QIAamplifier 96 User Manual 03/2020
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Sample replicates are distributed across the columns of the sample block and tested for the
temperature giving the optimal result. To program a gradient step press
Gradient
(see Figure 21). There are two options to program a gradient. To toggle between the two options,
press the corresponding button on the QIAamplifier 96 gradient programming screen (see Figure
23):
Linear Gradient Tool
Standard Gradient Programming
To program a gradient using the Linear Gradient Tool, enter the desired annealing temperature
(
Annealing Temp.
) and define a temperature increment (
Grad incr.
) between the individual columns
of the sample block. The effective temperatures for all the other columns are automatically calculated
and displayed as a bar chart (see Figure 23).
Note
: The annealing temperature is set for a specific column in the sample block. For example, the
annealing temperature in a 96 well sample block corresponds to the temperature in column 6. The
column number is indicated in parenthesis next to the corresponding input field (see Figure 23).
When setting a positive increment, the temperature in column 1 (left side of the sample block) is the
lowest and the temperature in column 12 (right side of the sample block) is the highest. Use the
minus button to enter a negative increment. When a negative value is used, the temperature in
column 1 (left side of the sample block) is the highest and the temperature in column 12 (right side
of the sample block) is the lowest.
