Roche MagNA Pure Compact Instrument, Operator'S Manual

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MagNA Pure Compact Operator’s Manual - Version 1.3 94
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4.3 Trouble Shooting Guide
Problem Possible cause Recommendation
Drops of liquid on
the dropcatcher or
the stage. Leakage of reaction tips due to
damaged or worn nozzle O-rings Check O-rings and exchange, if necessary
Run stopped due to
head crash sensor
activation Disposables were not inserted
correctly or any other obstacle is
in the way of the nozzle head
䉴
Follow the instructions about placement of
accessories and disposables
䉴
Check reaction tips
䉴
Check even alignment of reaction tips when
attached to the nozzles.
Pipetting of wrong
liquid volumes
䉴
Reagent Rection Tips might
be bent.
䉴
Reaction tips not attached
correctly to the nozzles
䉴
Instrument misadjusted.
䉴
Check reaction tips
䉴
Check O-rings of nozzle head
䉴
Call Roche service for Easy Teaching
Clumping of beads
or beads in eluate Too much sample material was used Reduce amount of sample material to the values
recommended in the pack insert.
Magnetic glass particles (MGPs)
were magnetized prior to use Avoid contact of the MGPs with magnets prior to use.
Degraded nucleic
acid Inappropriate storage of samples Use fresh or frozen samples; avoid using samples that
were stored at room temperature for extended periods
of time.
Incorrect storage of eluate Do not store eluates at room temperature;
do not perform overnight runs.
Nuclease contamination due to
improper handling Keep instrument clean and use nuclease-free pipettes
and tips. Wear gloves.
Poor DNA yield Sample does not contain enough
cells Cultured cells: Count cells before use. Optimal results
are obtained with 10
5
-10
6
cells.
Blood: Do not use clotted or sedimented blood.Use
anticoagulated fresh or frozen blood. Mix tubes before
use. Count cells before use.
Storage of samples was
not optimal Use fresh or frozen samples; avoid using samples that
were stored at room temperature for extended periods
of time.
Poor DNA purity
(low ratio OD
260/280 nm) Too much sample material Reduce amount of sample material to the values
recommended in the pack insert.
Storage of samples was
not optimal Use fresh or frozen samples; avoid using samples that
were stored at room temperature for extended periods
of time.
Error Codes and Trouble Shooting Guide
Trouble Shooting Guide
MagNA Pure Compact Manual.book Seite 94 Dienstag, 28. Februar 2006 4:16 16