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Troubleshooting Guide

7. Calibrate

the

grating

a.  Move the grating to 10 steps below the peak.
b.  Scan to 10 steps above the peak.
c.  Find the actual energy peak in the scan.
d.  On a VIS instrument, repeat steps 7.a – 7.c for 4 wavelengths

i. 494.0nm

peak

ii. 585.2nm

valley

iii. 775.0nm

peak

iv. 807.1nm

valley

e. On the UV instrument, repeat steps 7.a – 7.c for 4 wavelengths

i. 824.1nm

peak

ii. 882.7nm

peak

iii. 895.8nm

peak

iv. 905.2nm

peak

v. 917.0nm

peak

f. Set the final calibration by the average of the errors from the calibration peaks.

8.

On the VIS instrument, move to 681nm and determine the ADC integration time.

9.

Move to the last wavelength used on the walkup (ATC) screen, and zero the system.

Spectronic GENESYS 10 UVscanning, Spectronic GENESYS 10 > Revision 1.103, Spectronic
BioMate 3 > Revision 1.001 and Spectronic GENESYS 10 Bio > Revision 1.000 Initialization

1. Power

on.

2. Calibrate

turret

a. Rotate the turret while reading the turret sensor.
b. After we have detected 3 small openings without finding the big opening, reverse the

turret rotation direction.

c. After we have detected 6 more small openings without finding the big opening, return a

turret calibration failure error.

d. When we find the big opening (indicates position B), center the turret in the opening.

3.

Initialize the grating
a.  Moving to the grating calibration switch.
b.  On the VIS instrument, move to grating step 8695 (ideally 1095nm).
c.  On the Spectronic GENESYS 10 UVscanning, move to grating step 15948 (ideally

716nm).

d. On other UV instruments, move to grating step 6475 (ideally 850nm).

4.

Initialize the filter wheel
a.  Rotate the filter wheel while recording energy levels.
b.  Look for an area that passes light.
c.  Center the filter wheel in the position found.

5.

Find zero order
a. Spectronic GENESYS 10 UVscanning.

i. Scan between grating steps 1472 and 2432 for the start of zero order saturation.

ii. Scan towards grating step 3968 for the end of zero order saturation.

b. Spectronic GENESYS 10, Spectronic BioMate 3 and Spectronic GENESYS 10 Bio.

i. Scan between grating steps 555 and 2035 for the start of zero order saturation.

ii. Scan towards grating step 2405 for the end of zero order saturation.

c. Set the initial calibration to the center of the zero order area.

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Summary of Contents for Spectronic BioMate 3

Page 1: ......

Page 2: ...h regard to this material including but not limited to the implied warranties of merchantability and fitness for a particular purpose Copyright 2002 2004 by Thermo Electron Corporation Madison WI 53711 All world rights reserved No part of this publication may be stored in a retrieval system transmitted or reproduced in any way including but not limited to photocopy photograph magnetic or other rec...

Page 3: ...ructions carefully This symbol alerts you to potential electrical hazards Be sure that only qualified persons perform the related procedures This symbol alerts you to hot surfaces Be sure to read and follow the associated instructions carefully This symbol alerts you to potential UV radiation exposure which can cause eye damage Wear UV opaque eye protection iii ...

Page 4: ...CLUDING BUT NOT LIMITED TO WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE OR MERCHANTABILITY OR OTHERWISE and states Thermo Electron Corporation s entire and exclusive liability and the Customer s exclusive remedy for any claim in connection with the sale or furnishing of services goods or parts their design suitability for use installation or operations Thermo Electron Corporation will in no even...

Page 5: ...o 190 1100 5 List A and B 100nm max per scan Spectronic GENESYS 10 UVscanning 190 1100 5 List A 190nm 1100nm Spectronic GENESYS 6 190 1100 2 List A 190nm 1100nm Refer to the operator s manual for additional information Software List A Software List B Basic A T C Nucleic Acid Tests Advanced A T C Protein Tests Standard Curve Cell Growth Absorbance Ratio Oligo Calculator Absorbance Difference Kineti...

Page 6: ...This page intentionally left blank vi ...

Page 7: ...mator cover 5 8 Limit Switch 335101 654S 5 9 Filter wheel 335901 120S Vis Only 335902 120S UV Vis Only 5 10 Sample Detector 335901 6034 UV Vis Only 335901 6044 Vis Only 5 10 Reference Detector 335901 6034 UV Only 5 13 Turret motor 335901 660S UV and Vis 5 15 Light Source 335923 000 Vis Only 335902 146S UV Vis Only and 335908 Spectronic GENESYS 6 Only 5 17 Mirrors 5 18 Grating assembly 335901 125 5...

Page 8: ...tions 9 4 Connect 9 4 Disconnect 9 4 Reset Spectronic GENESYS 10 9 5 Query Instrument 9 5 Update Settings 9 5 Calibrate 9 5 Filter and turret 9 6 Alignment Tool 9 8 Seek Limit Switch 9 8 Seek zero order 9 9 Flash Xenon 9 9 Detector board Diagnostic 9 9 Lamp 9 13 CHAPTER 10 RS232 COMMANDS 10 1 Data commands 10 1 Instrument control commands 10 2 Miscellaneous commands 10 2 Immediate Commands Special...

Page 9: ...directional RS232C and parallel output in PCL format Cell Holder 1 position or automatic 6 position cell holder Keypad Membrane keypad Software Absorbance Transmittance Concentration Standard Curve Absorbance Ratio Absorbance Difference Kinetics Survey Scan 3 Point Net Multiwavelength Performance Validation As Sepctronic GENESYS 10 UV but with full range scanning Scanning speed 200 1000nm min Samp...

Page 10: ...d Interface Bi directional RS232C parallel output in HP PCL format Cell Holder Automatic 6 cell positioner Keypad Membrane keypad Scanning Speed 10 2 000nm min Slew rate 11 000nm min Scanning Sampling Interval 0 1 0 2 0 5 1 0 2 0 5 0nm Disc Drive 3 5 floppy 1 44 Mb Test Storage Up to 80 tests in non volatile memory up to 256 on a floppy disk Languages user selectable Software and Printout English ...

Page 11: ...al RS232C Cell Holder 1 position or automatic 6 position cell holder Keypad Membrane keypad Software DNA ratio concentration with or without scanning Direct protein at 280nm Protein standard curves Cell growth Oligo calculator absorptivity molecular weight factor and theoretical TM Absorbance Transmittance Concentration Absorbance Ratio Absorbance Difference Standard Curve Kinetics Survey Scan Per...

Page 12: ...m up period Ambient temperature 5 to 35 C 41 F to 95 F Relative humidity 20 80 RH Storage environment 20 C to 70 C 4 F to 158 F Relative humidity not to exceed 0 040 pounds moisture per pound of dry air Allow instrument to adjust to room temperature for 24 hours after taking it out of storage Temperature should be maintained at 4 F Relative humidity should be maintained to 5 Altitude From below se...

Page 13: ...Optical Diagram Figure 1 1 Optical Diagram Shown are both UV Vis and Vis Optics 1 5 ...

Page 14: ...Specifications This page intentionally left blank 1 6 ...

Page 15: ...tion Performance Checklist Inspection Performance Checklist Master Copy Make a copy of the Field Inspection Performance Checklist on the next page fill it out and leave it with your customer after each service call 2 1 ...

Page 16: ...rget Pass Fail RS232 Serial Port Pass Fail Optional Printer Pass Fail Optional Analog output Pass Fail Not installed Not installed Voltage Checks Expected Measured Expected Measured 5 0 25v 12v Analog 0 6v 12v 1 2v 12v Analog 0 6v 12v 1 2v Lamp Voltage Vis only 5 7v to 6 1v Wavelength Accuracy SPECTRONIC Standard Expected Measured _______ 3nm Internal Wavelength Test UV only Internal Wavelength Te...

Page 17: ...ve detected 3 small openings without finding the big opening reverse the turret rotation direction c After we have detected 6 more small openings without finding the big opening return a turret calibration failure error d When we find the big opening indicates position B center the turret in the opening 3 Initialize the grating by a Moving to the grating calibration switch b On the VIS instrument ...

Page 18: ... reverse the turret rotation direction c After we have detected 6 more small openings without finding the big opening return a turret calibration failure error d When we find the big opening indicates position B center the turret in the opening 3 Initialize the grating a Moving to the grating calibration switch b On the VIS instrument move to grating step 8695 ideally 1095nm c On the Spectronic GE...

Page 19: ...1nm valley f On the UV instrument repeat steps 7 a 7 c for 4 wavelengths v 824 1nm peak vi 882 7nm peak vii 895 8nm peak viii 905 2nm peak ix 917 0nm peak g Set the final calibration by the average of the errors from the calibration peaks 9 On the VIS instrument move to 681nm and determine the ADC integration time 10 Move to the last wavelength used on the walkup ATC screen and zero the system Spe...

Page 20: ...d of the large dark area to locate the filter wheel d Center the filter wheel on the next position the open position 7 Initialize the calibration gains a Select the appropriate filter for 224nm b Scan from 224nm to 235nm c Zero the instrument at the grating step with the highest energy level from the above scan 8 Calibrate the grating a Scan from 242nm to 253nm looking for the peak at 247 59nm b S...

Page 21: ... Vis Filter Positions Position Beginning Wavelength Ending Wavelength 1 2 3 325 429 4 430 619 5 620 1100 6 7 8 Table 4 Vis Filter Positions Position Beginning Wavelength Ending Wavelength 1 2 3 325 359 4 360 419 5 420 519 6 520 649 7 650 1100 8 Didymium 3 5 ...

Page 22: ...Troubleshooting Guide This page intentionally left blank 3 6 ...

Page 23: ...her broken or disconnected Ensure cable securely connected Replace cable Cable from display board to connect J9 on Main board is either disconnected or broken Ensure cable securely connected Replace cable Display cables are switched Install Display cables correctly Display malfunctioning Replace display Display does not light up upon power up Main board problem Replace Main board Display lights up...

Page 24: ... cable from lamp to J12 on Main board is either broken or disconnected Ensure cable is securely connected Replace cable Optical path not aligned Align the optics Detector unplugged or cable installed incorrectly Ensure that Detector is securely connected Filter wheel turns but displays Cannot calibrate the filter wheel message Instrument not recognizing changes in energy Check cable connections Ch...

Page 25: ...sed and ENTER keys simultaneously at power up Run Service software to recalibrate instrument and reset display Battery not installed or is dead Install or replace battery and run Service software to recalibrate instrument and reset display Display is dark background with light text Main board problem Replace Main board and run Service software to recalibrate instrument and reset display Battery no...

Page 26: ...alternate keypad works OK then replace the defective keypad If the alternate keypad demonstrates the same failure replace the Main board The following keyboard key groups do not respond when pressed other keys function OK Turret 1 turret 2 turret 3 CLEAR F4 and or Turret B turret 4 turret 5 ENTER F3 and or PRINT 3 6 9 F2 and or TEST 0 2 5 8 F1 and or UTILITY 1 4 7 ESC Main board problem Replace th...

Page 27: ...er dirty or broken Clean or replace Beam splitter Detector board not plugged in or a cable is broken Ensure cable is securely connected Replace Detector board Gain jumper incorrectly set on reference Detector board Install jumper correctly Detector board configuration jumpers are set incorrectly Vis 2 3 jumpered UV 1 2 jumpered and 3 4 jumpered Install jumpers correctly Detector problem Replace De...

Page 28: ...in board malfunctioning Replace Analog board if this does not resolve problem replace the Main board Incorrect signal to YT recorder Analog board or Main board malfunctioning Replace Analog board if this does not resolve problem replace the Main board Analog board not enabled in firmware Service Diagnostic software No signal to YT recorder does not pass Analog test Analog board or Main board malfu...

Page 29: ... on The dust cover supplied with the instrument is resistant to most aqueous solutions Do not use or store the instrument in a corrosive environment Gently wipe the outside of the instrument with a soft cloth to remove any dust or spills Water isopropyl alcohol and other common laboratory cleaning agents may be used if necessary Always clean up spills to prevent or minimize damage to the instrumen...

Page 30: ...nce readings Cells should be protected during cleaning and never thrown into a bath with glassware where they might get scratched or placed in a wire rack that might scratch them The outside of the cells may be wiped with a soft lint free tissue and should be kept free of fingerprints Micro Flowcells Micro flowcells can be kept clean by Flushing well with a solvent after use Aspirating dilute acid...

Page 31: ...g the instrument before removing the cover of the lamp compartment WARNING To avoid personal injury or damage to the instrument follow these instructions carefully 1 Turn off and unplug the instrument 2 Make sure that the lamp has had time at least 10 minutes to cool off Figure 4 1 Replacing the Tungsten Lamp Key Captive screw on lamp door Thumbscrew Lamp assembly Tab on cable connector Aligning t...

Page 32: ...lamp with your fingers 8 Unpack the new lamp assembly 9 Use a soft lint free cloth to wipe off any fingerprints on the lamp 10 Connect the cable to the connector 11 Position the new assembly as shown in 5 of Figure 4 1 making sure to align the pins correctly 12 Tighten the thumbscrew 2 Figure 4 1 13 Close the lamp door 14 Plug the instrument into an appropriate wall outlet and turn on the power 15...

Page 33: ...the same type and rating fuse WARNING If the fuse fails repeatedly it may indicate a serious problem with the instrument Contact technical support as soon as possible Figure 4 2a Back Panel of the Spectronic GENESYS 10 Spectrophotometer Key A C power connector RS232C port Analog BNC connector optional Figure 4 2b Back Panel of the Spectronic GENESYS 10 UVscanning and Spectronic GENESYS 6 Spectroph...

Page 34: ...ver into the notch Figure 4 3 on the fuse cover and pry off the cover Figure 4 3 Removing the cover of the Fuse Component 4 Use a flat blade screwdriver to remove the fuse holder Figure 4 4 Figure 4 4 Removing the Fuse Holder 5 Unsnap both fuses Figure 4 4 to remove them Figure 4 5 Removing and Replacing the Fuses 6 Insert the new fuses pushing them in so they snap into place 7 Replace the fuse co...

Page 35: ...the Spectronic GENESYS Series spectrophotometers 335980 Internal Printer assembly For the Spectronic BioMate 3 and Spectronic GENESYS 10 Bio spectrophotometers 335923 Tungsten Lamp assembly For all Spectronic GENESYS 10 Vis spectrophotometers all catalog numbers beginning with 335900 and 335901 333152 Sensitized Paper 100 sheets for SPECTRONIC Standards 335965 Replacement Dust cover For all Spectr...

Page 36: ...Routine Maintenance This page intentionally left blank 4 8 ...

Page 37: ...H SANDPAPER OR FILES WHEN CLEANING THE INSTRUMENT WHEN SERVICING IS COMPLETE THOROUGHLY INSPECT THE INSTRUMENT FOR LOOSE BOARDS OR CABLES COLD SOLDER JOINTS LOOSE HARDWARE WIRE BITS AND LOOSE OR MISSING SCREWS External Covers WARNING When in operation certain internal components such as the lamp and Power Supply become hot and may still be hot when the unit is opened for servicing Avoid touching t...

Page 38: ... 1 Turn the unit over so it rests on its side 2 Position the base cover on the bottom of the instrument Make sure the pins at the back of the instrument fit into the holes in the cover 3 Replace and tighten the four screws Bottom cover Power Supply Removal 1 Remove top cover see top cover removal instructions 2 Disconnect cable from Power Supply 3 Turn the unit over so it rests on its side 4 Remov...

Page 39: ...ply Removal Replacement Replacement 1 Turn the unit over so it rests on its side 2 Position the base cover on the bottom of the instrument 3 Replace and tighten the three screws 4 Connect power cable 5 Replace top cover see top cover replacement instructions 5 3 ...

Page 40: ...ift up on the front of the instrument and press the two tabs outward to release them from their clips refer to Figure 5 4 While holding the tabs outward press the tabs up through the bottom of the instrument Figure 5 4 Spectronic GENESYS 10 Spectrophotometer Bottom View Showing Tabs CAUTION THE DISPLAY AND KEYPAD CABLES ARE CONNECTED BETWEEN THE COVER AND THE MAIN CIRCUIT BOARD IN THE INSTRUMENT B...

Page 41: ...he Main Circuit board 6 Disconnect the connector for the keypad from connector J13 of the Main Circuit board 7 Disconnect the printer cable from connector J11 of the Main Circuit board Figure 5 5 Connector Locations 8 Set the cover aside Replacement 1 If they were disconnected connect the printer cable to J11 the keypad cable to J13 and the display cables to J9 on the Main Circuit board All connec...

Page 42: ...rotruding through the tab slot in the bottom of the instrument 5 Push the cover to the left until you feel the front left corner tab protrude through the tab slot in the bottom of the instrument 6 Press the cover straight down until the tabs lock into place 7 Access the rear of the instrument and tighten the two mounting screws refer to Figure 5 3 Internal Assemblies NOTE Prior to working on any o...

Page 43: ...acement NOTE The Beam Splitter and turret cover have one screw in common with the Sample Detector cover Make sure the Sample Detector cover is in place and the common screw is not in place prior to locating the Beam Splitter and turret cover 1 Make sure the Sample Detector cover has been replaced 2 Position the cover over the beam splitter and turret area 3 Replace and tighten the three screws whi...

Page 44: ...r in place Figure 5 8 Monochromator cover 2 Carefully lift the cover off the assembly and set aside Replacement 1 Position the cover over the monochromator and mirrors 2 Replace and tighten the two screws which hold the cover Figure 5 9 Filter wheel Limit Switch and Sample Detector Components 5 8 ...

Page 45: ... wire assembly to the terminals of the limit switch 2 Position the limit switch with its actuator button positioned as shown in Figure 5 4 Make sure the grating arm makes proper contact with the limit switch at the end of the arm s rotation 3 Insert and tighten the two screws which secure the limit switch 4 Route the limit switch wiring through the channel in the base so that the Monochromator cov...

Page 46: ...crew with washers through the center hole of the filter wheel making sure the orientation of the wheel is proper so it will mesh with the drive gear 2 Locate the filter wheel in position and tighten the screw 3 When all other work is complete replace the Monochromator cover refer to Monochromator Sample Detector 335901 6034 UV Vis Only 335901 6044 Vis Only Removal 1 Remove the Beam Splitter and tu...

Page 47: ...INGERS 5 Remove the Sample Detector Circuit board 335901 6034 UV or 335901 6044 Vis Figure 5 11 Sample Detector 6 Remove the two screws securing the Sample Detector Circuit board to the base 7 Carefully pull the Sample Detector Circuit board away from its locating dowel pins and remove it from its mounting position 8 Remove the ribbon cable from its connector on the Sample Detector Circuit board 9...

Page 48: ...ce the Sample Detector Circuit board 335901 6034 UV or 335901 6044 Vis NOTE The detectors are specific to the instrument type UV vs Vis so you cannot interchange the two UV Detector boards and the Vis Detector boards by simply changing the jumper Ensure that you have the correct board and the correct jumper configuration W1 Jumper Settings Vis Sample Detector pins 2 and 3 are jumpered UV Sample De...

Page 49: ...sition the cover over the Sample Detector Circuit board and lens 10 Insert and tighten the lower screw and the one upper screw which is not common to the Beam Splitter and turret cover 11 After all replacements are complete for mirrors light source grating and lenses follow the optical alignment procedures in Optical Alignment 12 When all other work is complete replace the Beam Splitter and turret...

Page 50: ...ve the ribbon cable from its connector on the Reference Detector Circuit board 10 Remove the Lens if cleaning or replacement is required CAUTION NEVER TOUCH THE LENS WITH YOUR FINGERS ALWAYS USE AN UNCOATED LENS TISSUE OR SOFT COTTON CLOTH OR CLEAN LAB GLOVES TO AVOID OILS GETTING ONTO THE LENS NEVER USE A LENS PAPER WITH SILICONE ADDITIVES NOTE The lens does not have an orientation It fits into a...

Page 51: ...ion making sure the holes on the Circuit board fit over the two dowel pins 7 Replace and tighten the two mounting screws 8 Replace the Reference Detector cover 9 Position the cover over the Reference Detector Circuit board and lens 10 Insert and tighten the three screws to secure the cover 11 After all replacements are complete for mirrors light source grating and lenses follow the optical alignme...

Page 52: ...otor to the base 8 Pull the motor straight up through the hole in the base 9 Fit the wire connector up through the hole in the base being careful not to damage any of the other wires passing through the hole if not done in the previous step and set the motor aside 10 Remove the Main Circuit board refer to Main board Connections 11 Remove the turret shaft by looseing the set screw that holds the ge...

Page 53: ... which secure the motor to the base 7 Pass the connector end of the motor wires through the hole in the base making sure the other wires passing through the hole are not damaged 8 If all other work is complete replace the Main Circuit board refer to Main board Connections 9 Attach the connector on the end of the motor wires to the Main Circuit board 10 Replace the turret Opto Switch 11 Insert and ...

Page 54: ...n the bracket 2 Set the light source bracket in position on the dowel pins 3 Insert and tighten the screws holding the light source bracket to the base 4 Turn the unit over and attach the light source wire connector to the Main Circuit board Mirrors CAUTION DO NOT TOUCH THE MIRROR SURFACE NOTE The four mirrors in the unit are different Replace the A 335901 100S B1 335901 105S B2 335901 106S or C 3...

Page 55: ...e grating and lenses follow the optical alignment procedures in Optical Alignment 3 When all other work is complete replace the Monochromator cover refer to Monochromator 4 When all other work is complete replace the Beam Splitter and turret cover refer to Beam splitter and turret cover Grating assembly 335901 125 CAUTION DO NOT TOUCH THE GRATING SURFACE WITH ANYTHING Removal 1 Remove the Beam Spl...

Page 56: ... shaft making sure the collar stays in place around the neck of the grating assembly 3 Slip the spacing tool from the alignment kit between the grating and the grating motor to provide 0 5mm space This space prevents any friction between the grating mount and the top of the motor 4 Tighten the screw on the clamp collar 5 After all replacements are complete for mirrors light source grating and lens...

Page 57: ...tor to the base 4 Carefully remove the grating motor so the shaft comes out of the base and set the motor aside Replacement 1 From the bottom of the unit insert the shaft of the grating motor through the hole in the base and turn the grating motor so it s threaded mounting holes are aligned with the mounting holes in the base 2 Insert and tighten the two screws used to secure the grating motor to ...

Page 58: ...acket in place it only needs to be loose enough to allow room to remove the grating motor Block assembly 4 Remove the two 8 32 screws that secure the grating motor block assembly to the base 5 Carefully remove the grating motor Block assembly so the shaft comes out of the base and set the motor aside Replacement 1 From the bottom of the unit insert the shaft of the grating motor Block assembly thr...

Page 59: ...t the back of the drive note orientation Power Supply 335901 653 WARNING When in operation certain internal components such as the lamp and Power Supply become hot and may still be hot when the unit is opened for servicing Avoid touching these areas Failure to observe this warning may result in burns Removal 1 Disconnect the wire connector for the Power Supply wires from the top of the Main Circui...

Page 60: ...TIC SENSITIVE ASSEMBLIES OR DEVICES WHEN HANDLING PRINTED CIRCUIT BOARDS OR OTHER ELECTRONIC ASSEMBLIES HOLD THE BOARD BY ITS EDGES TO AVOID TOUCHING CIRCUIT TRACES OR DEVICES WITH BARE HANDS OR FINGERS Removal Figure 5 21 Main Circuit board Connector Locations 1 Disconnect all wire connectors on the top and end of the Main Circuit board accessible at the rear of the instrument through holes in th...

Page 61: ...4 Turn the unit over and attach the remainder of the wire connectors to the top and end of the Main Circuit board 5 If the firmware revision of the new board is 1 030 then replace the ROM and flash it to the current version If the firmware revision of the new board is 1 030 then the firmware must flashed into the ROM see the instructions for GenFlash Recalibrate the instrument Front Panel Assembli...

Page 62: ...isconnect the ribbon cable from the connector on the Main Circuit board 3 Carefully peel the keypad off the top cover and pull the ribbon cable through the hole in the cover Clean off any excess adhesive Replacement 1 Start the ribbon cable through the hole in the top cover CAUTION AFTER THE BACKING IS REMOVED DO NOT LET THE KEYPAD TOUCH THE TOP COVER OR ANYTHING ELSE UNTIL IT IS READY TO BE PRESS...

Page 63: ...Disassembly and Replacement Main board Main board Connections Figure 5 23 Main board as Viewed From Bottom of Instrument Figure 5 24 Main board as Viewed From Top of Instrument 5 27 ...

Page 64: ...of the way 8 Connect the wires 4 Figure 5 25 Printer Installation and press them into place with a small screwdriver There is only one way that the connector will fit Look closely at the end of the connector it has a slight D shape or make sure that the side of the connector with the shiny metal contacts showing faces away from the printer and towards the plastic door In the figure above the side ...

Page 65: ...NT FOR LOOSE BOARDS OR CABLES COLD SOLDER JOINTS LOOSE HARDWARE WIRE BITS AND LOOSE OR MISSING SCREWS To perform the optical alignment start with the power off Remove the main cover the mono cover the spill tray and the filter wheel Then turn on the power to the unit The lamp on the Visible only models will remain lit Flashing the Lamp for Alignment Preferred Method Note This method is not availab...

Page 66: ...ethod Mirror Adjustments The four mirrors used in the Spectronic GENESYS 10 spectrophotometer are each unique and must only be replaced by the same mirror at a specific location However all four mirrors mount and adjust in the same manner To tell the difference 1 Mirror A has a Blue dot 2 Mirror B1 has no dot 3 Mirror B2 has no dot and is angled up compared to mirror B1 4 Mirror C has a Red dot Th...

Page 67: ...ign it 3 Rotate the mirror as required until it is properly aligned horizontally 4 Tighten the two mounting screws 5 Tighten or loosen the tilt adjustment screw as necessary until the vertical alignment of the mirror is proper Alignment Sequence The various components of the optical path and their locations are shown in Figure 6 2 In addition to any replacement components which may be required the...

Page 68: ...hile performing these alignment procedures Failure to heed this warning may result in serious personal injury including the potential for severe burns heart failure or death WARNING Never stare directly at the light source or the reflected beam from the light source Always wear UV protection in the form of safety glasses or goggles Failure to heed this warning could result in potential eye damage ...

Page 69: ...2 Lock the tool in the open position by compressing the spring until the pin can be inserted into the locking hole in the tool 3 Position the zero order grating Holder tool so that the pin in the base of the tool can be inserted into the rear screw hole where the limit switch was installed and the hole in the tool base is located over the front screw hole Position the grating arm in the L shaped a...

Page 70: ...nt the Sample Target in position on the Cell Holder Platform so that the rectangular target is facing the light beam Secure the Sample Target with its captive screw Install the Platform with the Sample Target into the sample compartment 2 Align the Sample Mirror C horizontally and vertically until the beam is centered on the Sample Target refer to Mirror Adjustments 3 When the alignment has been c...

Page 71: ...1 Reassemble the unit 2 Set the wavelength for 260nm 3 Use a microcell 331752 000 make sure the cell is perfectly clean 4 Fill the microcell with deionized water 5 Gently tap the cell on a padded surface to ensure that there are no bubbles in the light path 6 Insert the cell in the cell holder and zero the instrument 7 Remove and re insert the microcell in exactly the same position several times 8...

Page 72: ......

Page 73: ...crewdriver Magnetic Phillips head screwdriver 2 bit for 4 Phillips screw Black cloth Consumables 335954 000 Printer paper pkg of 5 rolls 335923 000 Lamp Assembly tungsten Spectronic GENESYS 10 Vis 4062 000 Fuses 335971 000 Tubing Battery B 1 on the schematic Panasonic CR 1220 3 Vdc 12mm x 2mm Least Replaceable Units 335983 000 Analog output 335908 1610S Assy Cable Kit Spectronic GENESYS 10 UVscann...

Page 74: ...r flowcell quartz 331732 000 Cell Cylindrical optical glass 100mm set of 2 331731 000 Cell Cylindrical optical glass 50mm set of 2 331768 000 Cell Cylindrical quartz 100mm set of 2 331769 000 Cell Cylindrical quartz 100mm set of 2 335977 000 Cell Funnel Flowthrough 331709 000 Cell Square optical glass 10mm set of 2 331742 000 Cell Square quartz 10mm set of 2 331773 000 Cell Test tube 1 box of 12 3...

Page 75: ...2 155S Keypad Spectronic GENESYS 10 UV single cell only model 335904 155 Keypad Spectronic GENESYS 10 UV and Spectronic BioMate 3 single cell only model I THINK ONE OF THESE IS INCORRECT 335904 155S Keypad Spectronic GENESYS 10 Vis single cell only model 335901 157S Keypad Spectronic GENESYS 10 Vis 6 cell only model 713 021500 Keypad Spectronic GENESYS 10 Bio 6 cell only 713 021400 Keypad Spectron...

Page 76: ...ENESYS 10 113 731200 Power supply replacement power supply only Spectronic GENESYS 10 701 131600 Power supply replacement with cable and hardware Spectronic GENESYS 10 UVscanning and GENESYS 6 701 131700 Power supply replacement with brackets cable and hardware Spectronic GENESYS 10 UVscanning and GENESYS 6 4001 652 Power Entry module 335988 000 Printer 335989 000 Printer light gray 335901 232 Pri...

Page 77: ...ic GENESYS 10 Analog Output Board 8 45 Figure 8 23 Spectronic GENESYS 6 Spectronic GENESYS 10 UVscanning Block Diagram 8 47 Figure 8 24 Spectronic GENESYS 6 Spectronic GENESYS 10 UVscanning CPU 8 49 Figure 8 25 Spectronic GENESYS 6 Spectronic GENESYS 10 UVscanning Memory and Address Decode 8 51 Figure 8 26 Spectronic GENESYS 6 Spectronic GENESYS 10 UVscanning Printer RS232 and RTC 8 53 Figure 8 27...

Page 78: ...Drawings This page intentionally left blank 8 2 ...

Page 79: ...Drawings 8 3 Figure 8 1 Spectronic GENESYS 10 Wiring Diagram ...

Page 80: ...wings 8 4 This page intentionally left blank Dra ...

Page 81: ...Drawings 8 5 Figure 8 2 Keypad Schematic ...

Page 82: ...wings 8 6 This page intentionally left blank Dra ...

Page 83: ...25 MCS 2 PI O24 P CS6 A2 PIO2 P CS5 A1 PIO3 P CS 3 RTS1 RTR1 PI O19 P CS2 CTS 1 EN RX1 PI O18 P CS1 P IO17 P CS0 P IO16 U ZI PI O26 TX D1 PI O27 RX D1 P IO28 C TS 0 ENR X0 PI O21 RX D0 P IO23 TX D0 PI O22 R TS 0 RTR0 PI O20 TMRIN 1 PIO0 TMRI N0 PI O11 HOLD SRD Y PI O6 ARD Y IN T4 PI O30 IN T3 IN TA1 IR Q IN T2 I NTA 0 PWD PI O31 IN T1 SE LEC T 55 INT0 DR Q1 INT6 P IO13 DR Q0 INT5 P IO12 NMI X2 X1 ...

Page 84: ...wings 8 8 This page intentionally left blank Dra ...

Page 85: ... 12 5 29 12 3 28 19 11 2 18 PCS1 WR RD RD LCS VCC VCC C81 0 1 UF C82 0 1 UF GND C83 0 1 UF VCC GND VCC UCS U2 13 12 3 2 14 7 9 10 11 15 5 4 6 1 U29 3 2 1 A0 A1 A10 A11 A12 A13 A14 A15 A16 A17 A2 A3 A4 A5 A6 A7 A8 A9 DQ 0 DQ 1 DQ 2 DQ 3 DQ 4 DQ 5 DQ 6 DQ 7 CE OE WE VSS VCC A18 AM29F004 BT 90JI A2 A0 A1 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 A16 WE OE VCC CE VCC_1 DQ 7 DQ 6 DQ 5 DQ 4 DQ 3 DQ 2...

Page 86: ...Drawings This page intentionally left blank 8 10 ...

Page 87: ... 4 5 3 2 1 6 7 8 J11 9 8 7 6 5 4 3 2 14 13 12 11 10 1 C122 33UF 20V C123 33UF 20V VCC GND CTS1_232 RXD1_232 RTS1_232 TXD0_232 GND R4IN R3IN R2IN R1IN T4OUT T3OUT T2OUT T1OUT V V VCC C1 C1 C2 C2 T1IN T2IN T3IN T4IN R1O UT R2O UT R3O UT R4O UT ADM208EAR VCC1 GND VCC2 RST I O SCL K X2 X1 DS1302ZN 1 2 3 4 5 6 7 8 9 DE 9P KK100 10 SIP 2MM Tanta lum Tanta lum G10 MAIN BOARD 335901 602 1 REV A SERIAL DAC...

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Page 89: ...ARE7 SPARE8 VCC GND 10K R74 VCC U21 6 5 2 1 3 4 U21 8 9 12 13 11 10 WE OE CS GN D VC C A13 A6 A5 A3 A2 A0 A1 A4 A7 A8 A9 A10 A11 A12 A14 I O1 I O2 I O3 I O4 I O5 I O6 I O7 I O8 uPD43256BGU 70L AB 3 AB 4 AB 5 AB 6 AB 7 AB 8 AB 9 AB10 AB11 AB12 AB13 AB14 AB15 AB16 AB17 AB18 AB19 BH E IOC S IOW IOR MEMCS MEMW ME MR OS C1 OS C2 RES ET DB 0 DB 1 DB 2 DB 3 DB 4 DB 5 DB 6 DB 7 DB 8 DB 9 DB 10 DB 11 DB 12...

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Page 91: ...CC GND Q2 68_OHM 100 MHZ L1 68_OHM 100 MHZ L2 C111 GND VO D20 VCC GND 68K R7 68_OHM 100 MHZ L3 D19 J9 13 1 2 3 4 5 6 7 8 9 10 11 12 U23 5 4 6 2 1 LM311 ADJ OUT IN LM 337T AD820A R DAC7611 CLR LD CS CLK SDI VDD VOUT G ND MMBT3 904 C B E 10UF 16V Tantalum MB R0520 MB RD360 SIP 2MM VIN G ND V V 0V NMA0515S G10 MAIN BOARD 335901 602 1 REV A 30V LCD BIAS POWER SEQUENCING U41 8 U25 7 LCD 0to 19 94V NOTE...

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Page 93: ...4 C17 V12_I N V12_SW V_LAMP MC34166TH U30 3 5 4 2 1 100U H L5 MB RD 360 AD 820A R 74AH C32 74AH C32 74AH C32 LM4040 M MBT3904 C B E Electrolyti c Electrolyti c 10UF 16V Tant al um F EE DBAC K SW IT C H VIN COMP GN D G10 MAIN BOARD 335901 602 1 REV A MGN D MGN D THIS TRACE TOBE ACCESSIBLE 1 CHANGING LAYOUTMAY AFFECT RFIMMUNITY NOTES MGN D 2 C17 IS REQUIREDTO DECOUPLE NOISE FROM PHOTOMETRIC SIGNAL 3...

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Page 95: ...V 12 GND V 12 GND GND GND V 12 R37 10K 0 47 0 5W R36 0 47 0 5W R38 0 47 0 5W R24 0 47 0 5W R28 2 5V D16 3 2 1N 4001 D9 D1 D8 D2 D3 SIN _R SIN _R COS _R COS _R U24 2 1 3 U24 6 7 5 U24 9 8 10 U24 13 14 12 MTP3055 D G S MTP3055 D G S MTP3055 D G S MTP3055 D G S AD 820A R VRE FB VRE FA IOUT1A DB 7 DB 6 DB 5 DB 4 DB 3 DB 2 DB 1 DB 0 DS 1 DS 2 VDD DGN D VRE FD VRE FC R W A B IOU T2A 2B IOUT1C IOUT1B IOU...

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Page 97: ... 4 5 3 2 1 6 7 8 9 J1 3 2 1 4 5 6 TU R_MOTORA TU R_MOTORA TU R_MOTORB TU R_MOTORB FW_MOTOR _V FW_MOTORA FW_MOTORA FW_MOTORB FW_MOTORB FW _P HA SE A TU R_PH ASE A TU R_PH ASE B TU R_PH ASE B S1 S2 RTC_EN Q8 3 1 2 U LN 2803 GN D VC LMP IN 7 OU T1 OU T5 IN 8 IN 1 IN 6 IN 2 IN 3 IN 4 OU T2 OU T3 OU T4 IN 5 OU T6 OU T7 OU T8 U LN 2803 GN D VC LMP IN 7 OU T1 OU T5 IN 8 IN 1 IN 6 IN 2 IN 3 IN 4 OU T2 OU ...

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Page 99: ...V 12 V12_PW R VCC GND GND U9 6 4 7 2 3 V 12 V 12 U33 2 16 8 3 14 13 1 15 11 12 7 4 5 6 10 9 D5 D4 V 12 U11 1 3 2 U11 7 5 6 U11 8 10 9 U11 14 12 13 1K 1 R68 V_DET_SAM V REF 0_5 V_DET_REF V REF 0_5 CH_SAM AD820AR AD820AR AD820AR AD820AR AD820AR DG408BR S8 S7 S3 S2 S1 S4 S5 S6 A2 A0 V GND V D A1 EN LS B MB R0520 MB R0520 LM324A M LM324A M LM324A M LM324A M G10 M AIN BOARD 335901 602 1 REV A AGN D AGN...

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Page 101: ...A 5 GND 10 R55 CTS_MP100 10MH Z Y3 VREF 0_5 C69 C70 D22 D23 D24 D25 D26 REFIN RE FOU T VBIAS XOU T XIN AIN 4N AIN 4P AIN 3N AIN 3P AIN 2N AIN 2P AIN 1N AIN 1P AGN D SCLK DGN D SDI O SDOUT DSYNC MOD E CS DRDY AVDD DVD D ADS1211U VOU T VIN GN D AD1403A N AD 820A R 10UF 16V Tantalum 10UF 16V Tantalum MB R0520 MB R0520 MB R0520 MB R0520 MB R0520 G10 MAIN BOARD 335901 602 1 REV A 2 5V 8 PIN DIP D GND A...

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Page 103: ... 2 3 C15 C16 C14 C13 U34 1 11 19 18 16 17 15 14 12 13 9 8 6 7 5 4 2 3 4 1 LM341T 5 0 GN D IN OU T ULN2803 GN D VCLMP IN 7 OUT1 OUT5 IN 8 IN 1 IN 6 IN 2 IN 3 IN 4 OUT2 OUT3 OUT4 IN 5 OUT6 OUT7 OUT8 1MM RT ANG FPC 1MM RT ANG FPC 1D 1Q 2D 2Q 3D 3Q 4D 4Q 5D 5Q 6D 6Q 7D 7Q 8D 8Q CLK CLR 74A HC273 10UF 16V Tantalum 10UF 16V Tantalum 10UF 16V Tantalum 10UF 16V Tantalum 1D 1Q 2D 2Q 3D 3Q 4D 4Q 5D 5Q 6D 6Q...

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Page 105: ...LI M SHD N VC GN D VS W MI NI FI T J R KK 100 8 Electrolyti c MMBT3906 E C B 10UF 16V Tant al um 10UF 16V Tant al um 10UF 16V Tant al um G10 MAIN BOARD 335901 602 1 REV A MGND MGND GRATING NOTE 2 V_MOT 7 CIRCUITANDW2 BELOW NOTUSEDNOW INTENDEDFORSANYO DENKIMOTOR TUNGSTENLAMP PINS1 2 FLASHLAMP PIN3 WIRE ORG TOLAMP PIN1 PIN4 WIRE BRN TOLAMP PIN2 PIN5 WIRE BLK TOLAMP PIN3 PIN6 WIRE RED TOLAMP PIN4 NOT...

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Page 107: ...4 2 10 1 VCC J13 13 11 12 7 8 10 2 3 4 5 6 9 1 C63 0 1UF C114 0 1UF C113 0 1UF TUR_CAL GRATING_LL GND GND 100 Z2 11 9 14 10 12 13 15 16 8 7 6 5 4 3 2 1 47P F C64 47P F C65 47P F C66 47P F C68 47P F C67 G A3 A2 A1 Y3 Y4 Y2 Y1 A4 74A HC244 G A3 A2 A1 Y3 Y4 Y2 Y1 A4 74A HC244 1D 1Q 2D 2Q 3D 3Q 4D 4Q 5D 5Q 6D 6Q 7D 7Q 8D 8Q CLK CLR 74A HC273 RNSI P10 KK100 13 G10 MAIN BOARD 335901 602 1 REV A CUT OFFP...

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Page 109: ...47P F C118 47P F C117 47P F C116 47P F C115 GND GND GND GND Tantalum Tantalum Tantalum Tantalum KK 156 8 KK 100 2 G10 MAIN BOARD 335901 602 1 REV A 12 12 12 2 COM 5 5 COM COM MGND DGN D CGN D FAN AGN D NOTE C115 C121NOTUSEDNOW INTENDEDFORNOISEREDUCTIONIFNEEDED FANNOT NEEDEDFORG10 DCPOW ERIN 14 OF 14 DC POWER ENTRY AND FILTER Figure 8 16 Main board DC Power Entry and Filter14 of 14 8 33 ...

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Page 111: ... 232 INTERNAL PRINTER FILTER WHEEL MTR TURRET MOTOR RS 232 PORT FLASH LAMP TUNGSTEN LAMP SERIALDAC CONTROL LCD GRA TING MOTOR SIN COS MICROSTEP MULTIPLEXER SAMPLEDETECTORBRD REFDETECTORBRD CPU ANALOG OUTPUT BRD VRAM LCD CNTL 512Kx8 REALTIME 32 768KHZXTAL 18 432MHZCRYSTAL INTERFACE KEYPAD DRIVE KEYPAD GRATING_LL TURRET_CAL POWERSUPPLY 12VDCFAN POW ER 10MHZXTAL A DCONVERTER MUX ADR DATABUS BLOCK DIA...

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Page 113: ...x FFFFF hex 00000 hex SELECTED BY UCS 512K BYTE 128K BYTE 256K BIT 32K X 8 VRAM SELECTED BY MCS0 4M BIT 512K X 8 FLASH EEPROM 256K BYTE 128K BYTE 1M BIT 128K X 8 SRAM SELECTED BY LCS 2 OF 2 MEMORY MAP 335901 602 0 REV A Figure 8 18 Memory Map 2 of 2 8 37 ...

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Page 115: ...7 5 COIL COIL IO1 IO2 JWD 107 5 COIL COIL IO1 IO2 JWD 107 5 S133 7 66 BQ 2 1 G10 DETECTOR BOARD ISLAND ASSEMBLY 335901 604 4 335901 603 4 AND USE S2387 66RFOR VIS DETECTOR BOARD 3 USE HAMAMATSUS1337 66BQ FORUV DETECTOR BOARDASSEMBLY INSTALL W1 B ONLY FORTUNGSTEN CW LAMP 2 INSTALL JUMPERS W1 A AND W1 CFOR XENONPULSE LAMP 1 FOR PULSE LAMP S1 IS ALWAYS 1 SWITCH OPEN NOTES NOTE 2 NOTE 1 ISLAND NOTE 3 ...

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Page 117: ...XA0 MUXA1 MUXA2 1K 1 1K 1 1K 1 A0 A1 A2 COM IN 0 IN 1 IN 2 IN 3 IN 4 IN 5 IN 6 IN 7 INH VDD VEE VSS CD4051 LS B OP177 1N 5819 1N 5819 1N 5819 G10 DETECTOR BOARD 335901 603 1 REV A NOTE INSTALLJUMPERW2FORSAMPLECHANNEL REMOVEJUMPERW2FORREF ERENCECHANNEL SHEET 2 OF 3 R13 R8 R7 R2 R3 R4 R5 R6 D4 D3 D2 R9 R10 R11 U7 U5 R12 Figure 8 20 Spectronic GENESYS 10 Detector board 2 of 3 8 41 ...

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Page 119: ...901 603 1 REV A U5 4 U5 7 U7 16 U6 14 NOTES USE 8 MIL TRACE FOR ALL OTHER SIGNALS 2 PLACE 0 1UF DECOUPLING CAPACITORS C7 C16 CLOSE TO POWER AND GROUND PINS OF ICs 1 PLACE 10UF DECOUPLING CAPACITORS C1 C3 CLOSE TO CONNECTOR J1 U1 2 U2 2 U3 2 U4 2 U7 8 IVC102 CD4051 OP177 U6 9 U7 7 3 USE 25 MIL TRACE TO RUN SIGNALS VCC DGND VA 5 AGND AND VA 10_5 SHEET 3 OF 3 J1 C1 C7 C14 C15 C16 C2 C8 C10 C12 C3 C11...

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Page 121: ...49K_1 1 2 3 1 2 15V 1K_1 1K_1 EX T_DA C_E N DA C_LD RES ET 6 5 2 3 4 1 8 7 0 1UF 50V 0 1UF 50V 0 1UF 50V Tant al um Tant al um Tant al um OP A227 KK 100 10 BN C_RA 2 DAC7611 CLR LD CS CLK SDI VD D VOU T GN D G10 ANALOG OUTPUT BOARD 335901 605 1 SHEET 1 OF 1 REV A J2 U1 C1 C2 C3 R3 C4 R2 C7 C6 C5 R1 R4 D1 J1 U2 Figure 8 22 Spectronic GENESYS 10 Analog Output Board 8 45 ...

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Page 123: ... MULTIPLEXER SAMPLE DETECTOR BRD REF DETECTOR BRD CPU VRAM LCD CNTL 512Kx8 REAL TIME 32 768KHZXTAL INTERFACE KEYPAD DRIVE KEYPAD GRATING_LL TURRET_CAL POWERSUPPLY POWER 10MHZ XTAL A D CONVERTER MUX ADR DATA BUS 256Kx8 24 000MHZ CRYSTAL FLOPPY DRIVE PARALLEL PORT LAMP CHIP CONVERSION SUPER I O OPTIONAL CONTROL FOR 40mJ FLASHLAMP IF NEEDED FOR BOTH 8 5mJ 40mJ LAMPS G10S G6 MAIN BOARD BLOCK DIAGRAM 3...

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Page 125: ...15 U38 38 U38 61 U38 67 U38 92 U38 12 U38 18 U3 8 41 4 2 U38 64 U38 70 U38 89 U36 8 U36 4 U38 21 G10S G6 MAIN BOARD CPU 1 OF 14 335906 602 1 REV A AD 0 7 AD 7 AD 6 AD 5 AD 4 AD 3 AD 2 AD 1 AD 0 WR RD PIO24 LAMP_FAST GND SER_IO IRQ_PARA 69 68 59 60 62 63 65 66 97 98 99 100 1 2 3 72 75 45 46 8 52 53 54 56 76 77 47 14 13 96 95 93 90 87 85 83 81 79 94 91 88 86 84 82 80 78 17 16 73 74 9 10 11 44 58 50 ...

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Page 127: ...5 AD6 AD7 AD0 AD 0 7 AD7 AD6 AD5 AD4 AD3 AD2 AD1 13 14 15 17 18 19 20 21 16 32 12 11 10 9 8 7 6 5 27 26 23 25 4 28 3 31 2 30 1 29 22 24 U40 1 6 4 5 15 11 10 9 7 14 2 3 12 13 U2 UCS VCC 0 1UF C83 0 1UF C81 WR_SIN WR_REFGAIN WR_SAMGAIN WR_COS DAC_LD WR_CNTL WR_TUR FW WR_COLSEL MUXADR VCC GND 12 11 23 25 4 28 29 3 2 30 10 9 8 7 6 5 27 26 13 14 15 17 18 19 20 21 22 24 31 16 32 1 U39 LCS VCC GND WR A0 ...

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Page 129: ...232 RXD1_232 CTS1_232 GND VCC 33UF 20V C123 33UF 20V C122 1 10 11 12 13 14 2 3 4 5 6 7 8 9 J11 Y2 32 768KHZ B1 35MAH J3 GND VCC GND GND C25 0 1UF GND 8 4 1 5 6 7 3 2 U28 C21 0 1UF C20 0 1UF C24 0 1UF C22 0 1UF C23 0 1UF CTS1 RXD1 CTS0 RXD0 RTS1 TXD1 RTS0 TXD0 8 16 23 3 7 20 24 1 2 15 11 9 10 12 13 14 5 18 19 21 6 4 22 17 U15 RXD0_232 RTS0_232 CTS0_232 GND TXD1_232 3 OF 14 335906 602 1 REV A RTC_EN...

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Page 131: ... 000 MHZ LCD CONT ORLLER VCC R3 10 K R2 10 K PIO24 10 11 13 12 9 8 U21 4 3 1 2 5 6 U21 VCC R74 10 K GND VCC VCC PIO24 0 1U F C26 GND 0 1U F C28 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A1 0 A1 1 A1 2 A1 3 A1 4 A1 5 A1 6 A 0 19 CLKOUTA C27 0 1U F 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 91 84 85 86 87 88 89 92 93 32 94 95 96 97 98 99 10 0 1 4 5 6 7 8 9 10 11 76 75 74 73 72 71 70 80 79 78 81 82 33 34...

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Page 133: ...8_OHM 100MHZ R79 33 2 1 R78 33 2 1 V 12 GND L2 68_OHM 100MHZ V 12 VEE R10 1K 1 1 2 6 4 5 U23 12 11 10 9 8 7 6 5 4 3 2 1 13 J9 D19 L3 68_OHM 100MHZ R7 68 K GND VCC D20 VO GND C111 Q2 GN D VCC 0 1 UF C32 SER_IO SER_CLK LCD_DAC_EN DAC_LD RESET VCC GND 6 5 2 3 4 1 8 7 U4 R8 4 87K 1 3 2 4 6 7 U25 VCC C36 0 1 UF GND R33 4 02K 1 R32 210 1 R11 10K 1 3 2 U26 VCC UD3 GND UD0 FLM CL1 VCC UD1 UD2 CL2 VCC R5 6...

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Page 135: ...SLCT SLIN INIT ERR AFD PD2 PD3 PD4 PD5 PD6 PD7 0 1U F C125 0 1U F C124 VCC GND 6 OF 14 335906 602 1 REV A AD 0 7 A 0 10 IRQ_PARA IRQ_FDC CLKOUTA MTR1 DIR DR1 INDEX SUPER_CS SPAR E4 SPAR E5 PC97338VJG A0 A1 A1 0 A2 A3 A4 A5 A6 A7 A8 A9 AEN BADD R0 R TS1 BADD R1 SO UT1 BO UT1 DTR 1 BO UT2 DTR2 A1 2 D0 D1 D2 D3 D4 D5 D6 D7 DRQ 0 DRQ 1 DRQ 2 ID 1 IRSL 1 IO CHRD Y IRQ 10 IRQ 11 IR Q12 IRRX2 ID0 D SR2 I...

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Page 137: ...7 0 5 W R36 0 47 0 5 W 10 K R37 V 12 GND GND GND V 12 GN D V 12 GND 0 1 UF C85 8 2 4 16 15 14 13 12 11 10 9 19 20 1 28 21 27 18 17 5 25 6 23 24 3 7 26 22 U42 R31 1 21K 1 GND GND GND COS SIN COS SIN D11 1N40 01 V12_PWR V12_PWR 1N40 01 D10 0 22 UF C47 3 2 4 6 7 U6 C44 0 22 UF C43 0 1 UF R25 221 1 Q4 R23 510 0 1 UF C48 221 1 R39 510 R40 Q7 Q6 R30 510 R29 221 1 C46 0 1 UF 510 R27 Q5 221 1 R26 0 1 UF C...

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Page 139: ... R_ MOTOR B TU R_ MOTOR B TU R_ MOTOR A TU R_ MOTOR A 6 5 4 1 2 3 J1 9 8 7 6 1 2 3 5 4 10 J6 1 2 3 Q9 3 2 4 5 7 6 8 9 13 12 14 15 17 16 18 19 11 1 U19 3 2 4 5 7 6 8 9 13 12 14 15 17 16 18 19 11 1 U18 2 1 BZ1 TU R_LED_ON TU R_L ED _PW R V12_PWR GND VCC R43 510 GND GND R45 68 K GND VCC VCC TU R_ BOOST FW_ BO OST FW_ PHASEB FW_ PHASEB FW_ PHASEA 11 12 13 5 15 16 17 4 3 2 6 1 8 14 18 7 10 9 U32 TU R_ ...

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Page 141: ...2 3 2 7 4 6 U9 GND GND VCC VEE VO VCC 4 02K 1 R76 1K 1 R35 4 02K 1 R34 R67 1K 1 R64 1K 1 R66 1K 1 R61 4 02K 1 R65 4 02K 1 10K R50 GN D GN D VA 10_5 GND 0 1 UF C96 V 12 0 1 UF C94 0 1 UF C92 V_MUX 3 2 7 4 6 U8 C49 1000PF 3 2 7 4 6 U10 C50 1000PF GN D V 12 V 12 R47 1K 1 VA 5 1K 1 R52 VA 5 VA 5 3 6 4 2 7 U7 1000PF C51 R49 10K R46 1K 1 1K 1 R51 6 4 7 2 3 U12 R48 10K VA 5 0 1 UF C93 VA 5 0 1 UF C95 0 1...

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Page 143: ... DELTA SIGMA A D CONVERTER D26 D25 D24 D23 D22 C70 C69 VREF 0_5 Y3 10MHZ CTS_MP100 R55 10 GND VA 5 C104 0 1UF C103 0 1UF GND 3 2 7 4 6 U13 C105 0 1UF C106 0 1UF GND R54 1K 1 2 1 3 U37 ADC_SCLK ADC_DSYNC ADC_SER_WR 510 R60 R56 510 510 R57 R59 510 R58 510 R53 4 02K 1 12PF C53 C52 12PF 21 20 7 11 10 23 22 1 24 3 2 5 4 6 19 13 14 12 15 16 9 18 8 17 U17 ADC_SER_RD ADC_DRDY VCC GND C107 0 1UF VA 5 VA 5 ...

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Page 145: ... 1 4 U27 3 2 4 5 7 6 8 9 13 12 14 15 17 16 18 19 11 1 U34 C13 C14 C16 C15 3 2 4 5 7 6 8 9 13 12 14 15 17 16 18 19 11 1 U35 RESET WR_REFGAIN WR_SAMGAIN VA 5 1 10 11 12 13 2 3 4 5 6 7 8 9 14 15 16 J7 1 10 11 12 13 2 3 4 5 6 7 8 9 14 15 16 J8 MU XA1_ REF MU XA2_ REF GND VCC VCC GND 9 10 7 18 14 8 1 6 2 3 4 17 16 15 5 13 12 11 U43 R62 324 1 R63 2 49K 1 2 1 3 U14 GND VA 10_5 V 12 V 12 0 1 UF C84 C55 0 ...

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Page 147: ...130 C128 C127 10 K R86 10 K R85 10 K R84 10 K R83 SLCT 2 3 4 8 7 6 5 1 Z3 33 OHM 1 5 6 7 8 4 3 2 Z6 33 OHM 2 3 4 8 7 6 5 1 Z5 33 OHM 1 5 6 7 8 4 3 2 Z4 33 OHM C139 C137 C135 C133 C131 C129 R82 33 2 1 J4 C141 ACK PE BUSY PD0 PD2 PD3 PD1 PD5 PD4 PD7 PD6 ERR VCC AFD PARALLEL PORT 12 OF 14 335906 602 1 REV A SLIN INIT Figure 8 35 Spectronic GENESYS 6 Spectronic GENESYS 10 UVscanning Parallel Port 8 71...

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Page 149: ... AD 5 AD 3 AD 2 AD 0 AD 1 AD 0 AD 7 AD 6 AD 5 AD 4 AD 3 AD 2 AD 1 RD C67 10 00PF C68 10 00PF C66 10 00PF C65 10 00PF C64 10 00PF 1 2 3 4 5 6 7 8 16 15 13 12 10 14 9 11 Z2 100 GND GND 0 1U F C113 0 1U F C114 0 1U F C63 VCC 1 10 2 4 3 5 6 7 8 9 Z1 10 K 3 2 4 5 7 6 8 9 13 12 14 15 17 16 18 19 11 1 U20 GND VCC MUX_ADR2 MUX_ADR1 MUX_ADR0 C62 0 1U F 0 1U F C61 C60 0 1U F 0 1U F C59 C58 0 1U F GND 0 1U F...

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Page 151: ...FLASH LAMP AND DC POWER ENTRY 1 2 3 4 5 6 7 8 J5 V 12 V 12 V12_PWR VCC C9 33UF 20V 6 5 4 1 2 3 J12 33UF 20V C12 C11 33UF 20V 33UF 20V C10 10K R87 R71 10K L7 68_OHM 100MHZ C6 C5 Q1 C56 0 1UF 0 1UF C57 R70 10K 10K R88 Q10 GND V12_FLASH VCC VCC V12_PWR GND GND 335906 602 1 REV A 14 OF 14 LAMP_FAST LAMP_SLOW Figure 8 37 Spectronic GENESYS 6 Spectronic GENESYS 10 UVscanning Flash Lamp and DC Power Entr...

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Page 153: ...to exercise motors independently check gain settings on the detector s as well as calibrate the instrument The Spectronic GENESYS 6 model auto calibrates at power up so there is no option to calibrate it here The instrument can be reset to default by holding down the and ENTER keys at the same time on the instrument key pad while turning on power and then selecting the desired menu option Initiate...

Page 154: ...y Figure 9 3 Communication Error Message 3 Can be caused by a Instrument off or cable disconnected wrong port b Instrument on wrong port Figure 9 4 Communication Error Message Error Establishing Connection 1 Can be caused by a Instrument off or cable disconnected correct port b Instrument on correct port c Instrument off or cable disconnected correct port 9 2 ...

Page 155: ...en told that an analog board is not installed Tells the instrument that an analog board is not installed Single Cell Holder Instrument has been told that it has no turret motor Tells the instrument that it has no turret motor Single Cell Holder Instrument has been told that it has a turret motor Tells the instrument that it has a turret motor LCD Inverse Indicates that the display is dark text on ...

Page 156: ...e Serial Number window is activated allowing entry change of the serial number The serial number will be updated in the instrument when UPDATE SETTINGS is pressed and the checkbox will be deselected Only valid serial numbers are allowed for the instrument type Set Serial Number Not applicable The Serial Number window is inactive and the serial number cannot be changed or entered Button Functions C...

Page 157: ...t Update Settings 1 Updates the instrument with the displayed settings Calibrate 1 Performs a complete wavelength calibration of the instrument 2 Requires the use of a Didymium Filter 3 Must be performed whenever the ROM is changed or Flashed on the Main board 4 Must be performed whenever anything except the lamp in the optical path is adjusted cleaned repaired or replaced 9 5 ...

Page 158: ...n be changed if necessary Left click on Calibrate and follow the screen prompts Figure 9 6 Single Cell Filter Prompt Message Figure 9 7 Cell Filter Prompt Figure 9 8 Final Single Cell Message Filter and turret 1 Allows you to test the Filter motor and the turret motor to check for inoperative binding or skipping motor or gear problems 9 6 ...

Page 159: ...oftware Typical Single Cell Screens Typical 6 Cell Screens Figure 9 9 Single Cell Filter Select Figure 9 10 Cell Filter Select Figure 9 11 Single Cell Filter and turret Window Figure 9 12 Cell Filter and turret Window 9 7 ...

Page 160: ...physically aligning any of the Mirrors 2 Remove the top cover and the Monochromator cover Seek Limit Switch 1 Left click on the Seek Limit Switch button 2 If the limit switch is inoperative the grating arm will just bounce back and forth since it cannot find the limit switch 3 If the system is operating correctly the white beam will be positioned to the left of the exit slit 9 8 ...

Page 161: ... the controls are enabled to change the grating position Do not turn the instrument off while flashing Close the window and then turn off the instrument 5 If the beam is too far from the slit to allow this adjustment then perform the physical mirror adjustment with the ZOGH tool Flash Xenon 1 This control is used to flash the Xenon lamp for optical alignment 2 Click Flash Xenon to start flashing t...

Page 162: ... or ribbon cable A simple way to test the gain settings is to set the instrument wavelength to a low energy point like 850nm and try each gain setting 3 Can be used when the covers have been removed but the sample compartment must be covered with a light tight covering black cloth 4 Displays the energy levels of the Detector board s 5 For the UV model the Sample Energy and the Reference Energy wil...

Page 163: ... Software Figure 9 16 UV Vis Typical Energy Level at 200nm Figure 9 17 UV Vis Typical Energy Level at 1100nm 7 In the Vis model the Reference Energy will be displayed as 1 since there is no Reference Detector board 9 11 ...

Page 164: ...he voltage gain is increased by an increment of 1 the gain steps and gain ratios for the Vis sample detector and UV sample and reference detectors models should be Table 9 1 Voltage Gain Table Step n Gain Step Ratio Energy n Energy n 1 Sample Reference 0 1 0000 8 5250 1 1 3333 11 3667 1 3333 1 43 max 2 1 7778 15 1556 1 3333 1 43 max 3 2 3704 20 2074 1 3333 1 43 max 4 3 1605 26 9432 1 3333 1 43 max...

Page 165: ...902 4 3333 7 14 max 3 28 0000 5 1000 7 14 max 4 40 0000 1 4286 7 14 max 5 70 0000 1 7500 7 14 max 6 280 0000 4 0000 7 14 max 9 If the gain ratios are not within the acceptable range then there is a problem with the Detector board cable or the Detector board or the Main board Lamp 1 Used to reset the Lamp Life value to 0 hours Vis 2 Used to reset the Lamp Life value to 0 0 used UV Figure 9 19 UV Vi...

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Page 167: ...t the current concentration factor FACTOR x Set the concentration factor to x 9999 0 x 9999 0 MODE Report the current acquisition mode A Absorbance C Concentration T Transmittance PRINT Acquire a sample and print the datum PRINT x Print the string x on the printer SAMPLE Acquire a sample and report the datum TRANS Report data in Transmittance if the unit is on the walkup screen it will change also...

Page 168: ... the inverse LCD mode INVERSE 0 Set normal LCD mode INVERSE 1 Set inverse LCD mode LANG Report the instrument language English French German Italian Spanish LANG x Set the instrument language 0 English 1 French 2 German 3 Italian 4 Spanish LCD Report the LCD contrast value LCD x Set the LCD contrast value 0 x 4095 MODEL Report the instruments type as follows Spectronic GENESYS 10 Vis Spectronic GE...

Page 169: ... YY 99 W day 1 W 7 1 is Sunday VER Report the instrument s software version 1 000 Immediate Commands Special control keys that are executed immediately C This command will reset the instruments serial port All characters in both the input and output queues will be lost S Upon receipt of this character all output from the instrument will be suspended Q Upon receipt of this character serial output f...

Page 170: ...Technical Support Bulletins This page intentionally left blank 10 4 ...

Page 171: ...y new AC and DC power cables are required The pinouts for these two cables ARE different for the new XPiQ power supplies as compared to the TPI and Shindengen supplies Part Numbers to Update the Power Supply 701 130600 XPiQ power supply with AC and DC power supply cables for GENESYS 10 BioMate 3 to update a TPI supply to the new XP supply includes one power supply one DC cable one AC cable and one...

Page 172: ...the required cable s per instructions below Failure to do so may result in instrument damage and or personal injury Disconnect power from the instrument before servicing Observe proper ESD protocol Identify which power supply brand make is present in your instrument and carefully follow appropriate instructions below for that particular power supply 1 Shindengen Power Supply refer to Figure 1 a Af...

Page 173: ...nical Support Bulletins b For detail on how to wire power entry module refer to Figure 4 Figure 1 Shindengen Supply Figure 2 TPI Supply Figure 3 XPiQ Supply Figure 4 Power Entry Module for ALL Supplies 11 3 ...

Page 174: ...tyle Hantronix with black wires and the 13 pin connector or the newer style EDT with white wires and the14 pin connector needs to be replaced order the 335901 657S Display Kit Please note that the 335901 657S Display requires software revision 1 103 for the Spectronic GENESYS 10 and 1 001 for the Spectronic BioMate 3 If the unit has software of a lower revision upgrade to the current revision see ...

Page 175: ...uction sheet one Power Supply Bracket one power entry module two fuses one ground label one washer two nuts and four screws Additional Part Numbers available for the Shindengen and TPI power supplies not required to update the Display and the Power Supply 335901 656 TPI Power Supply to replace an existing TPI Power Supply this part number is included in 335901 653S 335901 1610S Replacement Cable K...

Page 176: ...efore servicing Observe proper ESD protocol When installing the following in a Spectronic GENESYS 10 or Spectronic BioMate 3 335901 657 Hantronix display 13 pin header ensure that you use the 335901 1608 display cable Black wires 13 pin connector at P20 end 335901 657 EDT or Hantronix display 14 pin header ensure that you use the 335901 1612 display cable White wires 14 pin connector at P20 end Gr...

Page 177: ...LUTION Release of new firmware 1 103 for Spectronic GENESYS 10 1 001 for Spectronic BioMate 3 PRODUCT AFFECTED All Spectronic GENESYS 10 spectrophotometers of Serial Number D260 were manufactured with firmware 1 103 rev All Spectronic BioMate 3 spectrophotometers of Serial Number D260 were manufactured with firmware 1 001 rev APPLIES TO Repair at customer site Repair at Depot The new firmware will...

Page 178: ...hable ROM APPLIES TO Repair at customer site Repair at Depot There are two part numbers for the Spectronic GENESYS 10 ROM replacement 335901 735S is the basic ROM replacement kit it consists of 335901 735 programmed flash EEPROM with the current firmware 335901 841 installation sheet copy appended to this TSB 335901 740S is the complete ROM replacement kit it consists of 335901 735 programmed flas...

Page 179: ...fore we recommend printing or otherwise recording your test setup screens for greater ease in re programming them Observe proper ESD procedures Use appropriate tools e g PLCC extractor tool A Personal Computer is required to perform this procedure along with a working RS232 serial port and interface cable Part 335942 or equivalent The Spectronic GENESYS 10 Service Diagnostic software Rev 1 40 or h...

Page 180: ...t and ROM Caution Improper insertion will damage the new ROM and possibly the instrument 9 After installation is complete replace and secure the bottom cover and turn the unit right side up 10 Connect Power and turn on the instrument 11 When prompted press F1 or F2 first or second unmarked key from the left under the display for a UV or Visible only model respectively 12 After the unit boots up co...

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