KrosFlo® TFDF™ Lab System
User Guide
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repligen.com IF.UG.023 R1
Figure 2. KrosFlo® TFDF™ Lab System Components
Controller
Users change parameters and monitor processes through the interface of the Controller. A 12-inch
touch screen enables user input, such as a change in pump speed and displays a diagram of the
system for visual analysis. The Wizard feature within the KrosFlo® TFDF™ Software, assists running
an optimum process. Based on just a few user specifications, the KrosFlo® Wizard feature generates
run parameters for simple and accurate operation.
Figure 3. Controller
1.
12” Touchscreen interface
2.
Control panel
Pump Station
The Pump Station transfers all liquids during the TFDF™ process. A low shear and digitally controlled
magnetic levitating centrifugal style pump delivers feed stock from the bioreactor vertically through
the lumen of the TFDF™ filter and then back into the bioreactor. The Permeate (top) peristaltic
pump transfers permeate from the filter enclosure to the permeate reservoir. The Diafiltration/Aux
peristaltic pump (bottom) transfers buffer from a diafiltration reservoir to the bioreactor during the
diafiltration stage. The overall process is monitored using multiple sensors:
•
Three in-line single-use pressure sensors for feed, permeate and retentate
•
A non-invasive, clamp-on ultrasonic retentate flow meter
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5
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2
1.
Controller
2.
Pump Station
3.
Scale (for bioreactor)
4.
Scale (for permeate)
5.
ProConnex® TFDF™ Flow Path
