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7
TAKARA BIO INC.
URL:http://www.takara-bio.com
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
VI-3. Preparation of reaction mixture:
Enter Run Name
Select FTTC25 in
Dye Set.
Choose the site and protocol to
be used.
Click the [OK] button.
(4) Click the icon “Create Run”, enter Run Name, and select Dye-Set (FTTR25 in Smart
Cycler
®
or FTTC25 in Smart Cycler
®
II ).
Click the “Add/Remove Site” button and the “Select Protocols and Sites” screen appears.
From the menu, select the Site and Protocol to be used.
Click the Add/Romove Sites to display
the [Select Protocol and Sites] screen.
This product detects simultaneously three amplification products, VT1, VT2, and internal
control, within one reaction tube. In order to obtain the right detection result, we recommend
you to perform VT1 positive control reaction, VT2 positive control reaction, and a negative
control reaction altogether.
(1) Prepare the reaction mixture on ice shown below. (Perform in Area 1)
Prepare the mixture except adding template in the tubes of required number plus a few.
Dispense it into Smart Cycler
®
tubes and then add template. The required number means
sample number plus three. This additional three means the tubes which are added with
VT1 positive control or VT2 positive control, or sterilized distilled water in place of template,
respectively.