Operating Manual
31
1300-87076-1A Rev. 1
8.1.3 Calculation
If the
Factor Method
protocol, a modified Beer-Lambert equation is used to calculate concentrations with user-
defined measuring absorbance and factor as follows, with or without baseline correction (optional):
Without baseline correction
: c = A x ԑ / b
With baseline correction
: c = (A - A
Baseline
) x ԑ / b
c
the purified protein concentration in the user-defined factor
A
user-defined
the user-defined absorbance
A
Baseline
the user-defined absorbance at baseline wavelength
ԑ
the user-defined extinction coefficient factor
b
the pathlength in cm
8.2 Standard Curve Method
In this application, users can use self-defined wavelength to establish a standard curve for sample measuring.
The correction wavelength is also a self-defined function (optional).
8.2.1 Overview
In standard curve method, the information tab bar has 5 parts: custom settings tab page, sample data tab page,
standard tab page, table tab page, and graph tab page.
The Custom Setting tab page has 3 user-defined options: analysis wavelength, units and correction wavelength
(Figure 26 and Table 17). The function icons will be activated after inserting the
Analysis Wavelength
,
Units
, and
Correction Wavelength
(optional) field.
Figure 26. Custom Setting Tab Page
Table 17. Custom setting tab page information.
Features
Description
Analysis Wavelength
Self-defined wavelength for measuring samples. User has to insert to activate the
function icons (required).
Units
User can insert their self-defined units (required).
Correction
Wavelength
Self-defined wavelength for bichromatic normalization. This is an optional function and
the default is Off.
Function icons
Information tabs
Insert analysis wavelength
Insert self-defined units
Correction wavelength
on/off (optional)
