6)
When the printer is connected, you can print out the result by pressing [P/C].
Note
Choose cells with the same path length for all blanks, standards and samples of
the analytical method you are using.
For measurements below 325 nm, use quartz cells. Glass or plastic cells are only
usable above 325 nm.
The liquid level must be at least 20 mm in a standard square cell.
10.5 Concentration mode
10.5.1 Determination of the concentration via one point
calibration
The results are calculated with the following equations:
CONC = ABS * CONC
Standard
/ ABS
Standard
1. Press [GoTo WL], select wavelength.
2. Place the blank solution in the sample compartment and close the sample compartment
cover.
3. Press [Auto Zero] to measure the blank solution.
4. Remove the blank solution from the sample compartment.
5. Press [Function]. (Press again, if necessary, to display
Factor/Conc
menu item). The LCD will
display as blow.
6. Press [3] to select Factor/Conc. menu item. Press [Enter].The LCD will display as blow.
7. Press [2] to select
Std. Conc.
menu item. Press [Enter]. The LCD will display as blow.
Input
Std. Conc 1.00
1
．
Std. FACT
2
．
Std. Conc
1
．
Abs/%T/Conc
2
．
0%T
3
．
Factor/Conc
4
．
Lamp Exchange
8. Insert the standard solution into the cell holder or cell changer. Close the sample
compartment cover.
9. Use digital key to enter the concentration of the standard, press [Enter].
10. Insert the sample solution into the cell holder or cell changer. Close the sample
compartment cover.
User’s Manual SP-UV 200 20
Spectrum Instruments