400 report Full synchronization cable operation of the ProStar 400 with the 4400 Integra...

Page 54: ...nue NEEDLE WASH was switched ON SYSTEM SETTINGS with FIRST or LAST vial programmed on WASH positions FIRST VIAL and LAST VIAL are reset to 0 reprogram RUN PARAMETER mode Press any key to continue ERRO...

Page 55: ...port 2 10 Sample tubing valve port 3 dispenser 11 Sample loop 12 Sample drain for loopfilling by sample headspace pressure Replaces sample tubing 10 13 Vial stripper 14 The silicon tubing connector wi...

Page 56: ...0 L in case of flushed loop injections Press START and disconnect the power supply as soon as the needle is in the vial Loosen adjustment screw one turn counterclockwise Using the needle connection n...

Page 57: ...own manually Remove sample needle by pulling it out of its fitting with the PTFE tubing that usually sticks to the needle Put in new needle watch the air seal around the needle When needle connection...

Page 58: ...l sample needle Dispenser Pump Tubing Loosen fitting that connects PTFE inlet tubing of dispenser to injection valve Remove pump tubing cassette including PTFE connection tubing by loosening the screw...

Page 59: ...ssure Injections per vial max 9 Analysis time max 99 min 59 sec Needle wash Selectable 50 L fixed volume between vials Headspace pressure Built in compressor 0 5 atm or 7 psi Switching time injection...

Page 60: ...xt vial input TTL Freeze runtime TTL Stop I O TTL OPTIONS Sample tray cooling Built in Peltier cooling Temperature 4 C fixed Accuracy 2 C Operation range Ambient 25 C Ring temperature at relative humi...

Page 61: ...0190000804 Serum Needle for Standard Vials Package of 3 0190000805 Air Serum Needle for Standard Vials 0190000836 Serum Needle for Large Volume Option Package of 3 0190000837 Air Serum Needle for Larg...

Page 62: ...3 Valco fingertight PEEK Nut 0392607984 Valco fingertight PEEK Ferrule VIALS SEALS AND CAPS R0000542CV Standard clear crimptop 2 CV vial pck 100 R005411AC6 Crimpcap for standard 2 CV and 1 1 CTVG vial...

Page 63: ...ls Screw Caps and Septa 1 gross 12 gross 6600010400 Amber Glass 2 mL Vials 1 gross 0099743106 0099743105 Tapered Glass 1 1 mL Vials requires Teflon Support Sleeve 500 50 6600012101 Precision 100 L Via...

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