4. Operating Instructions
2022-01
HYDROFLEX PLUS No. 30199906 Rev. No. 1.1
35
4.6
Aspirating Modes
To improve wash efficiency and to reduce the residual volume, the manifold must
be positioned so that the aspirating needles are correctly positioned in the wells
for round-bottom, v-shaped bottom, or flat-bottom well microplates.
Normal Aspiration Mode
For round-bottom or v-shaped bottom wells, the aspirating needles are placed in
the middle of the wells. Only one aspiration position can be selected.
Round Bottom Wells
'V' Shaped Wells
Fig. 4.4 Normal Aspirating Positions
Crosswise Aspiration Mode
With flat bottom wells, the instrument can perform crosswise aspiration using two
aspiration positions per well.
The aspirating needles are set at two positions on the bottom of the wells
(front edge and back edge).
Aspirate Y Offset 1
Aspirate Y Offset 2
Front edge
Back edge
Fig. 4.5 Crosswise Aspiration for Flat Bottom Wells
WARNING
TO ENSURE PROPER WASH PERFORMANCE IT IS MANDATORY TO
ADJUST THE HYDROFLEX PLUS TO THE TYPE / MANUFACTURER
OF MICROPLATE OR STRIP-PLATE USED. THIS ALSO APPLIES FOR
ANY PRE-DEFINED PLATE FILE, THAT WILL ALWAYS CONTAIN
AVERAGE PLATE PARAMETERS ONLY, THAT HAVE TO BE
VERIFIED WITH THE CORRESPONDING PLATE TYPE AND IF
NECESSARY CORRECTED BEFORE PUTTING THE
HYDROFLEX PLUS INTO USE.
IF THIS ADJUSTMENT PROCEDURE IS NOT PERFORMED
PROPERLY, THIS MIGHT RESULT IN HIGH LEVELS OF RESIDUAL
VOLUME PER WELL, AS WELL AS INSUFFICIENT WASHING OF THE
WELLS AND MAY SERIOUSLY AFFECT ASSAY PERFORMANCE.
FOR DETAILS ON HOW TO ADJUST THE HYDROFLEX PLUS TO THE
TYPE OF MICROPLATE OR STRIP-PLATE USED, SEE CHAPTER
6 SETTINGS MENU IN THIS MANUAL.
