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Thermo Electron SORVALL RC3BP, Operating Instructions Manual

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RC3 BP™

Operation

4-1

Chapter 4:  OPERATION

This chapter provides the operating procedures for the 

RC3

BP™ Centrifuge.

NOTE

Before using the RC3BP™ for the first time, be sure it is properly installed (see Chapter 2,
Installation). In addition, we recommend that you read all WARNINGS and CAUTIONS, as well as
Chapter 3 to familiarize yourself with the centrifuge controls and features.

Turning the Centrifuge ON

NOTE

Before turning the RC3BP™ ON for the first time, be
sure it is properly installed (see Chapter 2, Installation).

To turn the RC3BP™ ON, find the main POWER switch, located on
a small recessed panel in the upper right corner of the front cabinet
panel, then rock the POWER switch up to the "

I

" (ON) position –

watch the control panel LEDs to be sure that all segments light.

NOTE

If any LED segments do not light, note which ones and
contact a local 

Thermo

 Service Representative. Contin-

ued operation when the centrifuge is unable to display
all run information correctly could mislead an unaware
observer.

During start-up (in addition to lighting all LEDs), the centrifuge
microprocessor performs a short diagnostic routine and the SET
display will appear as follows:

******** SORVALL RC3BP REV X.XX ********

         Self Test in Progress...

If an error is detected, an error message will appear in the SET
display. If no error is found, the SET display will automatically
display the HOME screen that was displayed when the centrifuge
was turned OFF. All OPTIONS selections will also be the same as
when the centrifuge was turned OFF.

Summary of Contents for SORVALL RC3BP

Page 1: ...PN 56311 6 SORVALL RC3BP OPERATING INSTRUCTIONS ...

Page 2: ...RC3 BP Table of Contents PN 56311 6 Issued February 2007 OPERATING INSTRUCTIONS SORVALL RC3BP Lowspeed Refrigerated Centrifuge Thermo Electron Corporation Asheville North Carolina U S A ...

Page 3: ...is manual may contain data in apparent conflict with that provided herein Please consider all data in this manual to be the most current WARNING CAUTION and NOTE within the text of this manual are used to emphasize important and critical instructions WARNING informs the operator of a hazard or an unsafe practice that could result in personal injury affect the operator s health or contaminate the e...

Page 4: ... in personal injury or centrifuge damage Never exceed the maximum rated speed of the installed rotor to do so can cause rotor failure Always reduce derate rotor speed as instructed in this manual whenever the rotor speed temperature combination exceeds the solubility of the gradient material and causes it to precipitate the compartment load exceeds the maximum allowable compartment load specified ...

Page 5: ...3 Installation 2 4 Chapter 3 CONTROLS INDICATORS and DISPLAYS RUN Display 3 2 SET Display 3 3 HOME Screen 3 3 Primary Function Keys and HOME Screen Fields 3 4 MENU Key and OPTIONS 3 7 Numeric Keypad 3 10 START and STOP Keys 3 11 Main POWER and KEYPAD LOCK 3 12 SET Display Advisory Messages 3 13 Problem Conditions 3 13 Terminal Conditions 3 16 Chapter 4 OPERATION Turning the Centrifuge ON 4 1 Rotor...

Page 6: ...Breakers 5 7 Parts Ordering Information 5 7 Service Decontamination Policy 5 7 APPENDIX Warranty Decontamination Certificates List of Illustrations Figure Page 1 1 Parts Location and Identification 1 3 2 1 RC3BP Electrical Requirements 2 2 2 2 RC3BP Dimensions 2 3 2 3 Front Locking Stabilizer Adjustment 2 4 3 1 Control Panel 3 1 4 1 Advanced Feature Option Screens 4 6 5 1 Air Filter Assembly Remov...

Page 7: ...Table of Contents SORVALL Centrifuges ...

Page 8: ... up to 6000 rpm and is capable of producing relative centrifugal force up to 7277g It uses a balanced fan cooled high torque brushless dc drive motor that is designed to deliver optimal performance with smooth quiet operation over its full speed range and long bearing life Directly connected to the motor is a gyro action drive with a square spindle that accepts a variety of SORVALL lowspeed rotors...

Page 9: ...erence KEYPAD LOCK a 3 position keyswitch with a removable key that is designed to limit control panel access Besides allowing full functionality the keyswitch can be set so that run parameters cannot be changed or so that only parameters from program memory may be run in either case greatly reducing the chance for operator error or unauthorized changes Run duration controlled by TIME minutes seco...

Page 10: ... ensure proper performance Automatic diagnostics so that if a system malfunction occurs a message appears in the SET display to alert you of the condition Acceptance of a wide variety of SORVALL lowspeed rotors identified in the set display by rotor name for simple verification Figure 1 1 identifies and locates parts of the RC3BP Figure 1 1 Parts Location and Identification ROTOR CHAMBER CONTROL P...

Page 11: ... Heat Output 3 2 kW Dimensions Width 77 cm 30 3 inch Depth 102 cm 40 inch Height top of control console 119 5 cm 47 inch Length power cord minimum 244 cm 96 inch Additional Depth open door to back wall 34 cm 13 4 inch Height top of open door 166 cm 65 3 inch Height rotor installation liftover 92 5 cm 36 5 inch Diameter chamber opening 56 5 cm 22 3 inch Height vent top of backpack 86 5 cm 34 inch W...

Page 12: ... Quality Control and Run Logging software Also available for use with the Network Computer Package is the optional BarTrace Bar Code Reader Accessory Catalog Number 77014 The Network Computer Package allows for connection of up to 16 SORVALL RC3BP and or RC12BP centrifuges to a single computer network for automatic logging of centrifuge QC RUN and daily run data by the WatchLog Network Plus softwa...

Page 13: ...e information contact Thermo or a representa tive for SORVALL products see back cover Parts Supplied Parts supplied with your RC3BP Centrifuge include 56311 Operating Instructions Manual RC3BP 56312 Condensed Operating Instructions RC3BP 56418 Program Log Pad RC3BP 68025 Wrench Open End 9 16 inch 22001 Preinstallation Kit supplied separately Refer to Table 4 1 Rotor Information on page 4 2 for a l...

Page 14: ...single Ø 220 V 50 Hz 30 A single Ø 208 V 60 Hz 30 A single Ø 230 V 50 Hz 30 A single Ø 220 V 60 Hz 30 A single Ø 230 V 50 Hz 20 A P poly Ø 230 V 60 Hz 30 A single Ø 240 V 50 Hz 30 A single Ø The supply voltage should be checked with a voltmeter then you should verify that the voltage indicated on the nameplate on the back panel is in agreement with the measured line voltage If the measured line vo...

Page 15: ...ortant for the centrifuge to function properly To ensure free air circulation the centrifuge must be positioned so that no air vents are blocked allowing for its physical size see figure 2 2 plus an additional 15 cm 6 inches above the opening on top of the backpack and on both sides of the centrifuge For safety personnel should know that centrifuges routinely deal with high energy levels and could...

Page 16: ...re avoid areas near heat sources for example heating pipes and radiators Also avoid close group ing of centrifuges or other heat producing laboratory equipment Generally the cooler the location the better the operating condi tions will be for the centrifuge The centrifuge is intended for use in 1 a Pollution Degree 2 Envi ronment 2 an installation category II supply circuit and 3 at a maximum alti...

Page 17: ...r Check that the centrifuge does not rock on its four support points the two front stabilizers and the two rear casters see figure 2 3 Read the CAUTION 4 Be sure the POWER switch is set to O OFF then plug in the centrifuge power cord 5 Optional To be certain the centrifuge is working properly turn to the Customer Control Inspection paragraph located in Chap ter 5 Maintenance and perform the proced...

Page 18: ...he SET display toggles between positive and negative for temperature value selection ENTER Places input values in memory Bypasses advanced features without selecting or deselect ing them TEMP Allowschangingdesiredcal culatedsampletemperature RECALL Allows viewing and select ing programmed param eters ROTOR Allows selection of the rotor to be used SPEED Allows changing desired speed rpm orRCF gforc...

Page 19: ...entrifugal Effect ACE indicator lit the RUN display shows the current calculated ACE value ω2 dt When the set ACE value is reached the run terminates and decelerates to a stop The displayed value continues to calculate the centrifugal effect during deceleration to zero to show total accumulation Monitoring alternative parameters If a run is in progress and an alternative run duration key is presse...

Page 20: ... it The SET display is used to input and display basic run parameters to select options or other advanced features and to display advisory messages The display s contents are referred to in this manual as a screen for example the HOME screen described below displays basic run parameters that correspond to the keys below the display The HOME screen pictured above displays currently selected values ...

Page 21: ...roplate carrier use to calculate the different RCF The ROTOR key is not active when a run is in progress NOTE To ensure that the installed rotor will always be correctly identified make it a practice to change the SET rotor name immediately after changing the installed rotor SPEED is pressed to specify a different rotor speed in revolutions per minute rpm or relative centrifugal force RCF also kno...

Page 22: ...otor power or slight mechanical differences including normal wear When START is pressed the realized ACE value begins to accumulate in the RUN display The run will terminate and deceleration will begin when the specified ACE value expressed as ω2 dt value up to 9 99 x 1030 displayed as 9 99e30 is reached After termination the RUN display will continue to accumulate until the rotor stops this run t...

Page 23: ...isplay shows the previous run duration values viewable in each control mode Pressing TIME shows the MIN SEC value at termination the SET display shows the last TIME input value HOLD shows the total MIN SEC value after deceleration to zero After an ACE controlled run ACE shows the total ACE value after deceleration to zero the termination value is in the SET display If the previous run was not cont...

Page 24: ... computer interface package The QC RUN feature will run and document the data from each set of parameters that has been saved in program memory When QC RUN is selected the SET screen will prompt you to prepare for the run and press START to begin the centrifuge will start running program number 1 and systematically run each program in order through program number 9 While the QC RUN is in progress ...

Page 25: ...first ROTOR SPEED TIMED DEG C PROG H6000A 2900 04 00 22 1 H6000A 4000 03 00 22 2 H6000A 3800 03 45 4 3 H6000A 5000 05 30 4 4 H6000A 400 00 00 40 5 While the QC RUN sequence is in progress the centrifuge will output to a connected computer the observed speed and temperature along with the SET speed and temperature for automatic data logging and simple performance verification SLOW START chooses gen...

Page 26: ...o any normal manual entry runs as well as to any recalled program runs specific overtemperature limit settings cannot be saved in run programs See the EXAMPLE at left During a run if the calculated sample temperature in the RUN display goes above the maximum allowable sample temperature the run will terminate a SAMPLE TEMPERATURE OVER LIMIT message will appear in the SET display and an alarm will ...

Page 27: ...rogram memory To eliminate inadvertent loss of existing programs the save run sequence alerts users before overwriting The convenient RECALL key allows browsing through existing programs for selection or reference NOTE If you plan to use the QC RUN feature specific guidelines should be considered when saving parameters to memory see QC RUN on page 3 6 Numeric Keypad The grey Numeric Keypad is used...

Page 28: ...ile the run is in progress the light on the key will be ON The light will remain ON after run termination during deceleration until the rotor stops spinning and door unlocks The red STOP key is used to terminate a run When stop is pressed or whenever a run terminates and deceleration begins the red LED status indicator light on the key is will be ON The indicator light will remain ON until the nex...

Page 29: ... follows The middle position FULL FUNCTION allows use of all keys and features without restriction The upper position RUN SINGLE PROGRAM minimizes func tionality so that set parameters cannot be changed START STOP and CLEAR are the only active keys The lower position RUN ANY PROGRAM makes it so that users may only perform runs using a run parameters recalled from program memory only RECALL the num...

Page 30: ... RUN Temperature display until the door has been opened The centrifuge will not start Open the door if you had not accessed the chamber since the last run make sure the rotor is properly loaded then close the door and press START If the message reappears when START is pressed it indicates a door switch failure In that case remove all sample from the rotor unplug the centrifuge and contact Thermo S...

Page 31: ...as still within 200 rpm of SET speed when the power was restored The automatic restart feature re sumed the run in progress WatchLog Network does not document this occurrence POWER FAILURE RUN TERMINATED A power failure occurred during the run and the rotor speed was not within 200 rpm of SET speed when power was restored disabling automatic restart The run in progress was terminated If power is r...

Page 32: ...The centrifuge will not start This can occur if you change the SET rotor but the SET speed is too high for the newly selected rotor or if you recall a program that was saved based on the use of a different higher speed rotor Enter an rpm or RCF value that does not exceed the maximum allowable for the rotor selected or select a different rotor and press START THE SET SPEED EXCEEDS ROTOR MAX RUN TER...

Page 33: ...r is opened note if the chamber is warm if so the rotor may be too hot to touch Unplug the centrifuge and contact Thermo Service WatchLog Net work record incomplete COMPRESSOR DOOR CLOSED SWITCH FAILURE A run was in progress when the door closed switch opened indicating a circuit failure Remove all sample from the rotor after it stops then unplug the centrifuge and contact Thermo Service WatchLog ...

Page 34: ...icating that the tachometer circuit has failed or that the drive shaft is damaged Wait for the rotor to stop spinning this could take as long as 20 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING WatchLog Network record TACH FAULT UNEXPECTED ISR CALL SERVICE The microprocessor has detected erroneous info...

Page 35: ......

Page 36: ...anel then rock the POWER switch up to the I ON position watch the control panel LEDs to be sure that all segments light NOTE If any LED segments do not light note which ones and contact a local Thermo Service Representative Contin ued operation when the centrifuge is unable to display all run information correctly could mislead an unaware observer During start up in addition to lighting all LEDs t...

Page 37: ... force 1 Mass grams 2 grams rpm Available SWINGING BUCKET H 6000A 5000 7277 25753 10 450 yes HBB 6 5000 7129 28003 10 450 no HLR 6 5000 7205 28003 10 450 no H 6000A M 5000 5186 462 10 450 no with microplates H 2000B 2800 2297 750 10 800 yes H 2000B M 2800 1822 290 10 800 no with microplates HL 2B 3300 2024 20003 5 700 no HBB 12 2900 2462 38203 25 500 no H 4000 5250 7121 25753 10 450 no FIXED ANGLE...

Page 38: ... RCF g force field press SPEED and input the desired value Values in excess of the maximum for the specified rotor will not be accepted the value will continue to blink To change mode from rpm to RCF or from RCF to rpm press SPEED more than once an equivalent value will be calculated based on the maximum radius of the specified rotor and displayed b If you require a change to the TIMED HOLD ACE fi...

Page 39: ...0 03 00 22 2 H6000A 3800 03 45 4 3 H6000A 5000 05 30 4 4 H6000A 400 00 00 40 5 2 Press MENU to access the advanced features screens then press ENTER seven times after first accessing the advanced features until the following screen is in the SET display SELECT SAVE RUN 1 YES O or ENTER NO 3 Input 1 The following screen will appear in the SET display ENTER PROG 00 SINGLE RUNS 1 9 STEP RUNS 10 15 NO...

Page 40: ...s fully seated and locked to the drive spindle Install the rotor cover if any then close the chamber door NOTE Rotor names are not saved or recalled be sure that the installed rotor is correct for the recalled program make sure that the SET rotor identifies the installed rotor 5 If using WatchLog Network run logging software make sure it is running then press START indicator will light The rotor w...

Page 41: ...N P P P PR R R RE E E ES S S SS S S S S S S ST T T TA A A AR R R RT T T T T T T TO O O O B B B BE E E EG G G GI I I IN N N N E E E EN N N NT T T TE E E ER R R R A A A AC C C CC C C CE E E EL L L LE E E ER R R RA A A AT T T TI I I IO O O ON N N N R R R RA A A AT T T TE E E E 0 0 0 00 0 0 0 1 1 1 1 1 1 1 10 0 0 0 1 1 1 1 S S S SL L L LO O O OW W W WE E E ES S S ST T T T E E E EN N N NT T T TE E E ER...

Page 42: ...ram saved to PROG positions 1 9 programs will be skipped if programmed to factory set tings SPEED 400 rpm TIME 00 00 and TEMP 40 QC RUN data will automatically be recorded by the WatchLog Network software so that centrifuge performance with respect to the SET speed and temperature is documented and can be verified NOTE If you plan on using the QC RUN feature consider the following as you save runs...

Page 43: ... no longer be lit SELECT SLOW START This screen allows you to select a starting rotor acceleration rate to 250 rpm that is more gentle than normal maximum accel eration If SLOW START is selected when START is pressed the rotor will accelerate at a more gradual rate up to 250 rpm then transition to normal full acceleration to SET speed After selecting the SLOW START option entering 1 10 selects one...

Page 44: ...that will terminate a run in progress if the calculated sample temperature in the RUN display exceeds SET temperature by more than a specified amount CHANGE OVERTEMPERATURE LIMIT al lows you to specify a maximum allowable sample temperature as it relates to the current SET temperature to create a new overtemperature offset value that will be applied to future changes to set temperature Overtempera...

Page 45: ...s STEP RUN is deselected before START is pressed Because of this the only changes that could be made without deselecting would be if a different step run was recalled from program memory program numbers 10 15 After STEP RUN selection SELECT SAVE RUN will appear in the display For future use step runs may be saved to program memory and can occupy program number 10 15 SELECT SAVE RUN This screen all...

Page 46: ... either store the rotor in a controlled temperature environment such as refrigerator or cold room or precool preheat the rotor in the centrifuge cham ber until the rotor is the same temperature as the sample and the required SET temperature Temperature control needs can vary with the application In many cases the RC3BP Centrifuge will be used to run large volumes of sample for short amounts of tim...

Page 47: ...eration Once a run is completed and the chamber door is opened the vapors or aerosols which have concentrated in the chamber will be released into the laboratory area For this reason when materials which are patho genic toxic or otherwise hazardous in nature are to be run the centrifuge should be located in a biohazard safety enclosure and operated with all appropriate safety precautions An added ...

Page 48: ...uced proportionately Strict adherence to the maximum allowable compartment mass or reduced speed see below is required to prevent rotor failure Read the WARNING on the Safety Information Page in front of this manual If the actual compartment mass total contents as explained above is more than that specified for the rotor the rotor speed must be reduced in proportion to the square root of the ratio...

Page 49: ......

Page 50: ...d be kept clean and wiped routinely to maintain efficient cooling Wash the rotor chamber with a mild nonalkaline dishwashing liquid then rinse and dry with a soft absorbent cloth Use 70 ethanol to disinfect the rotor chamber or a 2 glutaral dehyde solution to sterilize it For general radioactive decon tamination use a solution of equal parts of 70 ethanol 10 SDS and water Follow this with ethanol ...

Page 51: ...n position Also check that the gas spring bracket is not cracked If the chamber door will not stay in the open position or the gas spring bracket is cracked contact your local Service Representative Lubrication All components are prelubricated and require no further lubrica tion The refrigeration unit is hermetically sealed and the ball bear ings in the gyro action drive assembly and the centrifug...

Page 52: ...lter will pop up out of the holder Discard the air filter b While holding one end of the new air filter Cat No 55466 against the foam lower the other end into the holder The spring action of the foam will retract against the air filter and hold it in place 6 Reinstall the air filter assembly making sure the right side of the assembly engages the small bracket on the backpack Once the assembly is e...

Page 53: ...ch option indicator will turn OFF then press MENU to return to the HOME screen 4 Set the run parameters for a commonly used protocol with SPEED controlled by rpm and run duration set to HOLD 5 Press START wait for the SPEED value in the RUN display to reach the specified SET speed then wait an additional 5 minutes for speed to stabilize 6 Using a strobe tachometer check rotor speed through the vie...

Page 54: ...ed temperature area overnight but if that is not pos sible you can precool or preheat the rotor as required Immediately after the test run is performed check test fluid temperature using the same thermometer that was used before the run 1 Choosing a commonly used protocol with the longest run time set the centrifuge run parameters 2 Equilibrate the rotor chamber and the temperature of all rotor pa...

Page 55: ...r while the rotor is spinning and is intended for emergency sample recovery only Read the WARN ING The mechanical door release lever is inside in the back cabinet panel see figure 5 2 To open the chamber door insert a small screwdriver or similar tool through the hole in the grommet and push the door release lever in toward the front of the centrifuge Then while holding the release lever in lift t...

Page 56: ...tion Policy If a centrifuge or rotor that has been used with radioactive or pathogenic material requires servicing by Thermo personnel either at the customer s laboratory or at a Thermo facility comply with the following procedure to ensure the safety of all personnel 1 Clean the equipment to be serviced of all encrusted material and decontaminate it see Care and Maintenance section of centrifuge ...

Page 57: ...cer can decide whether to authorize the rotor s return to a Thermo facility Do not leave a loaded rotor locked inside a centrifuge that requires servicing If with a loaded rotor installed in the cham ber a centrifuge malfunction makes it so that the chamber door will not open by normal means follow the Emergency Sample Recovery procedure found in your centrifuge operating instruc tions manual to g...

Page 58: ...your Thermo representative NOTE United States federal regulations require that parts and instruments must be decontaminated before being trans ported Outside the United States check local regulations If equipment is received at Thermo facilities without a valid RMA Number on the outside of the shipping container and a completed Equipment Return Decontamination Form on file the equipment will be tr...

Page 59: ......

Page 60: ...RC3 BP Appendix APPENDIX ...

Page 61: ...rmo nor to any instrument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument misuse or abuse Thermo reserves the right to change alter modify or improve any of its instruments without any obligation whatever to make corresponding changes to any instrum...

Page 62: ..._________________ INSTRUMENT ____________________________________________________________________ SERIAL NUMBER _____________________________________ ROTOR _________________________________________________________________________ SERIAL NUMBER _____________________________________ PART ______________________________________________________________________________________ PART NUMBER ______________...

Page 63: ...cal hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opinion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Ce...

Page 64: ...l 39 02 95 05 91 NETHERLANDS Tel 31 76 571 4440 SPAIN or POTUGAL Tel 46 8 585 777 50 BELGIUM Tel 32 2 482 30 30 FINLAND Tel 358 9 329 100 RUSSIA CIS Tel 7 095 225 11 15 OTHER ASIA PACIFIC COUNTRIES Tel 852 2885 4613 Other EUROPE MIDDLE EAST or AFRICA Tel 49 6184 90 6940 33 2 28 03 20 00 Visit our web site at http www sorvall com or http www thermo com CANADA or LATIN AMERICA Tel 1 800 522 7746 1 8...

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