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QIAamplifier 96 User Manual 03/2020

Select the program for quick start in the table and press

Start

to start the selected program. If

necessary, the program can be edited before the start or a new program can be created based on
a template (see chapter 8.1).

Start

Press the corresponding button to activate the programming mode or select a program from the list
of templates:

Edit

New from template

Summary of Contents for 1120412

Page 1: ...Sample to Insight__ March 2020 QIAamplifier 96 User Manual For Molecular Biology Application Not for use in diagnostic procedures QIAGEN GmbH QIAGEN Strasse 1 D 40724 Hilden 1120412...

Page 2: ...l 11 2 5 Safety instructions transport and installation 12 2 6 Safety instructions during operation 12 2 7 Behavior during emergencies 14 3 Technical Specifications 15 4 Function and setup 16 4 1 High...

Page 3: ...g editing and saving programs 46 8 1 Programming modes 46 8 2 Creating a new program using a program template 48 8 3 Assigning program names 50 8 4 Setting the heated lid temperature 50 8 5 Preheating...

Page 4: ...hooting 107 13 1 Administrator password forgotten 107 13 2 Slow heating and cooling 107 13 3 Auto restart 107 13 4 Auto restart without an apparent cause 107 13 5 Adaptation of programs from other the...

Page 5: ...QIAamplifier 96 User Manual 03 2020 5 Waste Electrical and Electronic Equipment WEEE 116 Index 118 Document Revision History 120...

Page 6: ...theoretical expertise in molecular biology and the use of QIAGEN products If you have any questions or experience any difficulties regarding QIAamplifier 96 or QIAGEN products in general please do no...

Page 7: ...n 1 3 Intended use of QIAamplifier 96 The QIAamplifier 96 is an end point thermal cycler It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PC...

Page 8: ...r several of the following causes Use of the QIAamplifier 96 other than intended Improper commissioning operation and servicing of the device Modifications of the QIAamplifier 96 without prior consult...

Page 9: ...References to potential dangers do not replace the work protection regulations which must be observed 2 1 Symbols and signal words used This manual uses the following symbols and signal words to indi...

Page 10: ...actions leading to personal injury or material damage Important Do not remove safety symbols Replace damaged safety symbols immediately The following safety symbols are attached to the inside of the...

Page 11: ...ion The technical condition must always comply with the legal requirements and regulations Prior to every use the device must be checked for damage and sound condition Any changes in the device affect...

Page 12: ...ach commissioning that the condition of the device including the safety equipment is sound Observe the following Free access to the power switch on the back of the enclosure has to be ensured during o...

Page 13: ...by the customer service of QIAGEN and specially authorized technicians The electrical components must be checked regularly by a qualified electrician Any defects such as loose connections and faulty...

Page 14: ...ial 2 6 5 Safety instructions service and repair The QIAamplifier 96 is usually repaired by the service department of QIAGEN or its authorized and trained specialist personnel Unauthorized repairs can...

Page 15: ...e info file SINF generation expanded self test adjustable ramp rates gradient temperature diagram view Program memory Total capacity of 350 programs in up to 90 user directories Language English Displ...

Page 16: ...ns on a PC Figure 1 The QIAamplifier 96 Thermal Cycler The QIAamplifier 96 thermal cycler features an automatic restart If a power failure occurs during the run the device will continue the run as soo...

Page 17: ...ve the block surface level and it ensures reliable contact between the reaction tubes and the thermoblock by applying constant pressure Thanks to the integrated clutch mechanism the pressure applied t...

Page 18: ...f time if for each single step all parameters have to be set manually The QIAamplifier 96 software offers several pre installed program templates for different applications The program templates provi...

Page 19: ...ubes strips or plates PCR plates can be standard profile or low profile and they can be skirted half skirted or non skirted The QIAamplifier 96 can handle all kinds of plates and the plasticware can b...

Page 20: ...ttempt to operate the device if there is any visible damage Keep the original packaging for possible return The QIAamplifier 96 must always be transported in its original packaging If the packaging is...

Page 21: ...QIAamplifier 96 User Manual 03 2020 21 Figure 1 Voltage selector switch on the underside of the device...

Page 22: ...tion Place the device at a minimum distance of 10 cm between the rear and sides of the device and the wall or other objects Place the QIAamplifier 96 on a solid dry surface If the QIAamplifier 96 has...

Page 23: ...r 96 User Manual 03 2020 23 6 Control elements 6 1 QIAamplifier 96 front view 1 Dial for adjusting the contact pressure 2 Lid sealing button 3 Display 4 USB port Figure 2 Front view of the QIAGEN QIAa...

Page 24: ...6 2 QIAamplifier 96 rear view The Ethernet port mains plug and mains switch are located at the rear of the QIAGEN QIAamplifier 96 1 Ethernet port 2 Mains switch 3 Fuse holder 4 Mains plug Figure 3 Re...

Page 25: ...e until the clutch mechanism is activated you will hear a clicking noise ATTENTION The strength of the lid contact pressure has been designed for a fully populated block If only a few samples are to b...

Page 26: ...elow when opening the lid If the lid is opened under pressure the locking mechanism may be damaged 1 Reduce the contact pressure of the lid completely by turning the lid wheel counterclockwise until t...

Page 27: ...ation 6 4 1 Keyboard For some applications names addresses or passwords have to be defined For this purpose the QIAamplifier 96 software offers a keyboard to enter characters digits and special charac...

Page 28: ...maximum of 3 characters 6 4 2 Keypad In some screens of the QIAamplifier 96 software digits have to be entered For this purpose the software features a keypad integrated in some screens Figure 6 QIAa...

Page 29: ...igher than the maximum number that can be displayed on a screen the software will show cursor keys for navigation Scroll up Scroll right Scroll down Scroll left 6 4 4 Confirm or cancel On many screens...

Page 30: ...30 6 4 5 List of commands and icons used The QIAamplifier 96 software uses the following icons Command Icon Home Back Right Left Up Down Confirm Cancel Login User management New user Delete user Edit...

Page 31: ...rom previous page Command Icon Incubation Start incubation Programs New program Open template Copy program copy all programs Delete program Edit program Start program Quick select program or user Stop...

Page 32: ...inued from previous page Command Icon Pause program Continue program Skip step Graphical view Spreadsheet view Gradient display Edit step Insert step Delete step Time increment Heating rate Gradient L...

Page 33: ...m previous page Command Icon Standard gradient programming Preheating OFF Preheating ON Tools Settings Date and time Automatic user logout Beeper Network Display brightness Display darker Display brig...

Page 34: ...n Factory settings Documentation Run log file Run log file overview Save run log file to USB Save all run log files to USB View run log file View program Log file self test Error log file Extended sel...

Page 35: ...QIAamplifier 96 User Manual 03 2020 35 Table continued from previous page Command Icon Backup file Save backup file Load backup file Cycler information...

Page 36: ...power on self test the login screen is displayed The login screen offers the following functions Command Function Chapter Login Starts the login dialog See chapter 7 3 Block Shows the current block s...

Page 37: ...the most recently logged in user is always shown in the first position at the top left of the screen All the other buttons become arranged from top left to bottom right according to the date and time...

Page 38: ...QIAamplifier 96 User Manual 03 2020 38 Figure 9 QIAamplifier 96 enter password screen...

Page 39: ...grams Viewing running and editing programs See chapter 8 Block Shows the current block status If a block is free a touch on the button leads to a list of previously used or changed programs for quick...

Page 40: ...haracters are shown in red and the warning Hot plus the corresponding warning symbol are displayed In the line below the textbox the remaining runtime is shown The sample block number is shown above t...

Page 41: ...QIAamplifier 96 User Manual 03 2020 41 Button Block status Color Free Green Running Yellow Pause Blue...

Page 42: ...e a touch of the button will lead to the program view screen instead If the block status button is used in the login screen before a user is logged in a list of the programs most recently used on the...

Page 43: ...tart to start the selected program If necessary the program can be edited before the start or a new program can be created based on a template see chapter 8 1 Start Press the corresponding button to a...

Page 44: ...ss Incubate and in the next screen set the values for the block temperature and hold time using the keypad Set whether the heated lid should be on or off and whether it should preheat or not using the...

Page 45: ...ds can be used Stop Pause Continue Skip Note To stop the incubation use the corresponding command Do not switch off the device while the incubation is running The device will react like after a power...

Page 46: ...Programming modes The QIAamplifier 96 software offers spreadsheet or graphical programming Use the buttons to toggle between the two modes The following screenshots show the spreadsheet and graphical...

Page 47: ...ode Chapter Program name See chapter 8 3 Heated lid temperature C See chapter 8 4 Preheat lid mode See chapter 8 5 Step number See chapter 8 6 Temperature C See chapter 8 6 Hold time h mm ss See chapt...

Page 48: ...1 2 After login press Program in the home screen see chapter 7 3 Program The program overview screen opens Figure 16 QIAamplifier 96 program overview screen 3 In the program overview screen press New...

Page 49: ...lifier 96 Blank program template Select Blank whenever a new program should be created and select another template from the list whenever an existing program should be edited The program structure can...

Page 50: ...6 4 1 Note If a character is pressed the pre displayed name will be deleted and a new program name can be set If the program name should be modified first press another button like delete or space 8...

Page 51: ...cessary cooling against the heating cover is required 8 5 Preheating the heated lid The heated lid can be set to preheating ON or OFF using the corresponding buttons Preheat heated lid Off Preheat hea...

Page 52: ...ch the target temperature is increased or decreased with each cycle The temperature increment can be 20 0 C See chapter 8 6 1 2 Time increment e g for long range PCR by which the time is increased wit...

Page 53: ...n Figure 20 QIAamplifier 96 program step editing screen Note To program a step at least the temperature and the hold time must be defined To set the temperature touch the corresponding input field and...

Page 54: ...g of loops For a loop two parameters have to be set 1 The step number to which the program should return Go To 2 The number of repetitions Cycles To program a loop select the last step of the loop and...

Page 55: ...n the graphical programming mode 8 6 1 3 Programming a time increment For some applications for example long range PCR time increments are used The time is increased in each cycle by the set value To...

Page 56: ...8 6 1 5 Programming a gradient step The gradient function is most often used to optimize new primer pairs A temperature gradient is established across the sample block in the annealing step so that t...

Page 57: ...sample block The effective temperatures for all the other columns are automatically calculated and displayed as a bar chart see Figure 23 Note The annealing temperature is set for a specific column i...

Page 58: ...sing the standard gradient programming screen enter a temperature for the first column and the last column in the sample block see Figure 24 The effective temperatures for all the other columns are au...

Page 59: ...l or spreadsheet view To edit a parameter for a step press directly on the cell for the program parameter in the table see Figure 25 Figure 24 QIAamplifier 96 spreadsheet program view Parameter Spread...

Page 60: ...mplifier 96 graphical program view Parameter Spreadsheet view Temperature gradient C input field Hold time h mm ss input field Loop counter option button Temperature increment option button Time incre...

Page 61: ...6 User Manual 03 2020 61 Figure 26 QIAamplifier 96 individual program parameter editing You also have the option of programming a gradient step To do this when editing the temperature press the Grad b...

Page 62: ...iting program steps opens see chapter 8 6 Enter the desired parameters for the new step Note The new step will be inserted at the selected program step If for example step 3 is selected the new step w...

Page 63: ...h the corresponding buttons to select a user directory and a program storage location If the number of user directories or programs is higher than the maximum number that can be displayed in the list...

Page 64: ...or a program location using the quick select function Touch the icon above the user or program view see Figure 29 Quick select function An overview screen opens in which a maximum of 30 users or 50 us...

Page 65: ...29 QIAamplifier 96 quick select screen Before saving the program there is still the possibility of changing the program name To do this press Save as and enter the new program name using the QIAamplif...

Page 66: ...a program to be started press Programs on the QIAamplifier 96 home screen see chapter 7 4 Programs The program overview screen opens with the directory of the logged in user preselected see Figure 31...

Page 67: ...ram before it is started To start the selected program press Start Start Figure 31 QIAamplifier 96 program overview screen with preview 9 2 Copying a program To copy a program touch the corresponding...

Page 68: ...ready in use the stored program will not be overwritten instead this program and all other following programs will be moved one position higher The storage location number for these programs will incr...

Page 69: ...Save Save Note The programs will be copied into the selected user directory beginning at the first free storage location Figure 33 QIAamplifier 96 program overview screen 9 4 Deleting a program To de...

Page 70: ...e by 1 9 5 Deleting all programs To delete all programs touch the corresponding buttons to select a user directory on the QIAamplifier 96 program overview screen see Figure 31 If the number of user di...

Page 71: ...s been started see chapter 9 1 the QIAamplifier 96 software can display the currently running program in a spreadsheet or graphical view In addition the curve of the gradient can be displayed in gradi...

Page 72: ...ifier 96 display during the run in a graphical spreadsheet and gradient view The following parameters are summarized in the headline in the table Program name Sample block temperature Estimated end He...

Page 73: ...the graphical view each step is divided into a heating phase and a hold time and both parts are highlighted separately Time and temperature increments and ramp rate adjustments cannot be shown in the...

Page 74: ...ime field Spreadsheet view Graphical view Figure 37 QIAamplifier 96 run display screen during a program pause Note Pressing the Pause button requires manual interaction The QIAamplifier 96 software al...

Page 75: ...am To continue a paused program press Continue on the QIAamplifier 96 run display screen see Figure 38 Continue The QIAamplifier 96 will continue the program from exactly the point at which it was pau...

Page 76: ...QIAamplifier 96 User Manual 03 2020 76 Figure 38 QIAamplifier 96 confirmation prompt After confirmation of the confirmation prompt the QIAamplifier 96 stops the current program...

Page 77: ...mentation Access to stored log files See chapter 11 3 Self Test Executes the extended self test See chapter 11 4 Service info file Creates service info files SINF See chapter 11 5 Backup Saves all fol...

Page 78: ...ork Defines network settings See chapter 11 1 4 Display brightness Adjusts the display brightness See chapter 11 1 5 Touch screen calibration Calibrates the touchscreen See chapter 11 1 6 Factory sett...

Page 79: ...ield and use the keypad on the right side of the date and time entry screen to enter digits The following buttons may also be helpful when completing the fields Delete single character Delete all char...

Page 80: ...button Automatic logout On Automatic logout Off If automatic user logout is switched on the time after which the user is logged out from the system can be set You can enter values between 30 and 60 mi...

Page 81: ...r configuration screen 11 1 4 Network The Network menu item can be used to configure basic settings or show a list of network users Press the corresponding button Network settings Network user 8 6 1 6...

Page 82: ...Delete all characters Go to the next input field Figure 44 QIAamplifier 96 network settings screen Note The network cable must be at least performance class Cat 5e and the cable configuration must be...

Page 83: ...slider Darker Brighter Figure 45 QIAamplifier 96 display brightness adjustment screen 11 1 6 Screen calibration To calibrate the screen follow the instructions and touch the screen at the center of th...

Page 84: ...prompt Figure 47 QIAamplifier 96 confirmation prompt Using the factory settings tool will delete all users and programs Use a backup file to restore the system see chapter 11 6 11 2 User management T...

Page 85: ...s Figure 48 QIAamplifier 96 user management screen Available users are listed on the QIAamplifier 96 user management screen see Figure 49 sorted by the date and time of the last login and the username...

Page 86: ...he QIAamplifier 96 keyboard see chapter 6 4 1 Username up to 13 characters or numbers Initials 2 to 3 characters Figure 49 QIAamplifier 96 create new user screen 11 2 2 Editing user settings To edit t...

Page 87: ...e first system start and to create a backup of the system Folders programs users and rights settings can be restored from a backup file 11 2 3 Delete user To delete a user select the user on the user...

Page 88: ...the following documentation tools Run Log File Documentation of PCR runs See chapter 11 3 1 Power On Log File Result of the latest initial self test See chapter 11 3 2 Self Test Log File Result of th...

Page 89: ...d is shown at the first position in the table The initials of the user who started the program the number of the sample block being used the program name the directory and the program number are also...

Page 90: ...rsion User initials Cycler type Serial number of the QIAamplifier 96 thermal cycler Sample block type Block serial number Block number Messages Figure 54 QIAamplifier 96 run log file screen To view th...

Page 91: ...en the run log file screen and the view program screen Overview Run Log Files View Run Log File Show Program To export all the saved run log files on USB press Save all Run Log Files on USB To export...

Page 92: ...and time Last power down by date and time A table with a list of error messages by number date time error code and message Figure 56 QIAamplifier 96 power on log file screen 11 3 3 Extended self test...

Page 93: ...n be Passed or Failed and is listed in the last row of the table for each test 11 3 4 Error log file The QIAamplifier 96 records all errors by date and time The latest error is shown at the first posi...

Page 94: ...at sink and the fans are working properly together Gradient Checks if the sample block reaches the set gradient temperatures Heated lid Tests if the heated lid reaches the set temperature and can hold...

Page 95: ...tended self test can be started To start the extended self test press Start Self Test Figure 60 QIAamplifier 96 extended self test screen The software provides a progress overview during the extended...

Page 96: ...mplifier 96 extended self test overview screen The results of the extended self test are summarized in a log file stored by the device and can be viewed by the user see chapter 11 3 3 11 4 Service inf...

Page 97: ...stored to USB and transferred to another QIAamplifier 96 Figure 62 QIAamplifier 96 backup file screen Connect a USB stick to the device and press Save backup file to create a backup file Save backup...

Page 98: ...ock type Block serial number Software version Protocol version Revision number power board Software version power board Software version power board logic Revision number processor board Software vers...

Page 99: ...QIAamplifier 96 User Manual 03 2020 99 Figure 65 QIAamplifier 96 Cycler Info Screen...

Page 100: ...tailed description of the error situation This information will help you and your QIAGEN Technical Service Specialist to deal most efficiently with your issue For technical assistance and more informa...

Page 101: ...and may come to an explosive boil Wear goggles when handling hot samples Make sure that the lid is closed securely before starting the program NOTICE The use of oil between samples and sample block is...

Page 102: ...Tools to access the system settings user management documentation backup tool or self test functions Press Logout to log out Editing saving and copying programs Press Programs on the QIAamplifier 96...

Page 103: ...id preheating on or off press the corresponding button Select a program step and press Edit The screen for editing program steps opens Use the cursor keys above the keyboard to switch between program...

Page 104: ...sheet or graphical program display Alternatively individual program parameters for a step can also be edited To do this in the spreadsheet view press directly on the cell for the parameter in the prog...

Page 105: ...n prompt Starting stopping and pausing programs The QIAamplifier 96 stores the latest 5 started or edited programs for each user To quick start a program press Quick Start on the QIAamplifier 96 home...

Page 106: ...aused program press Continue To skip a step press Skip The program will continue with the next step To stop an active program press Stop program and confirm the confirmation prompt Programs with a pau...

Page 107: ...o restart function If a power failure occurs during a run the device will continue the run at the same point when the power returns In case of long term power failure longer than 30 minutes the device...

Page 108: ...this situation the clutch mechanism is active in both directions there is a clicking noise in both directions To unlock the lid wheel press down the metal pin on the top of the lid wheel with a pen Th...

Page 109: ...vice department immediately In order to guarantee sound and safe operation and to ensure laboratory certification we recommend concluding a maintenance agreement with regular device validation 14 1 Cl...

Page 110: ...ing spray disinfectants there is a risk that the liquid enters the sensitive electronic system through the ventilation slits If the disinfectant has a spray nozzle apply disinfectant to a suitable clo...

Page 111: ...y damage caused by inadequate decontamination of the device Please clean all device components from biologically hazardous chemical or radioactive contamination see also section Disinfecting the devic...

Page 112: ...s an extra designed packaging system with inserts of foamed material Important The thermal cycler is only protected from transport damage if the original packaging is used and the packing instructions...

Page 113: ...dispose of any waste materials that occur during measurements sample materials in accordance with statutory and local regulations At the end of its service life the QIAamplifier 96 and all its electr...

Page 114: ...3 2020 114 Appendix A Declaration of Conformity Name and address of the legal manufacturer QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany An up to date declaration of conformity can be requested fr...

Page 115: ...QIAamplifier 96 User Manual 03 2020 115 Certificates For up to date certificates you may request them from QIAGEN Technical Services or from your local QIAGEN representative...

Page 116: ...ures that the product is recycled in a manner that protects human health and the environment Recycling can be provided by QIAGEN upon request at additional cost In the European Union in accordance wit...

Page 117: ...duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices interfacing...

Page 118: ...cubation 39 Liability 7 Login 33 Login screen 33 Maintenance 106 Manual Nomenclature 7 Operating voltage 20 Packaging 109 Password Administrator 34 Forgotten 104 Personnel 11 Power on self test 32 Pro...

Page 119: ...Display brightness 78 Factory settings 79 Network 76 Screen calibration 78 User management 79 Signal words 9 Step Skip 70 Symbols Manual 9 Tools 72 Contact 96 Cycler Info 95 Extended self test 90 Ser...

Page 120: ...QIAamplifier 96 User Manual 03 2020 120 Document Revision History Date Changes March 2020 Initial release...

Page 121: ...arties 2 Other than expressly stated licenses QIAGEN makes no warranty that this kit and or its use s do not infringe the rights of third parties 3 This kit and its components are licensed for one tim...

Page 122: ...QIAamplifier 96 User Manual 03 2020 122 1120412 HB 2739 001 03 2020 Ordering www qiagen com shop Technical Support support qiagen com Website www qiagen com...

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