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TAKARA BIO INC.
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
URL:http://www.takara-bio.com
○
Judgment flow chart
Judgment of VT1
Displayed on FAM Std/Res as
OOO in the system added with
the sample.
Displayed as POS Displayed as NEG
It was displayed on FAM Std/
Res as OOO in VT1 negative
control.
VT1 contamination is
suspected. Perform the
reaction again after
decontaminating the
place where the reaction
mixture is prepared, and
the apparatus to be used.
VT1 gene positive,
regardless of the result
of internal control (ROX
Std/Res) (whichever it is
POS or NEG).
It was displayed on FAM Std/
Res as OOO in VT1 positive
control.
Internal control is not
amplified, either. There is
a certain problem about
the whole reaction sys-
tem, and reaction and
detection are not per-
formed normally. Reform
the reaction again.
There is no problem
about VT1 detection
system. VT1 gene in a
sample is below a detec-
tion limit.
There is a problem
about VT1 detection
system. A problem is in
primer for VT1 amplifica-
tion, or in a detection
probe, or VT1 positive
control has decom-
posed.
Displayed on TET Std/Res as
OOO in the system added with
the sample.
Displayed as POS Displayed as NEG
Displayed as POS Displayed as NEG
Displayed as POS Displayed as NEG