Operating Manual
14
1300-87076-1A Rev. 1
4.0 Application: Nucleic Acid
This application will measure the samples absorbance value at 260 nm, which is the peak of nucleic acid
absorbing UV light, to calculate the concentration. The unit is ng/µL. The purity of nucleic acid samples can be
estimated by two absorbance ratios, A260/280 and A260/230.
4.1 Overview of Screen Features
The screen of nucleic acid protocol can be separated into 3 parts: information tab bar, information report area,
and function icons.
The information tab bar has 3 pages: data page, table page, and graph page. The information areas show
different reports on different tab pages.
4.1.1 Data Tab Page
On the data tab page of nucleic acid protocol (Figure 9), the data information parts have the features down
below (Table 8).
Figure 9. Data tab page
Table 8. Data tab page information.
Features
Description
[conc.]
The concentration is calculated from absorbance at 260 nm, and the unit is ng/µL.
A260
Displays the absorbance at 260 nm, which is normalized to a 10 mm pathlength
equivalent.
A260/280
Displays the ratio of absorbance at 260 nm and 280 nm. In dsDNA protocol, when the
ratio is < 1.75, a warning icon will pop up. In RNA, a warning pops up when it is < 2.0.
In ssDNA, a warning pops up when it is < 1.75.
A260/230
Displays the ratio of the absorbance at 260 nm and 230 nm.
Name
The sample name can be inserted here. The default is Sample.
Method
Includes sample types like dsDNA, RNA and ssDNA. The default is dsDNA.
Pathlength
The light path chosen by the pathlength selector will be detected automatically and
length will be shown here.
Baseline Correction
The wavelength for bichromatic normalization is 340 nm. This is an optional function
and the default is On.
Function icons
Information tabs
Insert sample name
Sample data information
Sample method types
Selected pathlength
Baseline correction
on/off (optional)
