Product Manual for IonPac CS16
Page 37 of 65
5.13.
Isocratic vs. Gradient Elution of Common Cations and Amines at 60 °C
This chromatogram shows the isocratic separation of the common inorganic cations, ammonium, three alkylamines, and three
commonly used additives in the Power Industry: ethanolamine, 5-amino-1-pentanol, and morpholine. The CS16 high
capacity carboxylated analytical column can accomplish this separation using an isocratic, solvent-free eluent consisting of
20 mM methanesulfonic acid. To resolve ammonium from ethanolamine, the column is used at 60 °C. The high temperature
has the added benefit of improving the peak efficiencies and symmetries of the analyte peaks, especially for the alkylamines
(such as peak # 10, trimethylamine). The CSRS 300 is placed outside of the column oven.
Column:
IonPac CS16 3-mm Analytical Column (no guard)
Sample Volume:
25 µL Loop
Eluent:
20 mM Methanesulfonic acid (MSA)
Eluent Flow Rate:
0.5 mL/min
Temperature: 60
°C
SRS Suppressor:
Cation Self-Regenerating Suppressor 300 (2-mm) in
AutoSuppression Recycle Mode (Suppressor placed outside oven)
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS 300 (2-mm)
MMS Regenerant:
TBAOH
MMS Mode:
Displacement Chemical Regeneration (DCR)
Expected Background Conductivity:
< 2 µS
Storage Solution:
Eluent
Analyte mg/L
1.Lithium
0.05
2. Sodium
0.20
3.Ammonium 0.42
4.Ethanolamine 0.50
5.Methylamine 0.50
6. Potassium
0.50
7.Dimethylamine
1.00
8.5-amino-1-pentanol 2.00
9.Morpholine 2.00
10.Trimethylamine 1.50
11.Magnesium 0.25
12.Calcium 0.50
where 1 mg/L = 1 ppm
0
5
1 0
1 5
2 0
2 5
3 0
3 5
0 .1
1 .2
2 .3
µ S
1
2
3
4
5
6
7 8
9
1 0
1 1
1 2
Figure 22
Isocratic Elution of Common Cations and Amines at 60 °C
Document No. 031747-05
© 2010 Dionex Corporation
December 2010
