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33 

STEINDORFF

®

 

 

5. Troubleshooting 

Under  certain  condition,  some  no-fault  factors  will  bring  a  reversible  influence  to  the  instrument’s 

performance. If the problem is happened, please take proper measures according to the follow table. If you can’t 
solve the trouble by the supplied methods, please contact with the sales department of our company. 

PROBLEM 

REASON 

SOLUTION 

I. Optical Part: 

1.  The  illumination  is 
opening, but the field of 
view is dark. 

The plug of the lamp holder is not connected 
into the illumination set 

Connect them well  

The bulb burnt out 

Change a new lamp 

The brightness is too low  

Adjust to a proper position 

The color filter is piled too much 

Minimize the number of the filters 

No use the appointed lamp bulb 

Use the specified halogen Lamp 6V30W 

2. The edge of the field 
of view has shadow or 
the brightness is 
asymmetry 

The nosepiece is not in the located position 

Turn the nosepiece into the position where 

you can hear “clicked” 

the color filter is stopped midway 

Insert deeply 

The phase contrast slider is not located in the 
proper position 

Turn the slider into the “clicked” position  

3. Find dust and stain in 
the field of view 

There are stains on the specimen 

Change a clean specimen 

There are stains and dust on the eyepiece 

Clean the eyepiece 

4. Appear double image 

the size of the aperture diaphragm is too small 

Open up the aperture diaphragm 

5. Resolution problems: 

 

Image is not 
sharp

 

 

The contrast is not 
high

 

 

The detail is not 
clear

 

 

 Don’t obtain the 
phase contrast 
effect 

The nosepiece is not in the center of the light 
path 

Ensure the nosepiece is turned into the 

“clicked” position 

the aperture diaphragm in the view of field is 
opened too large or too small  

Adjust the aperture diaphragm correctly 

The lens (condenser, objective, ocular or 
culture dish) become dirty  

Clean all 

In the phase contrast observation, the bottom 
thickness of the culture dish is more than 
1.2mm. 

Use a the culture dish whose bottom 

thickness is less than 1.2mm 

Use a bright field objective  

Change to the phase contrast objective 

 

The condenser ring is not coincident with the 
objective phase ring 

Adjust the condenser ring to match the 

objective phase ring 

The light ring and the phase contrast kits is not 
centered 

Adjust the bolts to center them 

The objective used is not fit to the phase 
contrast observation 

Please use the compatible objective 

When looking at the edge of the culture dish, 
the phase contrast ring and the light ring is 
deviated each other 

Moving the culture dish until you obtain 

phase contrast effect. You also can demount 

the slider, dail the field diaphragm with the 

direction of “

” 

6. One side of the image 
is unfocused 

The nosepiece is not in the center of the light 
path 

Insure the nosepiece is in the “clicked” 

position 

The specimen don’t place properly 

Place the specimen on the stage correctly. 

The optical performance of the culture dish 
bottom is poor (such as erode figure and soon) 

Please use a regular culture dish 

 

 

Summary of Contents for NYMCS-1701

Page 1: ...ual This manual is written for Inverted Fluorescence Microscope NYMCS 1701 For safety and for keeping the best performance making you familiar with the instrument entirely it is strongly recommended t...

Page 2: ...llation 5 2 2 Installment steps 6 3 Adjustment Operation 11 3 1 Lamp adjustment for fluorescence observation 17 3 2 Reflected observing illumination adjustment 17 3 3 Imaging Operation 19 4 Outfit 32...

Page 3: ...e setting of the voltage switch and the frequency switch on the rear of the power supply unit 7 Always use the power cord provided and make sure that the main switch is moved to O OFF before connectin...

Page 4: ...mpt to use organic solvents to clean the non optical component of the equipment To clean these use a lint free soft cloth lightly moistened with a diluted neutral detergent 3 Do not disassemble any pa...

Page 5: ...ontrast slider Lamp Reflected fluorescence units Condenser Illumination bracket Eyepiece Stage inserted plate Stage Body Nosepiece Objectives 4 10 20 40 Fluorescence power supplier Protection barrier...

Page 6: ...steps Before installing be sure every components is clean do not score any parts or glass surface Keep well with the supplied hexagon wrench When changing the components you will need it again Condens...

Page 7: ...the setscrew and insert the Viewing Head into the body correctly screw down with bolt 2 2 2 Installing eyepiece fig 2 Insert the eyepiece into tube until they are against 2 2 3 Installing condenser s...

Page 8: ...it needn t to adjust in the use process If the ring is not in the center you could adjust by the centering bolt The 10X 20X light ring is worked with the 10X 20X phase contrast objective while the ope...

Page 9: ...used in the inversed microscope is very sensitivity about dust Do cover all the unused holes with turret dust caps to prevent the dust and contamination entering inside When operating use the low magn...

Page 10: ...ly unit and make sure the cord is correctly connected Make sure that the main switch of the power supply is set to O OFF before connecting cables 3 Connect the power cord connector into connector on t...

Page 11: ...fortable adjustment 1 Installing the stage lengthen splint First Screw the fixed bolt on to the splint then mount it on to the stage from right or left below screwing down it until it stay hard 2 Inst...

Page 12: ...t be re ignited for about 10mimutes that is until the mercury vapor inside it has cooled down and condenser to liquid Ensure that the hour counter is reset to 000 00 after replacement of the burner An...

Page 13: ...dicator pointed by the spot on the eyepiece holder shows the scale of the interpupillar distance The range of the interpupillar distance 48 75mm 3 1 5 Mounting Auxiliary Stage fig 4 1 When using mecha...

Page 14: ...ve the objective from the revolving nosepiece position and engage this position in the light path 5 Pull out the field iris diaphragm lever to close the iris diaphragm and push in the aperture iris di...

Page 15: ...making sure that the lamp housing has cooled down A mercury burner seals high pressure gas inside If the burner is used beyond its service life stress may accumulate inside the burner and in the worst...

Page 16: ...diaphragm lever out until the diaphragm comes into the smallest state 3 Use the hexangular wrench to adjust the two field iris diaphragm centering screws alternately to move the image of the diaphrag...

Page 17: ...Selecting the appropriate color filters according your need it became more effective to observe or photography the specimen Especially we suggest using the LBD color filter which can compensate more n...

Page 18: ...usted properly before leaving factory How to adjust tension of the coarse focusing knob Turn the tension adjustment collar While revolving at the direction of the arrow in the figure the tension of th...

Page 19: ...ight ring center and the phase contrast center when changing the objective If having departure you ought to center again If the light ring is centering incorrectly you will fail to obtain the best vie...

Page 20: ...n the control board WELCOME should appear on the LCD screen After 3 seconds the microscope system will be in the preview in the real time automatically when you can snap Adjust microscope s definition...

Page 21: ...ig17 01 Shown on the upper right corner is the Nos of photos that can be taken or the video recording time 0000 means in the current setup mode it still can shoot 0000pcs photos 00 00 00 means in curr...

Page 22: ...press the left key to select Yes and confirm by the menu button If you press No by menu button the photo remains stored on the LCD Microscope head itself 3 3 4 Function menu select With the help of th...

Page 23: ...ess menu button again to save your selection Exit from the setting up after confirmation the model comes back to the snap model Fig 18 02 On video model press menu button as shown the Size then press...

Page 24: ...comes back to the snap model Fig 20 Setup Setting up each item On snap model press button as shown the Size then press the down key to enter the Setup Then press the right key to select as Default Da...

Page 25: ...ey to select Date press the right key once again to select On Photo Off Photo Set Date by up down keys Press menu button to confirm selecting On Photo the present date and time will be indicated on th...

Page 26: ...the SD card inserted Attention Even the protected content will be deleted when formatting cannot be resumed again Select Cancel and press menu button the model comes back to the snap model F AE On mea...

Page 27: ...g on LCD screen On playback mode press menu button to find Delete function press right key and select Current or All use up down keys to delete single photo video or all photos video Press menu button...

Page 28: ...d confirm by the menu button Fig 21 03 Meantime the screen become blue Now we can open the installed driver and find PCCam on the desktop of PC Choose option to preview the real time imaging or take p...

Page 29: ...28 STEINDORFF Preview Take video...

Page 30: ...ion 800 600 of microscope LCD screen so through AV outputting TV can show a sharper picture than on microscope screen Also the microscope shoots pictures as 2560 1920 pixels when playback on TV or PC...

Page 31: ...there are 5colors available green blue black white and red It used to select among colors of the self definition graphics facilitating the clear indication under different color materials If you pres...

Page 32: ...ion Optical system Infinite optical system NYMCS 1701 Reflected light source Excitation units excitation Dichroic mirror Barrier filter Blue Excitation BP460 490 DM500 BA520 Green Excitation BP510 550...

Page 33: ...mm Filter 45mm blue green and ground glass Focusing Coaxial coarse and fine adjustment vertical objectives movement Coarse stroke 37 7mm per rotation Fine stroke 0 2mm per rotation Operation condition...

Page 34: ...solution problems Image is not sharp The contrast is not high The detail is not clear Don t obtain the phase contrast effect The nosepiece is not in the center of the light path Ensure the nosepiece i...

Page 35: ...ulb is going to spoil Change the bulb 3 The power cord have a poor contact Check the power cord and connect them exactly 6 Viewing tube The two eyes field of view is different The interpupillary dista...

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