Sartorius Stedim Biotech MD8 airscan Operating Instructions Manual Download Page 32 | Manualshive

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Sartorius Stedim Biotech MD8 airscan Operating Instructions Manual Download Page 32

9. How to use Gelatin Filter Units for air sampling

Gelatin filter units consist of 80 mm diameter gelatin filters which are fitted between the top
and base parts of a disposable plastic holder. They are individually packed in single or triple
bags and presterilized by gamma irradiation. Full details are given in the Directions for Use
supplied with them (publication number SM-6043-p).

9.1 How to fit a filter unit to the adapter

With a single-packaged filter unit, cut open the polyethylene bag on the side where the seam
is located (Fig. 10).

Fig. 10

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Summary of Contents for MD8 airscan

Page 1: ...85030 511 25 Operating Instructions Air Sampler MD8 airscan Ver 05 2011...

Page 2: ...Read these operating instructions carefully before switching the instrument on 2...

Page 3: ...ocedure 30 8 2 Resetting of default values 31 8 3 Service menu 31 3 9 How to use Gelatin Filter Units for air sampling 32 9 1 How to fit a filter unit to the adapter 32 9 2 How to remove a filter unit...

Page 4: ...e proven air samplers is manufactured for quality by high standard German electrical and precision engineering We are sure that it will provide you with trouble free service for many years Please do n...

Page 5: ...e and count the resulting number of colony forming units cfu This number is related to the volume sampled to give cfu m3 When the virus concentration is to be determined the gelatin filter must be dis...

Page 6: ...tarting the system check that the electrical supply is appropriate to that imprinted on the identification label attached to the base of the sampler 5 Ensure that the air outlet exhaust is completely...

Page 7: ...number 16746 conforms in its design and construction to the requirements of the following standards EN 50081 1 EN 55011 Cl B EN 50082 2 EN 61000 4 2 3 4 6 EN 50204 Status February 1997 This declarati...

Page 8: ...ent supplied for such damage while unpacking it Check the equipment supplied against the following list to ensure that everything has been correctly delivered and that nothing is left in the packaging...

Page 9: ...3 h 75 00 l min 5 0 m3 h 83 33 l min 5 5 m3 h 91 67 l min 6 0 m3 h 100 00 l min 6 5 m3 h 108 33 l min 7 0 m3 h 116 67 l min 7 5 m3 h 125 00 l min 8 0 m3 h 133 33 l min Correction of the air When the e...

Page 10: ...e Residual moisture Approx 46 49 Thickness Approx 250 m Air flow rate per cm2 2 2 3 2 l min at p 0 05 bar Temperature limit 60 C Limiting operating Max 30 C ambient temperature conditions max 85 relat...

Page 11: ...6 Description of the MD8 airscan The air inlet opening for attachment of a filter holder projects out of the plastic housing Fig 1 at the front of the MD8 airscan Fig 1 11...

Page 12: ...Fig 2 together with the combination of power plug socket and green on off switch The two fuses 3 15 AT for 230 V and 6 3 AT for 115 V are also situated in this switch An optional remote control interf...

Page 13: ...operating elements are on the top of the instrument Fig 3 with the LCD on the left of the carrying handle and the membrane covered keypad on the right The use of these is described in section 7 Fig 3...

Page 14: ...es including a length of flexible hose 17085 or 17662 and a set of connectors 17657 Other accessories such as adapters 17016 or 17030 clamps for sanitary flanges 17033 and filter capsules 5181307T9 SS...

Page 15: ...Connnecting the hose to the MD8 airscan Press the connecting piece 17658 hose MD8 airscan against the sampled air inlet of the MD8 airscan and turn it clockwise Fig 5 Fig 5 15...

Page 16: ...Fit one end of the hose e g 17085 onto the hose barb of the connecting piece 17658 and the other end onto the second connecting piece 17659 of the connecting set 17657 Fig 6 Fig 6 16...

Page 17: ...units 17801 Fig 7 or the filter holder 17655 containing a gelatin membrane filter onto the second connecting piece 17659 by turning it clockwise Fig 7 Use the clamp 17037 and clamp holder 16976 to fi...

Page 18: ...Fix a gelatin filter unit to the adapter Fig 8 In principle sampling can now be started 18...

Page 19: ...nd messages are shown on an LCD with 2 16 characters Fig 9 The keys of the keypad have the following functions Selects parameters to be displayed so that values can be checked and changed Enter Stores...

Page 20: ...the memory chips of the system are checked During this test the message sartorius MD8 selftesting is displayed flow chart 1 Upon successful completion of the selftest the message sartorius MD8 ready t...

Page 21: ...on Sartorius MD8 self test self test ok Sartorius MD8 Ready to start START KEYS Error Self test not ok Test running Change parameter Change Sample time Change Air flow rate Change Filter class Calibr...

Page 22: ...and checking or changing parameter settings by pressing the parameter key When is first pressed the message sampling time min is displayed Flow chart 2 Parameter Input 22 Parameter menu Sampling time...

Page 23: ...ly briefly pressed the value will change by 1 0 1 for air flow rate 0 01 for calibration values If you hold down one of these keys the value displayed will automatically be increased or decreased by 1...

Page 24: ...ests recalibration When a displayed parameter is changed with the or key the new setting flashes to show it has not been stored If you wish to store the change make sure to press Enter If you exit the...

Page 25: ...is displayed On pressing the Enter key the initial message sartorius MD8 ready to start is displayed Should it be necessary for you to interrupt sampling ahead of time press the Stop key This stops t...

Page 26: ...u simply use this key to recall each parameter the same way as when checking or changing the individual parameters one after the other The only difference is that you cannot change the parameter setti...

Page 27: ...values are displayed but you cannot change them Returns to display mode 10 sec after the key was fast pressed The time that sampling already has taken up until interruption is rounded to minutes and d...

Page 28: ...to the time the air sampling procedure is aborted is displayed Error message maximum airflow m3 h Enter 7 6 Changing the language English or German When the MD8 airscan is in the initial starting posi...

Page 29: ...ted Exact details on how to do this are given in section 5 of the operating instructions provided with the calibration unit publication number SL 6051a A complete reference curve for the flow rate sen...

Page 30: ...his on the MD8 airscan using the or key then confirm and store it with Enter 5 If this is not the last calibration point the next one is automatically called up and process 1 4 is repeated When the 7...

Page 31: ...ssing of the key must be made while the self testing message is displayed as only then will the display change to delete data Start yes By now pressing ENTER you confirm this and all data will be over...

Page 32: ...able plastic holder They are individually packed in single or triple bags and presterilized by gamma irradiation Full details are given in the Directions for Use supplied with them publication number...

Page 33: ...e the seam is located Remove the filter unit by hand touching only the outer edge of the unit Fig 11 and lock it onto the adapter which is either subsequently attached or was previously attached to th...

Page 34: ...Fig 12 The adapter order no 17801 can be locked onto the air sampler inlet or the hose connector by turning it clockwise either before or after the filter unit is attached Fig 12 34...

Page 35: ...detach the top part of the filter unit by turning it counterclockwise Fig 14 Invert an agar filled petri dish base over the gelatin filter Fig 15 and make contact so that the gelatin filter can adher...

Page 36: ...Fig 14 Fig 15 36...

Page 37: ...Fig 16 Fig 17 37...

Page 38: ...Fig 18 38...

Page 39: ...croorganisms After transferring the gelatin filter to the culture medium incubate it with the petri dish lid facing up to avoid condensed water on the lid The relative humidity of the air in the incub...

Page 40: ...Fig 19 40...

Page 41: ...er cubic meter of air by relating the colony count obtained to the volume of air sampled cfu m3 We recommend that you plate the gelatin filter on a suitable agar immediately after sampling is complete...

Page 42: ...prayed with disinfectants or where antibiotic dust could be present subsequent filtration of the solution through an 0 45 m cellulose nitrate membrane filter e g 11406 and rinsing this with an osmotic...

Page 43: ...onsists of a top and a bottom part Fig 20 The top part fits on the bottom part by turning it clockwise all the way to the stop Before first use clean the parts with hot water If necessary add a little...

Page 44: ...biological work sterilize the filter holder or assembled stack in a drying oven at 180 C for up to 2 hours Autoclaving is not possible since the aluminium material would corrode on account of the mois...

Page 45: ...be used on the filter support of the bottom part centering it properly Fig 21 Close the holder with the bayonet catch without damaging or displacing the filter Wrap the holder in aluminium foil or sim...

Page 46: ...Detach the filter holder containing the exposed filter from the air inlet of the sampler Fig 22 or the connection on the hose and protect it from contamination until the gelatin filter is removed for...

Page 47: ...sing the aluminium filter stack An aluminium filter stack accessory order no 17656 Fig 23 consists of 10 filter holders fitted together in a stack in which used and unused holders can be separated Fig...

Page 48: ...e stack place the holders in numerical order and insert the top of the first holder in the intermediate adapter that accepts holders on either side Fig 24 Fit the top of the second holder in the botto...

Page 49: ...herical retainers be somewhat stiff at any time they can be loosened by using a special alkaline detergent in an ultrasonic bath details on request For microbiological work sterilize in a drying oven...

Page 50: ...mediate adapter to the top part of the second filter holder Fit the filter holder on the MD8 airscan or the connecting piece 17659 see section 6 After sampling remove the holder used fit it on the oth...

Page 51: ...25 hose barb to 1 11 Sanitary flange to connect MD8 airscan to an isolator via silicone tubing and a filter capsule 17030 Adapter DN 30 hose barb to 1 11 Sanitary flange to connect MD8 airscan to an...

Page 52: ...17655 17656 17657 17657 17657 17208 52...

Page 53: ...17085 17088 17662 16970 16970 16970 53...

Page 54: ...17801 17016 17030 17033 5185307T9 SS A 54...

Page 55: ...e polyethlyene bags but label on innermost bag 12602 080 ALK Gelatin disc filters 80 mm diameter pack of 50 presterilized 17528 080 12602 080 ALK Under conditions where gelatin filters cannot be used...

Page 56: ...itions can differ somewhat in various countries Should no guarantee card be included with the instrument please contact the Sartorius Stedim Biotech subsiduary in your country at the address given on...

Page 57: ...781 Aubagne Cedex Phone 33 442 845600 Fax 33 442 845619 Sartorius Stedim Italy S p A Via dell Antella 76 A 50012 Antella Bagno a Ripoli FI Phone 39 055 63 40 41 Fax 39 055 63 40 526 Sartorius Stedim J...

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