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Sigma 1-15PK, Operating Manual

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Sigma 1-15PK Operating Manual Download Page 15

 

Version 08/2008, Rev. 1.7 - sb 

 

  page 15 of 75 

English translation 
 
 

1.4 Scope 

of 

Supply 

 

The centrifuge comprises: 

 

• 

1 connecting cable 

depending on the voltage variant 

• 

1 rotor wrench 

Part no. 930 050 

• 

20 ml slushing oil 

Part no.   70 104 

• 

1 tube of grease for load bearing bolts 

Part no.   70 284 

• 

Spare fuses 

Part no.   70106 for 220-240 V 

 

 

Part no.   70112 for 120 V 

 

 
 
 
 

Documentation: 

 
Operating manual incl. 

• 

EU Declaration of Conformity (page 5) 

• 

Equipment Decontamination Certificate (page 65) 

 
 
 

Accessories 

according to your order, our order confirmation, and our delivery note. 
 
 
 
 
 

1.5  Standards and Regulations 

 
Please refer to the enclosed EU Declaration of Conformity (page 5). 

Summary of Contents for 1-15PK

Page 1: ...Operating Manual Refrigerated Centrifuge 1 15PK from serial no 120506 Please retain for later use...

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Page 3: ...trifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Phone 49 0 55 22 50 07 0 Fax 49 0 55 22 50 07 12 E mail info sigma zentrifugen de For service please use our online service request...

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Page 7: ...2 3 Informal Notes on Safety 17 2 4 Safety Instructions for Centrifugation 18 2 4 1 Special Instructions 18 2 4 2 Resistance of Plastics 19 2 5 Prohibited Centrifuging Operations and Hazard Warnings...

Page 8: ...Initial Start Up 29 5 2 1 Switching the Centrifuge ON 29 5 2 2 Opening and Closing the Lid 29 5 2 3 Installation of Rotors 30 5 2 3 1 Installation of Angle Rotors with a Hermetically Sealed Lid 31 5 2...

Page 9: ...ent Lockdown 44 5 3 8 Activating Deactivating the Automatic Lid Opening Function 44 5 3 9 Activating Deactivating the Sound Signal 45 6 Malfunctions and Error Correction 46 6 1 Error Mode 46 6 2 Error...

Page 10: ...4 7 2 Sterilization and Disinfection of the Rotor Chamber and Accessories 54 7 2 1 Autoclaving 55 8 Disposal 56 8 1 Disposal of the Centrifuge 56 8 2 Disposal of the Packaging 56 9 Warranty and Liabil...

Page 11: ...perating the centrifuge In addition the local rules and regulations for the prevention of accidents must be complied with 1 2 Intended Use Centrifuges are power driven machines that separate liquids f...

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Page 13: ...ec 11 h 59 min short run continuous run 10 to 40 C 50 Physical data Depth mm Width mm Height mm Weight kg EMC as per EN 61326 Noise level dBA 575 365 300 41 Class B 58 1 3 1 Ambient Temperature The fi...

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Page 15: ...104 1 tube of grease for load bearing bolts Part no 70 284 Spare fuses Part no 70106 for 220 240 V Part no 70112 for 120 V Documentation Operating manual incl EU Declaration of Conformity page 5 Equi...

Page 16: ...erbindung On Power Marche mise sous tension Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Liaison la terre Erde Earth ground Terre Netzstecker zieh...

Page 17: ...Informal Notes on Safety The operating manual is an integral part of the product Please retain the operating manual throughout the service life of the centrifuge Please hand the operating manual over...

Page 18: ...ion of Accessories page 33 Attention Please consider the maximum permissible gravitational field of 12 000 x g when using the microhematocrite rotor An excess gravitational field will result in an inc...

Page 19: ...arnings In the case of non compliance with the instructions the manufacturer cannot be held liable or subject to any warranty claims Only persons who have read and understood the operating manual in w...

Page 20: ...f Attention Ensure that all repairs are performed only by authorized and specialized personnel see 6 2 9 Service Contact page 49 2 5 1 Special Hazards Do not open the lid when the rotor is in motion D...

Page 21: ...e Life of Rotors an Accessories page 34 2 7 Instructions for Emergency Situations If an emergency arises switch off the centrifuge immediately If in doubt call the emergency doctor Fire fighting measu...

Page 22: ...under the centrifuge from the side Install the transport safety device screw at the bottom panel of the centrifuge Transport the centrifuge within suitable packaging and if at all possible in its ori...

Page 23: ...e weighs approx 41 kg Please retain the packaging for any possible future transport of the centrifuge 4 1 1 Transport Safety Device The transport safety device of the SIGMA 1 15PK consists of a screw...

Page 24: ...ficient time for drying before the centrifuge can be used again 4 2 2 Connection The operating voltage on the name plate must correspond to the local supply voltage SIGMA laboratory centrifuges are un...

Page 25: ...rotary knob 4 Right rotary knob 5 Stop key 6 Lid key 7 Display Fig 5 1 total view of the centrifuge 8 Programming connector at the bottom plate only for service purposes Fig 5 2 Programming connector...

Page 26: ...e operating panel When the centrifuge is switched on all of the operating keys and displays will be illuminated for a short time It is now ready for operation 5 1 1 2 Name Plate 1 Manufacturer and reg...

Page 27: ...display is a hermetically sealed LCD display It is operated by way of two rotary knobs and indicates the operating statuses 5 1 5 Electronic System The microprocessor controlled electronic system ens...

Page 28: ...tors the data transfer and sensor signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as error messages with a number on the speed and rcf display see 6 2 8...

Page 29: ...fuge display then illuminates 5 2 2 Opening and Closing the Lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid...

Page 30: ...se the rotor tie down screw must be loosened by some turns and fastened again This must be done once a day or after 20 cycles This ensures a proper connection between the rotor and the motor shaft The...

Page 31: ...the motor shaft Tighten the rotor tie down screw at 5 Nm using the supplied rotor wrench The rotor can also be used without a cover Slightly grease the rotor and lid seals after cleaning The rotors c...

Page 32: ...l them at one end with putty or by fusion Place the capillary tubes into the recesses of the rotor with the sealed end against the rubber ring Ensure that the capillary tubes fit tightly against the r...

Page 33: ...Always load the opposite compartments of the rotors with the same accessories and filling to avoid imbalance Centrifugation with low capacity For the example an angle rotor 24 x 2 2 ml Fig 5 7 and a...

Page 34: ...observe the instructions on safety and hazards in chapter 2 page 16 5 2 5 Service Life of Rotors and Accessories The rotors and accessories have a limited service life Please perform regular checks at...

Page 35: ...tstart function is used to extend the acceleration time whereas the softstop function is used to extend the deceleration time The current combination is shown on the display The stop key can be used t...

Page 36: ...e display Fig 5 11 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired duration is displayed Press the right rotary knob or t...

Page 37: ...power until the maximum speed of the rotor is reached The start key flashes the message short run is displayed in the time display and the duration of the short run is displayed Fig 5 12 When the sta...

Page 38: ...rotary knob Set will now flash Turn the right rotary knob until the desired value is displayed Press the right rotary knob or the start key to confirm the selected value If this is not done the value...

Page 39: ...f the parameter rcf on the display Fig 5 15 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired value is displayed Press the...

Page 40: ...is not done the value will be automatically reset to the last setting The temperature scale shows the actual temperature in the rotor chamber If the selected temperature set temperature is below the a...

Page 41: ...precools the centrifuge under defined conditions Turn the left rotary knob until run appears on the display Select the option by pressing the right rotary cursor Run will now flash Turn the right rot...

Page 42: ...on unit will stop so that no white frost can form inside the rotor chamber 5 3 5 Rotors The available rotors for the centrifuge can be selected here rotor 12124 in the example This is only possible at...

Page 43: ...ired program number is displayed Empty storage location numbers flash Press the right rotary knob or the start key to confirm the input The settings are now saved under this program number program num...

Page 44: ...symbol Fig 5 23 will flash Now the centrifuge can be started and stopped but the settings cannot be changed Proceed in the same way in order to deactivate the function The current status remains acti...

Page 45: ...at the end of the operation as well as in the event of an error message or imbalance Press the lid key five times and on the fifth time hold it down for approx two seconds Proceed in the same way in o...

Page 46: ...g 5 4 name plate page 26 Mains power switch on Switch on power Lid closed Close lid see 6 2 5 Lid cannot be opened page 46 6 2 2 Centrifuge cannot be started Start key LED not illuminated Power off on...

Page 47: ...see 7 1 6 page 54 If the error occurs again call the service 6 2 7 Emergency Lid Release In the event of a power supply failure it is possible to manually open the centrifuge lid using two stoppers th...

Page 48: ...ges allow to slow down power off on allow to cool down provide better ventilation 46 49 Imbalance error allow to slow down power off eliminate the imbalance 50 59 Lid error press lid key close lid rem...

Page 49: ...se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 8425 Fax 49 0 55 22 50 07 9425 E mail service sigma zentrifugen de from other countries contact our agency in your country All agencies are listed at...

Page 50: ...to intensive UV radiation or thermal stress e g by heat generators 7 1 1 Centrifuge Disconnect the power cord from the socket before cleaning Carefully remove all liquids including water and particul...

Page 51: ...ntamination agent depending on the type of contamination Take suitable precautions for your own safety if there is a risk of toxic radioactive or pathogenic contamination Dry the accessories with a so...

Page 52: ...sible signs Check the material regularly at least once a month for cracks visible damage of the surface pressure marks signs of corrosion other changes Check the bores of the rotors and multiple carri...

Page 53: ...mbalance Glass particles in the centrifuge chamber will cause metal abrasion due to the strong air circulation This metal dust will not only pollute the centrifuge chamber rotor and materials to be ce...

Page 54: ...49 7 2 Sterilization and Disinfection of the Rotor Chamber and Accessories Use commercially available disinfectants such as for example Sagrotan Buraton or Terralin available at chemist s shops or dr...

Page 55: ...ime min max cycles Glass tubes 134 138 3 40 Polycarbonate tubes 115 118 30 40 20 Polypropylene tubes 115 118 30 40 30 Teflon tubes 134 138 3 5 100 Aluminum rotors 134 138 3 5 Polypropylene rotor 12124...

Page 56: ...eclaration of decontamination Please comply with any other applicable local rules and regulations 8 2 Disposal of the Packaging Use the packaging to return the centrifuge for disposal Or Dispose of th...

Page 57: ...ml for e g reaction vials no 15008 15040 incl hermetic aluminium lid no 17849 max radius 10 cm min radius 6 7 cm 14 000 21 913 12126 Angle rotor made of polypropylene 36 x 0 5 0 75 ml e g no 15005 inc...

Page 58: ...te capillary tubes heparinized 1 5 x 75 mm 50 l 200 pcs per pack 17005 Capillary tube sealing putty 10 plates 17024 Reader for use with microhematocritrotor 17029 Card reader for 1 capillary tube 1700...

Page 59: ...hical representation of the rotors shows the maximum and minimum radii of the accessories used If necessary the values must be manually calculated see 11 1 1 RCF page 60 Fig 10 3 Microhematocrite roto...

Page 60: ...n If then the speed or the rotation radius is changed the resulting RCF will be recalculated If the RCF is altered the speed will be adapted accordingly under the consideration of the radius r radius...

Page 61: ...Version 08 2008 Rev 1 7 sb page 61 of 75 English translation Fig 11 1 Speed gravitational field diagram...

Page 62: ...age 63 and the Declaration of Decontamination page 65 serve for maintaining the safety and health of our employees Fill out the forms and attach them when returning centrifuges accessories and spare p...

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Page 69: ...Cl3 90 1 4 1 4 1 1 1 4 Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1 Benzene C6H6 100 3 2 4 1 3 4 4 1 1 Boric acid H3BO3 aqueous 1 3 1 2 1 1 1 Butyl acrylate C7H12O2 100 1 2 4 2 3 4 4 4 1 1 Butyl alcohol...

Page 70: ...Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 1 3 Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 1 3 Hydrogen sulfide H2S 10 1 1 1 1 1 1 1 3 1 1 Iodine tincture of I2 1 4 3 1 1 4 4 1 1 Isopropyl alcohol C3H8O 100 1...

Page 71: ...onate Na2CO3 10 1 1 1 1 1 1 3 Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1...

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Page 73: ...ssor 41 Condensation 24 Condenser 54 Connected load 13 Connecting cable 15 Contamination contamination with harmful substances 20 50 51 Continuous heat resistance 54 Continuous run 27 37 Corrosion che...

Page 74: ...0 31 32 50 Multiple carrier 34 52 Noise level 13 Online service request 3 49 Operating voltage 24 Operational safety 20 51 Overseas shipping 22 Padlock symbol 44 Parameter value characterizing the per...

Page 75: ...Set temperature 40 Short run 27 Slushing oil 15 51 52 Solvents 19 50 51 Sound signal 28 45 46 Spare fuse 15 Spare parts 62 Storage temperature 13 22 Stress corrosion 52 Supply voltage 24 Swing out ro...

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