4
Connecting the EASY-Spray Column Plumbing
Examples of LC Connections to the EASY-Spray Column
Thermo Scientific
EASY-Spray Series Ion Source User Guide
23
To use a nanoViper fitting
1. Insert the nanoViper fitting into the target port and slowly rotate the screw clockwise
until you feel resistance.
2. Using the black knurled fitting tool, tighten the screw clockwise to an angle of
0–45 degrees (1/8-turn).
3. Start operating the system at the desired working pressure and check the backpressure.
4. If the backpressure is too low, check the system for leaks.
For instructions, refer to the LC instrument’s manuals.
5. If the backpressure continues to be too low, return the system to atmospheric pressure.
6. Tighten the screw by as much as an additional 45 degrees. Do not turn the screw beyond
an angle of 90 degrees from where you felt the initial resistance.
Examples of LC Connections to the EASY-Spray Column
This section describes how to connect the one or two plumbing lines from a Thermo
Scientific nanoLC instrument to the EASY-Spray column in a one- or two-column
configuration.
lists the required parts.
Follow the appropriate topic:
•
Connecting the EASY-nLC 1200 to the EASY-Spray Column
•
Configuring the EASY-nLC 1200 Plumbing for Two Columns
•
Connecting an RSLCnano System to the EASY-Spray Column
IMPORTANT
To extend the lifetime of the nanoViper fittings, open and close the
connections at only atmospheric system pressures. Opening and closing connections
at high system pressures can reduce the lifetime of the fittings.
IMPORTANT
To prevent damage to the sealing surface of the nanoViper fitting, do
not overtighten the fitting.
Note
The EASY-nLC 1200 instrument identifies the two solvent lines as “Column Out”
and “Waste In.”
CAUTION
Wear protective gloves and eye wear when handling the solvent lines.
