Retsch MM 500 Vario, Manual

Page: 40 / 87

Operating the Device
40
Dry grinding
Recommended number of
grinding balls
Wet grinding
Recommended number of
grinding balls
Grinding jar
size
Sample volume Max. feed size Ø
15 mm
Ø
20 mm
Ø
25 mm
Ø
≤ 3 mm
1.5 ml 0.2
–0.5 ml
1 mm
- -
-
Stainless steel
: 4.5 g
5 ml 0.5
–2 ml
2 mm
- -
-
Stainless steel
: 15 g
10 ml 2
–4 ml
4 mm
- -
-
Zirconium oxide
: 20 g
Stainless steel
: 30 g
Tungsten carbide
: 55 g
25 ml 4
–10 ml
6 mm
1 - 2 -
-
Zirconium oxide
: 55 g
Stainless steel
: 75 g
Tungsten carbide
: 140 g
35 ml 6-15 ml 6 mm
2 - 3 1
-
Zirconium oxide
: 75 g
Stainless steel
: 105 g
50 ml 8-20 ml 8 mm
3 - 4 1
1
Stainless steel
: 145 g
The MM 500 Vario allows the use of 1.5 ml / 2 ml / 5 ml reaction tubes.
Dry grinding
Recommended number of grinding
balls
Stainless steel or Zirconium oxide
Cell disruption of
biological cells
Grinding jar
size
Sample
volume
Max. feed
size
Ø
4 mm
Ø
5 mm
Ø
7 mm
Ø
10 mm
Glass beads (0.1-0.25
mm/0.25-0.5 mm/0.75-
1 mm/1-1.5 mm)
Zirconium oxide grinding
balls (< 3 mm)
1.5 ml 0.2
–0.5 ml
<1 mm 2-4 - - - ~ 0.75 ml
2 ml 0.3-0.75 ml <2 mm 3-6 2-4 1-2 - ~ 1 ml
5 ml 0.5
–2 ml
<2 mm 12 - - - ~ 2.5 ml
6.4 Special grinding methods
6.4.1 Cryogenic grinding
WARNING
W8.0000
Risk of injury caused by liquid nitrogen
Use of liquid nitrogen during cryogenic grinding
−
Liquid nitrogen has a boiling point of
– 196 °C and causes burn-like
injuries and frostbite if there is skin and eye contact.
•
Take note of the liquid nitrogen safety data sheets.
•
Always wear goggles and protective gloves when using liquid
nitrogen.