Qiagen QIAsymphony AS, Operating Manual

Page: 71 / 216

QIAsymphony SP/AS Consolidated Operating Guide 12/2017 71
The reagent cartridge contains sufficient reagents for up to 192 samples, depending on the kit
being used. Troughs of partially used reagent cartridges should be sealed immediately after use
with Reuse Seal Strips (provided in the QIAsymphony Kit).
Important
Do not refill partially used reagent cartridges as this may lead to performance
and pipetting errors.
Important
The length of time that the reagent cartridge is open must be kept as short as
possible.
All reagent troughs and enzyme racks are labeled at the side with the name of the buffer
contained in the trough. A unique 2D bar code on top of each trough enables the QIAsymphony
SP to detect the reagent cartridge and the contents of each trough.
The composition of the reagent cartridge is kit-specific. Do not mix troughs from different kits.
Visually check all reagent troughs for precipitates. If precipitates are present, refer to the
handbook of the QIAsymphony Kit you are using for more information.
Important
Make sure that reagents and enzymes are at room temperature (15–25°C)
before placing into the “Reagents and Consumables” drawer.
Important
Do not autoclave a prefilled reagent cartridge. Do not change the order of the
troughs within the reagent cartridge.
Important
Avoid shaking the reagent cartridge since this may cause buffers to foam,
resulting in liquid-level detection errors.
8.4.3 Buffer bottle
Depending on the kit being used, an additional bottle of buffer may be provided. The bottle is
prefilled with up to 60 ml of reagent.
To load the QIAsymphony SP with the buffer bottle, follow the steps below.
1. Remove the screw-cap from the buffer bottle.
2. Press Bottle ID in the Load Reagents screen.