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14
TAKARA BIO INC.
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
URL:http://www.takara-bio.com
Table 1: Reactions with a sample (Final judgement should be done by referring to
the results of each control reaction)
TET Std/Res (International control)
POS NEG
FAM POS
VT1 Positive
*1
VT1 Positive
*1
Std/Res
(VT1) NEG VT1 Below the detection limit
*2
Judgement impossible
*3
TxR POS
VT2 Positive
*1
VT2 Positive
*1
Std/Res
(VT2) NEG VT2 Below the detection limit
*2
Judgement impossible
*3
Reference table for judgement:
Table 2: VT1 positive control reactions (VT2 negative control)
TET Std/Res (International control)
POS NEG
FAM POS
No problem in VT1 detection
No problem in VT1 detection
*1
Std/Res
(VT1) NEG Problem
*4
in VT1 detection
Judgement impossible
*3
TxR POS
VT2
contamination suspected
*6
VT2 contamination suspected
*6
Std/Res
(VT2) NEG No VT2 contamination
*2
Judgement impossible
*3
Table 3: VT2 positive control reactions (VT1 negative control)
TET Std/Res (International control)
POS NEG
FAM POS
VT1 contamination suspected
*6
VT1 contamination suspected
*6
Std/Res
(VT1) NEG No VT1 contamination
*2
Judgement impossible
*3
TxR POS
No problem in VT2 detection No problem in VT2 detection
Std/Res
(VT2) NEG Problem
*5
in VT2 detection Judgement impossible
*3
Table 4: Negative control (What added sterilization distilled water)
TET Std/Res (International control)
POS NEG
FAM POS
VT1 contamination suspected
*6
VT1 contamination suspected
*6
Std/Res
(VT1) NEG No VT1 contamination
*2
Judgement impossible
*3
TxR POS
VT2 contamination suspected
*6
VT2 contamination suspected
*6
Std/Res
(VT2) NEG No VT2 contamination
*2
Judgement impossible
*3