Siemens Hematek Operating Manual Download Page 4

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Contents

General Guidelines for Optimal Staining

........................................................................................3-3

.......................................................................................................................3-3

Start the Instrument

.................................................................................................................3-3

Prime the Tubing

.....................................................................................................................3-4

Load the Blood Smear Slides

....................................................................................................3-5

Stain the Smears

.....................................................................................................................3-6

Clean the Tubing after Use

.......................................................................................................3-7

Turn the Instrument Off at the End of the Day

.............................................................................3-7

Stain Pak Replacement

....................................................................................................................3-7

Section 4: Specimens............................................................................................... 4-3

Overview

.............................................................................................................................................4-3

Peripheral Blood Smears

..................................................................................................................4-3

Bone Marrow Smears

.......................................................................................................................4-5

Section 5: Maintenance............................................................................................. 5-3

General Cleaning

...............................................................................................................................5-3

Daily Cleaning

....................................................................................................................................5-3

Cleaning the Platen

..................................................................................................................5-3

Cleaning the Stain Tubing and Cannula

.....................................................................................5-4

Emptying the Waste Tank

.........................................................................................................5-6

Weekly Cleaning

................................................................................................................................5-6

Cleaning Drain Troughs and Rear Guide Rail

.............................................................................5-6

Decontamination and Removal from Operation

............................................................................5-9

Section 6: Minor Replacements and Adjustments ................................................. 6-3

Fuse Replacement

............................................................................................................................6-3

Replacement of Light Assemblies

...................................................................................................6-4

Replacement of the Pump Tubing

...................................................................................................6-7

Replacement of the Underplaten Tubing

........................................................................................6-11

Pump Volume Adjustment

................................................................................................................6-14

Check and Correct Instrument Operation Prior to Pump Volume Adjustment

.................................6-15

Adjust the Stain, Buffer, and Rinse Volumes

..............................................................................6-15

Volume and Ratio Determination

.....................................................................................................6-17

Sensing Switch Fingers Adjustment

................................................................................................6-18

Section 7: Troubleshooting ..................................................................................... 7-3

Checklist for Quality of Stained Blood Smears

..............................................................................7-3

Troubleshooting Chart

.......................................................................................................................7-4

Section 8: Service, Supplies, and Replacement Parts .......................................... 8-3

Summary of Contents for Hematek

Page 1: ......

Page 2: ...emens Healthcare Diagnostics Origin United States The information in this manual was correct at the time of printing However Siemens continues to improve products and reserves the right to change specifications equipment and maintenance procedures at any time without notice If the Hematek system is used in a manner not specified by Siemens the protection provided by the equipment may be impaired S...

Page 3: ...em 1 11 Operating Lever 1 11 POWER Light 1 12 LOW STAIN Light 1 12 Power Module 1 12 Line Cord Receptacle 1 12 Fuse Holder 1 13 ON OFF Switch 1 13 Specifications 1 13 Disposal of System Waste and Supplies 1 14 Section 2 Installation 2 3 Overview 2 3 Environmental Factors 2 3 Unpacking 2 4 Instrument Setup 2 4 Level the Instrument 2 4 Plug the Line Cord into an Outlet 2 5 Performance Check Prior to...

Page 4: ...ptying the Waste Tank 5 6 Weekly Cleaning 5 6 Cleaning Drain Troughs and Rear Guide Rail 5 6 Decontamination and Removal from Operation 5 9 Section 6 Minor Replacements and Adjustments 6 3 Fuse Replacement 6 3 Replacement of Light Assemblies 6 4 Replacement of the Pump Tubing 6 7 Replacement of the Underplaten Tubing 6 11 Pump Volume Adjustment 6 14 Check and Correct Instrument Operation Prior to ...

Page 5: ...r 8 7 Supplies and Replacement Parts 8 8 Hematek Stain Pak Modified Wright s Stain 8 8 Hematek Stain Pak Modified Wright Giemsa Stain 8 8 Hematek Cannula Set 8 8 Hematek Pump Tube Set 8 9 Hematek Underplaten Tubing 8 9 Ordering Information for Supplies and Replacement Parts 8 10 Appendix A List of Symbols A 1 Symbols Used With This System A 1 Appendix B Hematek Preservice Checklist B 1 ...

Page 6: ...Contents ...

Page 7: ...veyor Spirals 1 6 Platen 1 6 Slide Drying System 1 7 Slide Drawer 1 7 Waste Tank 1 7 Staining System 1 8 Volume Control Panel 1 8 Solution Pumps 1 8 Pump Tubing and Cannulas 1 9 Sensing Switches 1 10 Electrical System 1 11 Operating Lever 1 11 POWER Light 1 12 LOW STAIN Light 1 12 Power Module 1 12 Line Cord Receptacle 1 12 Fuse Holder 1 13 ON OFF Switch 1 13 Specifications 1 13 Disposal of System...

Page 8: ...Page 1 2 Section 1 Introduction ...

Page 9: ...aten Three sensing switches are triggered sequentially as the slide moves along the platen Each switch activates its respective solution pump which meters and delivers the stain buffer or rinse into the capillary space between the slide and platen After staining and rinsing have been accomplished the slide is dried by a flow of air from a low velocity blower and it is then deposited in a slide dra...

Page 10: ...se as well as a predetermined ratio of stain to buffer volumes in the buffer phase A properly stained slide is the result of an interactive process involving the pump volumes the mixing process and the stain to buffer ratio A minimum ratio of 1 2 is recommended however if the platen is filling properly good mixing is occurring and the stained slides are acceptable under the microscope the stain to...

Page 11: ...ng of the stain and buffer The circular bubble level is a water bubble gauge with an inscribed circle that can be observed through the clear plastic lid of the instrument The level is located on the instrument panel to the left of the reagent pumps directly behind the operating lever It is used to ensure the Slide Stainer is in a level position Levelers The feet under the front corners of the inst...

Page 12: ...with opposing grooves Slides to be stained are fitted separately into the opposing grooves which move the slides across the staining surface When loading slides onto the instrument the side covered by the blood smear is faced to the left of the operator with the feathered edge to the back of the instrument Slides are moved by the conveyor spirals side by side from right to left first at the vertic...

Page 13: ...ore they are deposited in the slide drawer A trough around the perimeter of the platen allows for drainage of used solutions into the waste tank below Slide Drying System The dryer is a blower type fan that runs continuously when the instrument is in operation The airflow serves to cool the mechanical components inside the Slide Stainer as well as to dry the slides Slide Drawer The slide drawer is...

Page 14: ...s The volume of reagent being delivered can be adjusted by rotating the respective control dial clockwise to increase the volume or counterclockwise to decrease the volume Solution Pumps The instrument has three pump assemblies one for each solution Each assembly consists of a pump motor four rollers that are attached to the underside of a pump cap and a pump arm All work together to maintain a co...

Page 15: ...tubing 1 for stain 2 for buffer 3 for rinse Each tube has a clear plastic cuff that fits snugly up to the pump arm and holds the tube in position without slippage The tubing is a special type of rubber that is resistant to leaching and brittleness The special diameters and lengths of the tubing assure precise measurement of solutions Each pump tube is connected to a cannula that is inserted into t...

Page 16: ...mp to run After a specific time delay the pumping motor for the specific reagent is activated The solution pumps are set so that precise volumes of stain buffer and rinse are delivered to their respective areas on the platen Each reagent is delivered through its respective cannula and tubing network to the platen orifice The capillary space between the slide and the platen is then filled with the ...

Page 17: ...three positions of the lever are labeled and their related functions are as follows UNLOCK releases the pressure of the reagent pump arms against the pump tubing LOCK locks the reagent pump arms into their proper position against the pump tubing PRIME provides an override control to allow the pumps to run continuously so the tubes can be primed with solutions and cleared of air bubbles The lever m...

Page 18: ...he circuit and illuminates the light The Stain Pak should be replaced at this time Power Module The power module is located on the rear of the instrument on one side of the module is the panel that contains the line cord receptacle fuse and power switch The power module converts the line current coming into the instrument to 12 volts DC which is the voltage within the instrument Figure 1 8 Electri...

Page 19: ...l and controls all power to the instrument Specifications Power Requirements 100 230 VAC 10 50 60 Hz 0 75 Amps Fuse Rating 250 Volt 1 0 Amps 5mm x 20mm Type T Line Leakage Current 5 milliamperes Testing protocol and allowable limits as specified by the safety standards for laboratory equipment outlined in UL 1262 and CSA 22 2 No 151 Dimensions Depth 43 4 cm 17 1 in Width 47 0 cm 18 5 in Height 19 ...

Page 20: ... local licensed biohazardous waste disposal firm for assistance The principal wastes associated with the use of the Hematek Slide Stainer are pump and underplaten tubing effluents from the staining operation and the container for stain buffer and rinse Slides with human specimens control materials and all reagents should also be handled and disposed of in accordance with the prevailing regulations...

Page 21: ...ing 2 4 Instrument Setup 2 4 Level the Instrument 2 4 Plug the Line Cord into an Outlet 2 5 Performance Check Prior to Installing Tubing 2 5 Install the Pump Tubing 2 6 Install the Stain Pak 2 9 Inspect Waste Tank and Slide Drawer 2 10 Familiarize Yourself with the Instrument 2 10 Check Pump Volumes 2 10 ...

Page 22: ...Page 2 2 Section 2 Installation ...

Page 23: ...tal Factors As with all sensitive electronic instruments prolonged exposure to excessive humidity and temperature should be avoided Temperature should be held relatively constant to obtain the highest degree of operating stability The ambient temperature range for operating the instrument is 18ºC to 30ºC 64ºF to 86ºF The ambient operating humidity range is 20 to 85 relative humidity Place the inst...

Page 24: ...rton for further use If the instrument ever needs to be shipped the shipping carton will afford the best protection 4 After the instrument has been unpacked place it on a firm level work surface in the designated work are by lifting the instrument by its frame Instrument Setup Complete the following procedures to ensure proper installation and performance of your Slide Stainer Level the Instrument...

Page 25: ...aste trough Plug the Line Cord into an Outlet CAUTION Be sure the outlet supplies the proper voltage for your instrument Refer to the rating label located on the rear of the instrument to determine the proper voltage rating Plug the appropriate end of the line cord into the instrument and the other end into an appropriately grounded AC electrical outlet Performance Check Prior to Installing Tubing...

Page 26: ...nd you are able to observe the pump cap rotating 6 If the instrument functions properly in these steps continue with the instrument setup procedures 7 If a problem occurred contact the your local technical support provider or distributor Refer to Section 8 Service Supplies and Replacement Parts for more information Install the Pump Tubing 1 Remove the three cannulas and pump tube sets from their p...

Page 27: ...e left and push the tubing through until the plastic cuff is flush against the pump arm Figure 2 3 Threading Tubing through Pump Assembly NOTE If you encounter difficulty in threading the tubing through the pump housing lift the operating lever to PRIME for just a few seconds This will cause the rollers inside the pump housing to rotate slightly and relieve the interference Then return the operati...

Page 28: ...ing 9 Be sure that at least 7 mm 0 25 in of tubing is connected to the nipple for a secure connection Figure 2 4 Attaching Pump Tubing to Nipple NOTE If it is difficult to connect the tube to its nipple use forceps or hemostats to grasp and attach the tubing CAUTION Take care to not damage the tubing when using forceps or hemostats ...

Page 29: ...at the rear of the instrument 2 Make sure the carton is all the way down and resting on the tray at the bottom of the well The carton should be level when properly installed Figure 2 5 Installing the Stain Pak 3 Insert the appropriate cannula into its respective bottle by puncturing the center of the indentation on the bottle Figure 2 6 Installing Cannula into Stain Pak Bottle ...

Page 30: ... waste tank and slide drawer for proper positioning below the platen Each should be pushed completely into its respective cavity in the front of the instrument so it is flush with the control panel on the right front corner Familiarize Yourself with the Instrument Before beginning normal instrument use carefully review Section 3 Operating Instructions and Section 5 Maintenance to become familiar w...

Page 31: ... 3 General Guidelines for Optimal Staining 3 3 Operating Procedures 3 3 Start the Instrument 3 3 Prime the Tubing 3 4 Load the Blood Smear Slides 3 5 Stain the Smears 3 6 Clean the Tubing after Use 3 7 Turn the Instrument Off at the End of the Day 3 7 Stain Pak Replacement 3 7 ...

Page 32: ...Page 3 2 Section 3 Operating Instructions ...

Page 33: ...pump tubing and underplaten tubing on a routine basis Refer to Replacement of the Pump Tubing on page 6 7 and Replacement of the Underplaten Tubing on page 6 11 for recommended frequency Check the alignment of the sensing switch fingers as instructed in step 5 of Check and Correct Instrument Operation Prior to Pump Volume Adjustment on page 6 15 Operating Procedures CAUTION Use Hematek Stain Paks ...

Page 34: ... the tubing then release the pump arm It may also be helpful to pinch the tubing several times with the fingers pinching in the area between the cannula and the pump Figure 3 1 Priming the Tubing 2 Release the lever which will return automatically to the LOCK position CAUTION Always wipe the platen from right to left Damage to the position or shape of the sensing switch fingers may result from not...

Page 35: ...nsure optimal results on the specimen slides 2 Mark blank or old blood smear slides as priming slides for example label them with the name PRIME You can reuse these priming slides CAUTION Slides must be inserted into the spiral grooves so they are parallel to the slide loading lines inscribed on the platen If slides are not placed correctly in the spiral grooves breakage can occur 3 Load two to fo...

Page 36: ... switch which activates its respective solution pump as long as a slide is detected The solution pumps meter and deliver the stain buffer and then rinse into the capillary space between the slide and platen NOTE If the reagents overflow as opposed to filling the capillary space between the platen and slide or if you obtain improper staining results the reagent pumps may need adjustment Refer to Pu...

Page 37: ...er to the UNLOCK position 3 Turn the instrument off Stain Pak Replacement If the LOW STAIN light illuminates when the instrument is first turned on or while slides are being processed a new Hematek Stain Pak is needed Follow these steps to replace the Stain Pak CAUTION After replacement of the Stain Pak the instrument must always be primed to remove any air bubbles that may be present 1 Remove the...

Page 38: ...Page 3 8 Section 3 Operating Instructions ...

Page 39: ...Section 4 Specimens Page 4 1 Section 4 Specimens 4 3 Overview 4 3 Peripheral Blood Smears 4 3 Bone Marrow Smears 4 5 ...

Page 40: ...Page 4 2 Section 4 Specimens ...

Page 41: ...or against the skin of the patient Protect blank slides from moisture and high humidity as well as contamination by dust flies and other insects Store the slides covered in a cool dry place Never use oxalated or heparinized blood for making blood smears EDTA is the anticoagulant of choice Protect blood smears from excessive heat such as radiators and ovens water splatters and high humidity Use the...

Page 42: ...ulate there This may mechanically effect the distribution of the cell types Since frosted end glass slides limit the spreading area extra care is needed when preparing a blood film Figure 4 1 Making a Blood Smear 4 Allow the smear to dry thoroughly before staining See Section 3 Operating Instructions for staining instructions After staining the slide take the following precautions Examine stained ...

Page 43: ... 2 Place the slide onto the Hematek Slide Stainer and stain according to instructions in Section 3 Operating Instructions 3 Remove the slide after it has been stained and examine it under high power dry for the staining quality Do not use any oil on the slide for this examination 4 If the slide is under stained place it on the slide stainer again and stain it a second time In rare instances a thir...

Page 44: ...Page 4 6 Section 4 Specimens ...

Page 45: ...e 5 3 General Cleaning 5 3 Daily Cleaning 5 3 Cleaning the Platen 5 3 Cleaning the Stain Tubing and Cannula 5 4 Emptying the Waste Tank 5 6 Weekly Cleaning 5 6 Cleaning Drain Troughs and Rear Guide Rail 5 6 Decontamination and Removal from Operation 5 9 ...

Page 46: ...Page 5 2 Section 5 Maintenance ...

Page 47: ...ant to empty the waste tank at the end of each day Cleaning the Platen After staining a large number of blood smears precipitated stain solution tends to accumulate in the mixing grooves of the platen This precipitate must be removed at regular intervals Daily cleaning of the platen is imperative after each run is optimal Cleaning is especially important if bone marrow slides have been stained bec...

Page 48: ...ortion of the slide to the front rail If allowed to accumulate slide breakage may occur 4 Thoroughly clean the mixing grooves and the front guide rail Cleaning the Stain Tubing and Cannula The tubing carrying the stain should be cleaned at least once daily or after each run as described in the following procedure 1 Remove the stain buffer and rinse cannulas from the Stain Pak bottles NOTE The buff...

Page 49: ...platen 3 After the stain tubing has been cleaned remove the cannula from the methanol and continue to prime until the tubing is emptied of all methanol CAUTION Always wipe the platen from right to left Damage to the position or shape of the sensing switch fingers may result from not following recommended cleaning practices Check the position of the fingers after cleaning as described in step 5 of ...

Page 50: ...e waste tank with water and reinstall it into the instrument Figure 5 3 Removing the Waste Tank Weekly Cleaning Clean the drain troughs and the rear guide rail on a weekly basis Cleaning Drain Troughs and Rear Guide Rail The back and front drain troughs of the stainer function as drains for excess stain and residues from the reagents that may accumulate and interfere with proper drainage The recom...

Page 51: ...ws 4 Carefully raise the circuit board cover so the back trough is completely exposed and easily accessible Figure 5 5 Raising the Circuit Board Cover 5 Move the panel up and back out of the way The circuit board cover is connected to the instrument by the two connectors of the LOW STAIN and POWER lights Rotate the panel carefully so the connectors are not pulled loose ...

Page 52: ... the back and front troughs to remove the entire excess residue Figure 5 6 Cleaning the Back Trough 8 Clean other exposed areas that might be accidentally stained including the rear guide rail in the same careful manner NOTE Ensure that you do not scratch the platen 9 Return the circuit board cover to its normal position between the circuit board panel and the back edge of the screws and tighten t...

Page 53: ... pump tubing and discard it in an appropriate waste container 3 Lift the used Hematek Stain Pak carton out of the well at the rear of the instrument 4 Follow steps 3 through 8 of the Replacement of the Underplaten Tubing on page 6 11 to turn off the instrument and remove the underplaten tubing Discard it in an appropriate waste container 5 Clean the platen with methanol always wiping from right to...

Page 54: ...Page 5 10 Section 5 Maintenance ...

Page 55: ...of Light Assemblies 6 4 Replacement of the Pump Tubing 6 7 Replacement of the Underplaten Tubing 6 11 Pump Volume Adjustment 6 14 Check and Correct Instrument Operation Prior to Pump Volume Adjustment 6 15 Adjust the Stain Buffer and Rinse Volumes 6 15 Volume and Ratio Determination 6 17 Sensing Switch Fingers Adjustment 6 18 ...

Page 56: ...Page 6 2 Section 6 Minor Replacements and Adjustments ...

Page 57: ... replacements other than those given in this section or if any procedure appears to be too complex refer to Supplies and Replacement Parts on page 8 6 for instructions on service for your instrument Fuse Replacement Use the following procedure to replace the instrument line fuse for all voltage instruments The fuse is 1 0 amp 5mm x 20mm Type T Part No 40151108 1 Turn the instrument off and remove ...

Page 58: ... 8 Service Supplies and Replacement Parts A spare fuse located in the small enclosed compartment in the fuse holder is shipped with the instrument Push the fuse out of the compartment using a small screwdriver 6 With the flat side up return the fuse holder to its position in the instrument 7 Firmly press the fuse cover until it snaps into place and is flush with the power module plate Replacement ...

Page 59: ...s that are inserted through the notches in the back of the circuit board cover Figure 6 3 Location of Thumbscrews 4 Lift the panel from the front wall exposing the printed circuit board and the connectors for the LOW STAIN and POWER lights Figure 6 4 Raising the Circuit Board Cover ...

Page 60: ...t come out completely then pull on the connector wires to remove them from the connector Figure 6 5 Removing the Connector Wires 6 Unsnap the burned out light assembly from the circuit board cover by pinching together the two plastic retaining tabs on the light assembly If the tabs are too stiff use a screwdriver to press against one side then push that edge partially through the opening Repeat wi...

Page 61: ...n top of the screw heads before tightening 12 Replace the line cord into the AC electrical outlet and turn the instrument on to check the light operation Replacement of the Pump Tubing If any one of the three pumps fails to deliver the proper amount of solution at the adjusted volume setting it is recommended that all pump tubes be replaced Regular flushing of the stain tubing with methanol prolon...

Page 62: ...ts 3 Push the operating lever down to the UNLOCK position 4 Disconnect each pump tube from its cannula and from the recessed nipple located in the wall in front of the pumps Figure 6 8 Removing Tubing from Cannula Figure 6 9 Removing Tubing from Nipple ...

Page 63: ...priate waste container Figure 6 10 Removing Tubing from Pump Assembly 6 Remove the new tubes from the Hematek Pump Tube Set Each tube is numerically coded to correspond with the numbers shown on the Stain Pak 7 Attach the coded end of the new tube to its respective cannula a Replace the cannula if it appears damaged or bent using the Hematek Cannula Set PN 4483 b If you are re using any cannulas m...

Page 64: ...culty in threading the tubing through the pump housing lift the operating lever to PRIME for just a few seconds This will cause the rollers inside the pump housing to rotate slightly and relieve the interference Figure 6 11 Inserting Tubing into Pump Assembly 9 Connect the tube to its proper recessed nipple NOTE If difficulty is encountered in connecting a tube to its nipple use forceps or a hemos...

Page 65: ...bing The underplaten tubing is the tubing between the spout under the platen and the nipple behind the circuit board The buffer and rinse tubing should be replaced after approximately ten Stain Paks have been used The stain tubing needs to be changed more frequently especially if it is not regularly flushed with methanol Depending on usage and cleaning patterns the stain tubing may need to be chan...

Page 66: ...efully rotate the panel up and back The circuit board cover is connected to the instrument by the two connectors of the LOW STAIN and POWER lights Rotate the panel carefully so the connectors are not pulled loose 7 Disconnect the LOW STAIN and POWER lights from the circuit board as directed in step 5 of Check and Correct Instrument Operation Prior to Pump Volume Adjustment on page 6 15 in this sec...

Page 67: ...nect the stain tubing from the platen by pulling the tubing free from behind the circuit board and then disconnect it from the spout under the platen Figure 6 15 Removing the Underplaten Tubing 11 Select one of the new sections of underplaten tubing and connect it to the stain tubing nipple behind the circuit board Make sure at least 7 mm inch of tubing is connected onto the nipple 12 Thread the t...

Page 68: ...oard panel and the back edge of the screws and tighten the two thumbscrews Be sure the panel is not resting on top of the screw heads before tightening Pump Volume Adjustment The Hematek Slide Stainer is designed to produce stained slides of consistent quality in a continuous process This is accomplished by having a fixed length of time in each of the three phases of stain buffer and rinse as well...

Page 69: ...ating the pumps at the proper time The stain pump should be activated after approximately of the slide width has passed over the stain orifice The buffer pump should be activated after about 1 8 inch of the slide has crossed over the buffer orifice The rinse pump should be activated just as the leading edge of the slide crosses over the rinse orifice 7 If the starting times for each phase are not ...

Page 70: ...tween the platen and the slide c If the slide is receiving an inadequate amount of stain adjust the control knob clockwise d If overflow of solution is visible on either side of the slide or an excess of stain is visible hanging over the front beveled edge of the platen adjust the stain control knob counterclockwise The adjustment is correct when proper fill has been achieved on two consecutive sl...

Page 71: ...least three blood smears to ensure that the capillary spaces between the platen and slide fill completely with stain at the stain area and with stain buffer mixture just before the rinse area NOTE Occasional small voids may occur on the slide after proper filling These voids are acceptable and will not adversely affect stain quality Do not try to compensate for small voids by increasing the stain ...

Page 72: ...ree tubes to their appropriate spouts and replace the waste tank Sensing Switch Fingers Adjustment The sensing switch fingers must be properly positioned in order to accurately activate the reagent pumps If the reagents are not being dispensed check the location of the fingers Not following recommended cleaning practices may force the sensing switch fingers out of adjustment Use the following proc...

Page 73: ...of the platen without touching the bottom If it is not bend the finger slightly forward or backward as necessary as directed below The sensing switch should be activated before the finger is lifted over the top surface of the slide 6 Lay a slide lengthwise along the back edge of the platen and slowly push the slide from right to left past the sensing switch finger listening for a slight click as t...

Page 74: ...wire no more than 7 mm 1 4 in past perpendicular Figure 6 19 Adjusting the Sensing Switch Finger 8 Verify the final adjustment of each of the sensing switch fingers by processing several slides across the platen as in Step 5 of Check and Correct Instrument Operation Prior to Pump Volume Adjustment on page 6 15 If these adjustments do not result in correct timing contact your local technical suppor...

Page 75: ...Troubleshooting Page 7 1 Section 7 Troubleshooting 7 3 Checklist for Quality of Stained Blood Smears 7 3 Troubleshooting Chart 7 4 ...

Page 76: ...Page 7 2 Section 7 Troubleshooting ...

Page 77: ...blood smear too thick too thin or spread unevenly Does the feathered edge of the smear end at least 1 8 inch from the end of the slide Was the blood smear thoroughly dry before staining Are the slides clean Even new slides are not necessarily clean Are the slides flat Variable thickness of a given slide may cause uneven staining Have the Hematek Stain Pak containers been vented Venting is necessar...

Page 78: ...ing the Stain Paks contact your local technical support provider or distributor Symptom Possible Cause Corrective Action Unit fails to turn on when the main instrument power switch is turned ON Instrument not plugged into wall outlet or instrument Ensure line cord is plugged into outlet and instrument Line fuse is blown Unplug the unit and replace the fuse Refer to Fuse Replacement on page 6 3 Cir...

Page 79: ... on page 6 4 Light switch is not operating correctly Contact your local technical support provider or distributor for information on adjusting the switch Switch or associated circuit is defective Contact your local technical support provider or distributor Improper mixing action between stain and buffer on platen Instrument is not properly leveled Adjust the front feet of the instrument so the bub...

Page 80: ...ot been achieved Refer to Volume and Ratio Determination on page 6 17 Stain volume is adjusted too high Increasing stain volume may decrease stain intensity Refer to Pump Volume Adjustment on page 6 14 Rinse volume is too high Refer to Volume and Ratio Determination on page 6 17 Deteriorated or outdated Stain Pak Replace Stain Pak ...

Page 81: ...e 8 3 Siemens Offices Worldwide 8 4 Returning the Instrument for Repair Exchange Replacement or Loaner 8 7 Supplies and Replacement Parts 8 8 Hematek Stain Pak Modified Wright s Stain 8 8 Hematek Stain Pak Modified Wright Giemsa Stain 8 8 Hematek Cannula Set 8 8 Hematek Pump Tube Set 8 9 Hematek Underplaten Tubing 8 9 Ordering Information for Supplies and Replacement Parts 8 10 ...

Page 82: ...Page 8 2 Section 8 Service Supplies and Replacement Parts ...

Page 83: ...mstances If the problem cannot be solved by performing the steps described in the Troubleshooting Chart on page 7 4 If additional assistance is required concerning an instrument or reagent problem If the problem is beyond the scope of this manual Before calling for service copy and complete the Hematek Preservice Checklist in Appendix A For Service To contact the legal representative for Siemens w...

Page 84: ...ge 8 4 Section 8 Service Supplies and Replacement Parts Siemens Offices Worldwide www siemens com diagnostics Siemens Healthcare Diagnostics Pty Ltd 885 Mountain Highway Bayswater Victoria 3153 Australia ...

Page 85: ...he ON OFF switch to the OFF position 2 Follow all steps in Decontamination and Removal from Operation on page 5 9 3 Pack the instrument in the original bag and container or as instructed by your local technical support provider or distributor 4 Seal the container securely 5 Return the container to your local technical support provider or distributor NOTE If Siemens does not receive your defective ...

Page 86: ...ident or if you feel unwell seek medical advice immediately show the label where possible Hematek Stain Pak Modified Wright Giemsa Stain This is a modified Wright Giemsa stain for laboratories that prefer a Giemsa stain component for preparation of their blood smears This Stain Pak contains a modified polychrome methylene blue eosin stain based on the original stain proposed by Romanowsky Also sup...

Page 87: ...ten Tubing is available for periodic replacement The tubing set consists of three tubes of equal diameter and length and therefore are not specific to a reagent Ordering Information for Supplies and Replacement Parts To order the following Hematek brand products contact your local technical support provider or distributor Part Number Description 4481 Hematek Stain Pak Wright s Stain 4405 Hematek S...

Page 88: ...Page 8 8 Section 8 Service Supplies and Replacement Parts ...

Page 89: ...tious specimens in Protection of Laboratory Workers from Infectious Disease Transmitted by Blood Body Fluids and Tissue 2d edition Approved Guideline 1997 Document M29 A National Committee for Clinical Laboratory Standards NCCLS This document contains complete information on user protection and it can be used as reference material for instructions on laboratory safety These symbols are used for bo...

Page 90: ...n Identifies Hematek buffer Identifies Hematek rinse Indicates that there is power to the instrument Indicates low stain This symbol indicates that you should consult the instructions for use This is the fuse symbol This symbol indicates that the product is UL approved for safety United States and Canada This symbol indicates that the product is CSA approved for safety Canada This symbol indicates...

Page 91: ...dicates the name and location of the product manufacturer This symbol indicates the date of the manufacture of the product This symbol indicates the manufacturer s authorized representative within the European community This symbol indicates an in vitro diagnostic device This symbol informs you not to use the product if the package is damaged This symbol is intended to encourage recycling This sym...

Page 92: ...Page A 4 Appendix A List of Symbols This symbol indicates that the product or container should be oriented in the direction of the arrows ...

Page 93: ...ed if defective Is the slide drying system operating properly Are the conveyor spirals operating Is the LOW STAIN light operating properly Do all three stations pump reagent properly Do all stations pump reagent at the proper time Is the instrument level Has the platen been cleaned each day Has the stain tubing been cleaned each day Are the sensing fingers properly adjusted When was the pump tubin...

Page 94: ...Page B 2 Appendix B Hematek Preservice Checklist ...

Page 95: ... level 1 5 cleaning emptying the waste tank 5 6 platen 5 3 stain tubing and cannula 5 4 tubing after use 3 7 conveyor spirals loading slides 1 6 D daily cleaning platen 5 3 stain tubing and cannula 5 4 waste tank 5 6 decontamination 5 9 drain trough cleaning 5 6 drawer slide description 1 7 E environmental factors humidity range 2 3 temperature range 2 3 F feet levelers 1 5 fuse replacement proced...

Page 96: ...ime the tubing 3 4 stain the smears 3 6 start the instrument 3 3 turn the instrument off 3 7 operation theory of 1 4 opposing grooves 1 6 ordering supplies and replacement parts 8 10 P parallel spirals conveyor 1 6 peripheral blood smears 4 3 physical characteristics 1 5 platen cleaning procedure 5 3 description 1 6 main functions 1 6 position of blood smear 1 6 power light electrical system 1 12 ...

Page 97: ... 1 13 weight 1 13 specimens overview 4 3 spirals conveyor 1 6 Stain Pak replacement 3 7 stain tubing cleaning 5 4 staining the smears 3 6 stain to buffer ratio 6 18 starting the instrument 3 3 supplies Hematek cannula set 8 8 Hematek pump tube set 8 9 Hematek Stain Pak Modified Wright s Stain 8 8 Modified Wright Giemsa stain 8 8 Hematek underplaten tubing 8 9 ordering information 8 10 symbols used...

Page 98: ...Index 4 ...

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