KrosFlo® TFDF™ Lab System
User Guide
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repligen.com IF.UG.023 R1
Figure 19. Experiment workflow
Running a KrosFlo® TFDF™ experiment is made simple by the Wizard feature. Enter just 5 inputs to
automatically generate run parameters:
•
%PCV
•
Max pool volume
•
Target yield
•
Starting volume
•
Initial concentration factor
Typically, the most important experimental parameter is the %PCV. While all samples have their
unique properties, it is generally found that samples with a %PCV below 25% may be run directly.
Samples with %PCV > 25 may require either early diafiltration or an initial dilution of the feed
material in buffer or media. Most samples meet recovery and turbidity reduction expectations with
Wizard feature derived parameters and without optimization. If turbidity breakthrough is observed,
it is recommended to increase volumetric expansion by 5 - 10% and reduce the initial concentration
factor. If filter fouling is observed, it is recommended to verify the %PCV measurement. If %PCV is
confirmed to be < 25%, reduction of the flux rate should be considered. If the %PCV is greater than
25%, trials with either early diafiltration or an initial dilution are potential steps towards
improvement. If early diafiltration is implemented, recommended starting parameters are running in
C/D mode with a concentration factor between 1 - 1.5.
