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TAKARA BIO INC.
URL:http://www.takara-bio.com
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
VI-4. Display of results:
(1) View the View Results screen. (The same time the reaction process is started, the View
Results screen automatically appears. If another screen is open, click the icon “View
Results”).
(2) Click the “Select Graphs” button and the Select Graphs screen appears. From the menu,
select FAM (VT1 amplified curve), TET (Internal control amplified curve) ROX (VT2
amplified curve), and Temperature (the temperature chart graph).
Click and show the "Select Graphs".
When the graphs for FAM, TET, ROX, and
Temperature have been selected at the
initial setting, no entry is required here.
Choose the site and
protocol to be used
and click the [OK] button.
(3) From the Views list, select “Results Table” and enter Sample ID.
Click the
"Results Table".
Enter sample ID.
(4) Click “Analysis Settings” for opening. Click “Usage” next to Ch#4 and select “Unused” from
the pull-down menu. In the Analysis Settings screen, set Manual Thresh Fluor. Units for
Ch#1, Ch#2 and Ch#3 to 100. After entering data in the cells, click the “Update Analysis”
button, and the settings are activated. This value is used as a cut-off value.