KrosFlo® TFDF™ Lab System
User Guide
29
repligen.com IF.UG.023 R1
KrosFlo® TFDF™ process
The typical KrosFlo® TFDF™ experiment is a three-step process consisting of a draw down step, a
wash step and finishing with a second draw down step.
Step 1, Draw Down
: The cell culture feed
stock is pumped from the bioreactor
through the lumen of the TFDF™ filter.
Retentate from the filter (blue) circulates
back to the bioreactor while permeate
from the filter (red) is routed to the
permeate reservoir (red) with the
permeate peristaltic pump operating in
the clockwise direction. At the end of Step
1, approximately
50%
of the original cell
culture volume has been transferred to
the permeate reservoir and the cell
culture feed has been effectively
concentrated.
Step 2, Wash
: Diafiltration/Wash buffer
(purple) is pumped into the bioreactor
while circulation of the cell culture feed
stock continues. Permeate continues to
accumulate in the permeate reservoir. At
the end of Step 2, the permeate reservoir
volume reaches approximately
100%
of
starting cell culture volume. TMP is
expected to decrease slightly.
Set-up
: At set-up, the cell culture feed
starts in the bioreactor (blue). The
permeate reservoir is empty and the buffer
wash (purple) reservoir contains a volume
equal to approximately
50%
of the cell
culture feed volume.
