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TAKARA BIO INC.
URL:http://www.takara-bio.com
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
○
Judgment of VT2
Displayed on TxR Std/Res as
OOO in the system added with
the sample.
Displayed as POS Displayed as NEG
It was displayed on TxR Std/
Res as OOO in VT2 negative
control.
Displayed on TET Std/Res as
OOO in the system added with
the sample.
Displayed as POS Displayed as NEG
Displayed as POS Displayed as NEG
It was displayed on TxR Std/
Res as OOO in VT2 positive
control.
Internal control is not
amplified, either. There is
a certain problem about
the whole reaction sys-
tem, and reaction and
detection are not per-
formed normally. Reform
the reaction again.
Displayed as POS Displayed as NEG
There is no problem
about VT2 detection
system. VT2 gene in a
sample is below a detec-
tion limit.
There is a problem
about VT2 detection
system. A problem is in
primer for VT2 amplifica-
tion, or in a detection
probe, or VT2 positive
control has decom-
posed.
VT2 contamination is
suspected. Perform the
reaction again after
decontaminating the
place where the reaction
mixture is prepared, and
the apparatus to be used.
VT2 gene positive,
regardless of the result
of internal control (TET
Std/Res) (whichever it is
POS or NEG).