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2.
Press [5], to select
Lamp on/off
menu item. Press [Enter].The LCD will display as blow.
4
.
UV lamp off
1
.
VIS lamp on
2
.
VIS lamp off
3
.
UV lamp on
3.
Press [1], to turn on tungsten lamp. Press [2], to turn off tungsten lamp. Press [3], to turn on
deuterium lamp. Press [4], to turn off deuterium lamp.
10.4 Absorbance / Transmittance / Concentration Measurement
1. Press [GoTo WL], select wavelength.
2. Press [Function]. (Press again, if necessary, to display
Abs/%T/Conc.
menu item).The LCD will
display as blow.
3. Press [1], to select Abs/%T/Conc. menu item. Press [Enter].The LCD will display as blow.
1
.
Abs
2
.
Tran
3
.
Conc
1
.
Abs/%T/Conc
2
.
0%T
3
.
Factor/Conc
4
.
Lamp Exchange
4. Measurement
Absorbance/Transmittance Measurement
1)
Press [1], to select Abs menu item. The LCD will display as blow.
546.0 nm ABS
2)
Place the cell with blank solution in the blank position (position 1) of the cell
holder.
3)
Place the samples or standard solution in the other positions of the cell holder.
4)
Close the sample compartment cover. Pull the cell holder’s rod with the blank
sample in the measurement beam. Measure the blank value by pressing the
[Auto Zero] until display shows “100.0%T” or 0.000Abs.
5)
Pull the cell holder’s rod to the next sample position and read the %T or Abs
value of the samples.
Note
Pay attention that second step of the cell changer is only for reading 0%T. There
are two steps between Position 1 and 2 of the manual cell changer.
Alternatively, if you use a single cell holder, at first put the blank solution in the
light beam and set the value to 100%T or 0Abs by pressing the [Auto Zero]. Then
put the sample or standard cell in the cell holder
User’s Manual SP-UV 200 19
Spectrum Instruments